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Rabbit Recombinant Monoclonal LATS2 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Synthetic peptide - Human samples.

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Images

Immunoprecipitation - Anti-LATS2 antibody [EPR23126-488] - BSA and Azide free (AB280363), expandable thumbnail
  • Western blot - Anti-LATS2 antibody [EPR23126-488] - BSA and Azide free (AB280363), expandable thumbnail
  • Western blot - Anti-LATS2 antibody [EPR23126-488] - BSA and Azide free (AB280363), expandable thumbnail
  • Western blot - Anti-LATS2 antibody [EPR23126-488] - BSA and Azide free (AB280363), expandable thumbnail
  • Western blot - Anti-LATS2 antibody [EPR23126-488] - BSA and Azide free (AB280363), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-P
Human
Not recommended
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Synthetic peptide - Human
Not recommended
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species

Human, Mouse, Synthetic peptide - Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Synthetic peptide - Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide - Human, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Synthetic peptide - Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Version

    Target data

    Function

    Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor. Phosphorylates SNAI1 in the nucleus leading to its nuclear retention and stabilization, which enhances its epithelial-mesenchymal transition and tumor cell invasion/migration activities. This tumor-promoting activity is independent of its effects upon YAP1 or WWTR1/TAZ.

    Alternative names

    Serine/threonine-protein kinase LATS2, Kinase phosphorylated during mitosis protein, Large tumor suppressor homolog 2, Serine/threonine-protein kinase kpm, Warts-like kinase, KPM, LATS2

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    Rabbit Recombinant Monoclonal LATS2 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Synthetic peptide - Human samples.

    Alternative names

    Serine/threonine-protein kinase LATS2, Kinase phosphorylated during mitosis protein, Large tumor suppressor homolog 2, Serine/threonine-protein kinase kpm, Warts-like kinase, KPM, LATS2

    Key facts

    Isotype

    IgG

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogen
    • The exact immunogen used to generate this antibody is proprietary information.
    Carrier free

    Yes

    Clone number

    EPR23126-488

    Purification technique

    Affinity purification Protein A

    Concentration
    Loading...

    Storage

    Shipped at conditions

    Blue Ice

    Appropriate long-term storage conditions

    +4°C

    Notes

    ab280363 is the carrier-free version of Anti-LATS2 antibody [EPR23126-488] ab243657.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    Supplementary info

    Activity summary

    LATS2 also known as Large Tumor Suppressor kinase 2 is a serine/threonine-protein kinase with a molecular mass of approximately 121 kDa. It functions as a component of the Hippo signaling pathway where it helps regulate cell proliferation apoptosis and organ size. LATS2 shows expression in various tissues with notable levels in the lung heart and liver. Its role in these tissues makes it an important target for research in cancer and developmental biology.

    Biological function summary

    LATS2 plays an important role in cell cycle regulation and tumor suppression. LATS2 forms part of the core kinase complex within the Hippo pathway working closely with other proteins like MST1/2 kinases and Scaffold proteins SAV1. Through phosphorylation LATS2 can inhibit the activity of YAP and TAZ transcription co-activators promoting cell contact inhibition and proper tissue architecture. Its activity is important for limiting excessive cell growth and ensuring accurate cell division.

    Pathways

    LATS2 integrates into the Hippo signaling pathway and influences the PI3K-AKT signaling pathway. Within the Hippo pathway LATS2 interacts with MST1/2 forming an upstream kinase complex activating through phosphorylation of downstream transcription co-activators YAP and TAZ. These interactions result in the transcriptional regulation of genes controlling cell growth and apoptosis. Overlap with the PI3K-AKT pathway links LATS2 to cellular processes related to growth migration and survival providing a critical nexus that communicates cell status and environmental cues.

    Associated diseases and disorders

    LATS2 has associations with both cancer and cardiac hypertrophy. In cancer dysregulation of LATS2 activity can result in aberrant cell growth and tumor development often noted in different types of carcinomas where it can act as a tumor suppressor. Mutations or reduced expression of LATS2 can correlate with increased cancer progression implying a need for comprehensive understanding of its regulation and function. Additionally in cardiac hypertrophy LATS2 contributes to cardiac cell size regulation and its imbalance can connect to abnormal heart muscle thickening. In these contexts LATS2 interaction with proteins such as YAP and TAZ provides an important point of interest for therapeutic interventions.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

    5 product images

    • Immunoprecipitation - Anti-LATS2 antibody [EPR23126-488] - BSA and Azide free (ab280363), expandable thumbnail

      Immunoprecipitation - Anti-LATS2 antibody [EPR23126-488] - BSA and Azide free (ab280363)

      This data was developed using Anti-LATS2 antibody [EPR23126-488] ab243657, the same antibody clone in a different buffer formulation.

      LATS2 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate with Anti-LATS2 antibody [EPR23126-488] ab243657 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-LATS2 antibody [EPR23126-488] ab243657 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

      Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 ug

      Lane 2: Anti-LATS2 antibody [EPR23126-488] ab243657 IP in HeLa whole cell lysate

      Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-LATS2 antibody [EPR23126-488] ab243657 in HeLa whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 64 seconds

      Fresh lysates were used in this WB.

      All lanes: Immunoprecipitation - Anti-LATS2 antibody [EPR23126-488] (Anti-LATS2 antibody [EPR23126-488] ab243657)

      Predicted band size: 120 kDa

      Observed band size: 140 kDa

    • Western blot - Anti-LATS2 antibody [EPR23126-488] - BSA and Azide free (ab280363), expandable thumbnail

      Western blot - Anti-LATS2 antibody [EPR23126-488] - BSA and Azide free (ab280363)

      This data was developed using Anti-LATS2 antibody [EPR23126-488] ab243657, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      Fresh lysates were used in this WB.

      Exposure time: 3 minutes

      All lanes: Western blot - Anti-LATS2 antibody [EPR23126-488] (Anti-LATS2 antibody [EPR23126-488] ab243657) at 1/1000 dilution

      Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

      Lane 2: HEK-293T (human embryonic kidney epithelial cell), whole cell lysate at 10 µg

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

      Predicted band size: 120 kDa

      Observed band size: 140 kDa

    • Western blot - Anti-LATS2 antibody [EPR23126-488] - BSA and Azide free (ab280363), expandable thumbnail

      Western blot - Anti-LATS2 antibody [EPR23126-488] - BSA and Azide free (ab280363)

      This data was developed using Anti-LATS2 antibody [EPR23126-488] ab243657, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      Fresh lysates were used in this WB.

      This blot was developed using a higher sensitivity ECL substrate.

      Exposure time: 3 minutes

      All lanes: Western blot - Anti-LATS2 antibody [EPR23126-488] (Anti-LATS2 antibody [EPR23126-488] ab243657) at 1/1000 dilution

      Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), transfected with scrambled siRNA control, whole cell lysate at 20 µg

      Lane 2: HeLa transfected with siRNA specifically targeting LATS2, whole cell lysate at 20 µg

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

      Predicted band size: 120 kDa

      Observed band size: 140 kDa

    • Western blot - Anti-LATS2 antibody [EPR23126-488] - BSA and Azide free (ab280363), expandable thumbnail

      Western blot - Anti-LATS2 antibody [EPR23126-488] - BSA and Azide free (ab280363)

      This data was developed using Anti-LATS2 antibody [EPR23126-488] ab243657, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      Exposure time: 3 minutes

      All lanes: Western blot - Anti-LATS2 antibody [EPR23126-488] (Anti-LATS2 antibody [EPR23126-488] ab243657) at 1/1000 dilution

      Lane 1: His-tagged human LATS1 recombinant protein (aa701-1054) at 0.01 µg

      Lane 2: His-tagged human LATS2 recombinant protein (aa664-1016) at 0.01 µg

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

      Predicted band size: 120 kDa

    • Western blot - Anti-LATS2 antibody [EPR23126-488] - BSA and Azide free (ab280363), expandable thumbnail

      Western blot - Anti-LATS2 antibody [EPR23126-488] - BSA and Azide free (ab280363)

      This data was developed using Anti-LATS2 antibody [EPR23126-488] ab243657, the same antibody clone in a different buffer formulation.

      Western blot: Anti-LATS2 antibody [EPR23126-488] (Anti-LATS2 antibody [EPR23126-488] ab243657) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-LATS2 antibody [EPR23126-488] ab243657 was shown to bind specifically to LATS2. A band was observed at 117-171 kDa in wild-type A549 cell lysates with no signal observed at this size in LATS2 knockout cell line. To generate this image, wild-type and LATS2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

      All lanes: Western blot - Anti-LATS2 antibody [EPR23126-488] (Anti-LATS2 antibody [EPR23126-488] ab243657) at 1/1000 dilution

      Lane 1: Wild-type A549 cell lysate at 20 µg

      Lane 2: LATS2 knockout A549 cell lysate at 20 µg

      Lane 3: MCF7 cell lysate at 20 µg

      Lane 4: Raji cell lysate at 20 µg

      Secondary

      Lanes 1 - 4: Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

      Lanes 1 - 4: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

      Performed under reducing conditions.

      Observed band size: 117-171 kDa

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