AB192610

Anti-LAYN antibody [EPR11875(2)]

Name: Anti-LAYN antibody [EPR11875(2)] (ab192610) | Abcam
Brand: Abcam Limited
SKU: AB192610
Price: 265 USD
Availability: InStock
Rating: 4 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(3 Publications)

Rabbit Recombinant Monoclonal LAYN antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

UNQ208/PRO234, LAYN, Layilin

2 Images

Supplier Data

Western blot - Anti-LAYN antibody [EPR11875(2)] (AB192610)

All lanes:

Western blot - Anti-LAYN antibody [EPR11875(2)] (ab192610) at 1/10000 dilution

Lane 1:

Human fetal kidney lysate at 20 µg

Lane 2:

Human fetal liver lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 43 kDa

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Supplier Data

Western blot - Anti-LAYN antibody [EPR11875(2)] (AB192610)

All lanes:

Western blot - Anti-LAYN antibody [EPR11875(2)] (ab192610) at 1/1000 dilution

All lanes:

Human fetal brain lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 43 kDa

false

Carrier free

Anti-LAYN antibody [EPR11875(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11875(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The LAYN protein also known as layilin plays a role in cell adhesion and signal transduction. It is an approximately 55 kDa protein expressed mainly in epithelial and immune cells including T-cells and natural killer cells. This protein sits on the cell surface and has a connection to the cytoskeleton through its cytoplasmic tail. Alternate expression may occur in both normal and cancerous tissues indicating a role in various physiological and pathological processes.

Biological function summary

LAYN facilitates interactions between cells and the extracellular matrix. It acts as a receptor for hyaluronic acid contributing to cell migration and tissue remodeling. While it is not part of any specific complex layilin may interact with other extracellular matrix proteins to promote cellular activities. This interaction influences immune responses by modulating immune cell activation and homing.

Pathways

LAYN is involved in the hyaluronan-mediated motility pathway where it binds hyaluronic acid to regulate cell movement. Additionally the protein takes part in the immune response pathway by influencing immune cell behavior. LAYN interacts with proteins like moesin which helps connect the cell membrane to the actin cytoskeleton driving cellular changes during these processes.

LAYN has a connection to cancer progression and autoimmune diseases. It plays a role in the tumor microenvironment affecting tumor cell motility and can influence metastasis. In autoimmune disorders LAYN's role in modulating immune cell activity can trigger aberrant immune responses. The protein interacts with various components of the immune system linking its expression to both health and disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for hyaluronate.

See full target information LAYN

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e36595 PubMed39286102

2024

CREB1 promotes cholangiocarcinoma metastasis through transcriptional regulation of the LAYN-mediated TLN1/β1 integrin axis: CREB1 promotes cholangiocarcinoma metastasis through regulating LAYN/TLN1/β1 integrin axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Liang,Shuhua Zhou,Yangshuo Tang,Moyan Xiao,Ke Ye

Frontiers in cell and developmental biology 10:840171 PubMed35676936

2022

Integrated Analysis Reveals the Gut Microbial Metabolite TMAO Promotes Inflammatory Hepatocellular Carcinoma by Upregulating POSTN.

Applications

Unspecified application

Species

Unspecified reactive species

Yonglin Wu,Xingyu Rong,Miaomiao Pan,Tongyao Wang,Hao Yang,Xiejiu Chen,Zhenming Xiao,Chao Zhao

Neoplasma 68:262-272 PubMed33147052

2020

Upregulation of microRNA-650 by PBX1 is correlated with the development of Helicobacter pylori-associated gastric carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Junzheng Liu,Lei Wang,Jie Li,Yu Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LAYN antibody [EPR11875(2)]

Western blot - Anti-LAYN antibody [EPR11875(2)] (AB192610)

Western blot - Anti-LAYN antibody [EPR11875(2)] (AB192610)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise