AB251144

Anti-LAYN antibody [EPR11875(2)] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal LAYN antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

UNQ208/PRO234, LAYN, Layilin

2 Images

Supplier Data

Western blot - Anti-LAYN antibody [EPR11875(2)] - BSA and Azide free (AB251144)

This data was developed using ab192610, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LAYN antibody [EPR11875(2)] (<a href='/en-us/products/primary-antibodies/layn-antibody-epr118752-ab192610'>ab192610</a>) at 1/1000 dilution

All lanes:

Human fetal brain lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 43 kDa

false

Supplier Data

Western blot - Anti-LAYN antibody [EPR11875(2)] - BSA and Azide free (AB251144)

This data was developed using ab192610, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LAYN antibody [EPR11875(2)] (<a href='/en-us/products/primary-antibodies/layn-antibody-epr118752-ab192610'>ab192610</a>) at 1/10000 dilution

Lane 1:

Human fetal kidney lysate at 20 µg

Lane 2:

Human fetal liver lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 43 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11875(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab251144 is the carrier-free version of ab192610.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The LAYN protein also known as layilin plays a role in cell adhesion and signal transduction. It is an approximately 55 kDa protein expressed mainly in epithelial and immune cells including T-cells and natural killer cells. This protein sits on the cell surface and has a connection to the cytoskeleton through its cytoplasmic tail. Alternate expression may occur in both normal and cancerous tissues indicating a role in various physiological and pathological processes.

Biological function summary

LAYN facilitates interactions between cells and the extracellular matrix. It acts as a receptor for hyaluronic acid contributing to cell migration and tissue remodeling. While it is not part of any specific complex layilin may interact with other extracellular matrix proteins to promote cellular activities. This interaction influences immune responses by modulating immune cell activation and homing.

Pathways

LAYN is involved in the hyaluronan-mediated motility pathway where it binds hyaluronic acid to regulate cell movement. Additionally the protein takes part in the immune response pathway by influencing immune cell behavior. LAYN interacts with proteins like moesin which helps connect the cell membrane to the actin cytoskeleton driving cellular changes during these processes.

LAYN has a connection to cancer progression and autoimmune diseases. It plays a role in the tumor microenvironment affecting tumor cell motility and can influence metastasis. In autoimmune disorders LAYN's role in modulating immune cell activity can trigger aberrant immune responses. The protein interacts with various components of the immune system linking its expression to both health and disease states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for hyaluronate.

See full target information LAYN

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 22: PubMed34769092

2021

11,12 Epoxyeicosatrienoic Acid Rescues Deteriorated Wound Healing in Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Sommer,Heike Jakob,Caroline Reiche,Dirk Henrich,Jasmina Sterz,Johannes Frank,Ingo Marzi,Anna Lena Sander

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LAYN antibody [EPR11875(2)] - BSA and Azide free

Western blot - Anti-LAYN antibody [EPR11875(2)] - BSA and Azide free (AB251144)

Western blot - Anti-LAYN antibody [EPR11875(2)] - BSA and Azide free (AB251144)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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