Rabbit Polyclonal LBH antibody. Suitable for IHC-P, ICC and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LBH aa 1 to C-terminus.
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- EBioMedicine 48:36-482019Overexpression of Limb-Bud and Heart (LBH) promotes angiogenesis in human glioma via VEGFA-mediated ERK signalling under hypoxia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Jiang et. al.PubMed 31631037
- PeerJ 7:e68852019Elevated limb-bud and heart development (LBH) expression indicates poor prognosis and promotes gastric cancer cell proliferation and invasion via upregulating Integrin/FAK/Akt pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuoxi Yu et. al.PubMed 31119084
- Cellular physiology and biochemistry : internation 47:223-2342018Limb-Bud and Heart Attenuates Growth and Invasion of Human Lung Adenocarcinoma Cells and Predicts Survival Outcome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingming Deng et. al.PubMed 29788015
- OncoTargets and therapy 11:441-4482018Overexpression of LBH is associated with poor prognosis in human hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiewei Chen et. al.PubMed 29403288
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human LBH aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q53QV2
Reactivity data
Select an application| IHC-P | ICC | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
Target data
Function
Transcriptional activator which may act in mitogen-activated protein kinase signaling pathway.
Alternative names
Protein LBH, hLBH, Limb bud and heart development protein homolog, LBH
Recommended products
Rabbit Polyclonal LBH antibody. Suitable for IHC-P, ICC and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LBH aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human LBH aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q53QV2
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LBH also known as limb-bud and heart protein is a small protein with an approximate mass of 47 kDa. It plays a critical role in cell signaling and development processes by functioning as a transcriptional regulator impacting gene expression. Researchers discovered LBH's expression in several tissues including limb buds the heart and various epithelial tissues. This suggests its involvement in early developmental stages and tissue differentiation.
- Biological function summary
LBH influences the development of tissues by modulating gene expression during embryonic growth. It operates as part of a transcriptional complex that interacts with other transcription factors and cofactors. The influence of LBH on cellular differentiation aligns with its expression in developing organs suggesting its role in establishing cellular identities and organogenesis.
- Pathways
LBH integrates into signaling pathways related to developmental regulation such as the Wnt signaling pathway and TGF-beta pathway. LBH functions in conjunction with proteins like beta-catenin within the Wnt pathway which is important for cellular proliferation and differentiation. These pathways ensure a coordinated pattern of development linking LBH's activity to broader signaling networks that govern embryogenesis and tissue formation.
- Associated diseases and disorders
Alterations in LBH expression have connections with congenital heart defects and certain cancers illustrating its role in human health. Changes in LBH function or levels may disrupt normal development leading to conditions like atrial septal defects connected to aberrant interactions with transcription factors like T-box proteins. Furthermore its involvement in cancers suggests a link with proteins involved in cell cycle regulation hinting at potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Immunocytochemistry - Anti-LBH antibody (ab122223)
Immunocytochemistry analysis of RH-30 (human bone marrow metastasis cell line) cells labelling LBH with ab122223 at 2 μg/mL.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LBH antibody (ab122223)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling LBH with ab122223 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LBH antibody (ab122223)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling LBH with ab122223 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LBH antibody (ab122223)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastrointestinal tissue labelling LBH with ab122223 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LBH antibody (ab122223)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fallopian tube tissue labelling LBH with ab122223 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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