AB122223

Anti-LBH antibody

Name: Anti-LBH antibody (ab122223) | Abcam
Brand: Abcam Limited
SKU: AB122223
Price: 535 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal LBH antibody. Suitable for IHC-P, ICC and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LBH aa 1 to C-terminus.

View Alternative Names

Protein LBH, hLBH, Limb bud and heart development protein homolog, LBH

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LBH antibody (AB122223)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LBH antibody (AB122223)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastrointestinal tissue labelling LBH with ab122223 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LBH antibody (AB122223)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fallopian tube tissue labelling LBH with ab122223 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LBH antibody (AB122223)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling LBH with ab122223 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LBH antibody (AB122223)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling LBH with ab122223 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Immunocytochemistry - Anti-LBH antibody (AB122223)

Immunocytochemistry analysis of RH-30 (human bone marrow metastasis cell line) cells labelling LBH with ab122223 at 2 μg/mL.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC

applications

Immunogen

Recombinant Fragment Protein within Human LBH aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q53QV2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "0.25-2 µg/mL", "ICC-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LBH also known as limb-bud and heart protein is a small protein with an approximate mass of 47 kDa. It plays a critical role in cell signaling and development processes by functioning as a transcriptional regulator impacting gene expression. Researchers discovered LBH's expression in several tissues including limb buds the heart and various epithelial tissues. This suggests its involvement in early developmental stages and tissue differentiation.

Biological function summary

LBH influences the development of tissues by modulating gene expression during embryonic growth. It operates as part of a transcriptional complex that interacts with other transcription factors and cofactors. The influence of LBH on cellular differentiation aligns with its expression in developing organs suggesting its role in establishing cellular identities and organogenesis.

Pathways

LBH integrates into signaling pathways related to developmental regulation such as the Wnt signaling pathway and TGF-beta pathway. LBH functions in conjunction with proteins like beta-catenin within the Wnt pathway which is important for cellular proliferation and differentiation. These pathways ensure a coordinated pattern of development linking LBH's activity to broader signaling networks that govern embryogenesis and tissue formation.

Alterations in LBH expression have connections with congenital heart defects and certain cancers illustrating its role in human health. Changes in LBH function or levels may disrupt normal development leading to conditions like atrial septal defects connected to aberrant interactions with transcription factors like T-box proteins. Furthermore its involvement in cancers suggests a link with proteins involved in cell cycle regulation hinting at potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional activator which may act in mitogen-activated protein kinase signaling pathway.

See full target information LBH

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:109510 PubMed38660406

2024

The activation of LBH-CRYAB signaling promotes cardiac protection against I/R injury by inhibiting apoptosis and ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Anbiao Wu,Chongbin Zhong,Xudong Song,Wen Yuan,Mintian Tang,Tao Shu,Houda Huang,Pingzhen Yang,Qicai Liu

EBioMedicine 48:36-48 PubMed31631037

2019

Overexpression of Limb-Bud and Heart (LBH) promotes angiogenesis in human glioma via VEGFA-mediated ERK signalling under hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Jiang,Jinpeng Zhou,Dan Zou,Dianqi Hou,Haiying Zhang,Junshuang Zhao,Long Li,Jiangfeng Hu,Ye Zhang,Zhitao Jing

PeerJ 7:e6885 PubMed31119084

2019

Elevated limb-bud and heart development (LBH) expression indicates poor prognosis and promotes gastric cancer cell proliferation and invasion via upregulating Integrin/FAK/Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ruoxi Yu,Zhi Li,Chuang Zhang,Huicong Song,Mingming Deng,Liping Sun,Ling Xu,Xiaofang Che,Xuejun Hu,Xiujuan Qu,Yunpeng Liu,Ye Zhang

Cellular physiology and biochemistry : internation 47:223-234 PubMed29788015

2018

Limb-Bud and Heart Attenuates Growth and Invasion of Human Lung Adenocarcinoma Cells and Predicts Survival Outcome.

Applications

Unspecified application

Species

Unspecified reactive species

Mingming Deng,Ruoxi Yu,Shuo Wang,Ye Zhang,Zhi Li,Huicong Song,Bofang Liu,Ling Xu,Ximing Wang,Zhe Zhang,Qingjie Lv,Xiaonan Wang,Xiaofang Che,Xiujuan Qu,Yunpeng Liu,Xuejun Hu

OncoTargets and therapy 11:441-448 PubMed29403288

2018

Overexpression of LBH is associated with poor prognosis in human hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiewei Chen,Chuqiang Huang,Keming Chen,Shuman Li,Xinke Zhang,Jun Cheng,Muyan Cai,Yongbo Xiao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-LBH antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LBH antibody (AB122223)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LBH antibody (AB122223)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LBH antibody (AB122223)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LBH antibody (AB122223)

Immunocytochemistry - Anti-LBH antibody (AB122223)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise