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AB231182

Anti-LBP antibody

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(1 Publication)

Rabbit Polyclonal LBP antibody. Suitable for WB and reacts with Human, Recombinant full length protein - Cow samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Cow LBP aa 200 to C-terminus.

View Alternative Names

Lipopolysaccharide-binding protein, LBP

4 Images
Western blot - Anti-LBP antibody (AB231182)
  • WB

Supplier Data

Western blot - Anti-LBP antibody (AB231182)

All lanes:

Western blot - Anti-LBP antibody (ab231182) at 2 µg/mL

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Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate

Predicted band size: 53 kDa

false

Western blot - Anti-LBP antibody (AB231182)
  • WB

Supplier Data

Western blot - Anti-LBP antibody (AB231182)

All lanes:

Western blot - Anti-LBP antibody (ab231182) at 2 µg/mL

All lanes:

A549 (human lung carcinoma cell line) cell lysate

Predicted band size: 53 kDa

false

Western blot - Anti-LBP antibody (AB231182)
  • WB

Supplier Data

Western blot - Anti-LBP antibody (AB231182)

All lanes:

Western blot - Anti-LBP antibody (ab231182) at 2 µg/mL

All lanes:

Recombinant cow LBP

Predicted band size: 37 kDa,50 kDa,53 kDa,55 kDa,57 kDa,60 kDa,80 kDa

false

Western blot - Anti-LBP antibody (AB231182)
  • WB

Supplier Data

Western blot - Anti-LBP antibody (AB231182)

All lanes:

Western blot - Anti-LBP antibody (ab231182) at 2 µg/mL

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Predicted band size: 53 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Cow, Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Cow LBP aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q2TBI0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Cow": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lipopolysaccharide-binding protein (LBP) also known as LPS-binding protein is an acute-phase protein that plays a critical role in the immune response to Gram-negative bacterial infections. This protein weighing approximately 60kDa is primarily expressed in the liver and found in the serum. LBP binds to lipopolysaccharides (LPS) which are components of the outer membrane of Gram-negative bacteria facilitating the transfer of LPS to CD14 receptors on immune cells. This transfer enhances the recognition of bacterial endotoxins triggering an immune response.
Biological function summary

LBP serves a significant role in enhancing the sensitivity of the immune system to bacterial endotoxins. It does not form a permanent part of a larger complex but works closely with other molecules like CD14 and MD-2 an accessory protein of the TLR4 receptor to mediate the innate immune response. By efficiently binding LPS LBP aids in transporting these molecules to cell surface receptors accelerating the detection and subsequent response to infections.

Pathways

LBP is heavily involved in the TLR4 signaling pathway which is important for the detection and response to Gram-negative bacterial infections. Through this pathway LBP and associated proteins such as CD14 and MD-2 help activate TLR4 triggering downstream NF-κB signaling. This activation leads to the production of pro-inflammatory cytokines and an upsurge in antimicrobial activities fortifying the body's defenses against bacterial invasion.

Enhanced LBP levels have been associated with sepsis a severe systemic response to bacterial infections which can often lead to septic shock. Given its pivotal role in recognizing bacterial endotoxins LBP is important in identifying infections making it a potential prospect for therapeutic targeting. Additionally its interaction with CD14 means that alterations in LBP function or expression levels might contribute to immune dysregulation seen in inflammatory diseases such as Crohn's disease where immune responses to gut microbiota play a central role.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria (PubMed : 24120359, PubMed : 7517398). Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide (PubMed : 24120359, PubMed : 7517398).
See full target information LBP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 11: PubMed35119360

2022

α-/γ-Taxilin are required for centriolar subdistal appendage assembly and microtubule organization.

Applications

Unspecified application

Species

Unspecified reactive species

Dandan Ma,Fulin Wang,Rongyi Wang,Yingchun Hu,Zhiquan Chen,Ning Huang,Yonglu Tian,Yuqing Xia,Junlin Teng,Jianguo Chen
View all publications
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