Anti-LBP antibody [EPR10865]

• IP

Unknown

Immunoprecipitation - Anti-LBP antibody [EPR10865] (AB169776)

All lanes:

Immunoprecipitation - Anti-LBP antibody [EPR10865] (ab169776) at 1/1000 dilution

Lane 1:

Immunoprecipitation of LBP from Human plasma lysate using ab169776 at 1/10 dilution

Lane 2:

1 X PBS Negative control

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 53 kDa

false

• WB

Unknown

Western blot - Anti-LBP antibody [EPR10865] (AB169776)

All lanes:

Western blot - Anti-LBP antibody [EPR10865] (ab169776) at 1/1000 dilution

Lane 1:

Human serum lysate at 10 µg

Lane 2:

Human plasma lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 53 kDa

false

• WB

CiteAb

Western blot - Anti-LBP antibody [EPR10865] (AB169776)

LBP western blot using anti-LBP antibody [EPR10865] ab169776. Publication image and figure legend from Huang, X., Zeng, Y., et al., 2017, Mediators Inflamm, PubMed 28634422.

ab169776 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab169776 please see the product overview.

LBP gene overexpression further decreased LPS-induced downregulation of FKN mRNA and protein expression; LBP gene silencing, SB203580, and SC-514 suppressed LPS-induced downregulation of FKN mRNA and protein expression, respectively, in A549 cells. RT-PCR and ELISA were used to analyze the LBP and FKN mRNA and protein expression, respectively, in A549 cells. The LBP mRNA expression is shown in (a). The relative LBP mRNA levels (normalized to GAPDH mRNA) in LPS-stimulated cells are significantly higher than those in control cells (p < 0.05). The LBP mRNA levels of the LBP(+) group and LBP(-) group are upregulated and downregulated, respectively, when compared with the control group (p < 0.05 in all cases). The LBP mRNA levels of the LPS+LBP(+) group of cells are upregulated compared with those of the control group and LPS group (p < 0.05 in all cases). The LBP mRNA levels of the LPS+LBP(-) group of cells are upregulated compared with those of the control group and downregulated compared with those of the LPS group (p < 0.05 in all cases). The LBP mRNA level of the empty vector group is not different from that of the control group (p > 0.05). The FKN mRNA expression is shown in (e). The relative FKN mRNA levels (normalized to GAPDH mRNA) in LPS-stimulated cells are significantly lower than those in control cells (p < 0.05). The FKN mRNA levels of the LPS+LBP(+) group of cells are downregulated compared with those of the control group and the LPS group (p < 0.05 in all cases). The FKN mRNA levels of the LPS+LBP(-), LPS+SB, and LPS+SC groups of cells are downregulated compared with those of the control group and upregulated compared with those of the LPS group (p < 0.05 in all cases). The FKN mRNA levels of the TNF-α group are upregulated compared with those of the control group (p < 0.05). The results of ELISA and western blot in (b), (c), and (d) demonstrate that the LPS treatment significantly increases the levels of LBP protein expression (p < 0.05). The LBP protein levels of the LBP(+) group and LBP(-) group are upregulated and downregulated, respectively, compared with those of the control group (p < 0.05 in all cases). The LBP protein levels of the LPS+LBP group of cells are upregulated compared with those of the control group and the LPS group (p < 0.05 in all cases). The LBP protein levels of the LPS+LBP(-) group of cells are upregulated compared with those of the control group and downregulated compared with those of the LPS group (p < 0.05 in all cases). ELISA and western blot assay in (f), (g), and (h) demonstrate that the LPS treatment significantly reduces the levels of FKN protein expression (p < 0.05). The FKN protein levels of the LPS+LBP group of cells are downregulated compared with those of the control group and the LPS group (p < 0.05 in all cases). The FKN protein levels of the LPS+LBP(-), LPS+SB, and LPS+SC groups of cells are downregulated compared with those of the control group and upregulated compared with those of the LPS group (p < 0.05 in all cases). The FKN protein levels of the TNF-α group are higher than those of the control group (p < 0.05). Immunofluorescence staining results in (i) and (j) confirmed that TNF-α induced a high FKN-positive cell ratio in A549 cells. LPS decreased FKN-positive cell ratio in A549 cells; LBP gene overexpression further decreased FKN-positive cell ratio which was inhibited by LPS, LBP gene silencing, and SB203580. Also, SC-514 reduced the inhibition of LPS (p < 0.05 in all cases). The photomicrographs are 200x magnification. Data are presented as means ± SD of six independent experiments (n = 6). * represents p < 0.05 when compared with that in the CTL; # represents p < 0.05 when compared with that in the LPS; △ represents p < 0.05 when compared with that in the LPS+LBP(+). *p < 0.05 when compared with that in the control group; ◇ represents p < 0.05 when compared with that in the LPS+LBP(-) group; △ represents p < 0.05 when compared with that in the LPS+LBP(+).

false