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Rabbit Recombinant Monoclonal LBP antibody. Suitable for IP, WB and reacts with Mouse samples. Cited in 2 publications.

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Images

Immunoprecipitation - Anti-LBP antibody [EPR22559-261] (AB254559), expandable thumbnail
  • Western blot - Anti-LBP antibody [EPR22559-261] (AB254559), expandable thumbnail
  • Western blot - Anti-LBP antibody [EPR22559-261] (AB254559), expandable thumbnail
  • Western blot - Anti-LBP antibody [EPR22559-261] (AB254559), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PIHC-Fr
Mouse
Tested
Tested
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria (PubMed:9144073). Acts as an affinity enhancer for CD14, facilitating its association with LPS (By similarity). Promotes the release of cytokines in response to bacterial lipopolysaccharide (PubMed:24380872).

Alternative names

Lipopolysaccharide-binding protein, LBP, Lbp

Recommended products

Rabbit Recombinant Monoclonal LBP antibody. Suitable for IP, WB and reacts with Mouse samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22559-261
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Lipopolysaccharide-binding protein (LBP) also known as LPS-binding protein is an acute-phase protein that plays a critical role in the immune response to Gram-negative bacterial infections. This protein weighing approximately 60kDa is primarily expressed in the liver and found in the serum. LBP binds to lipopolysaccharides (LPS) which are components of the outer membrane of Gram-negative bacteria facilitating the transfer of LPS to CD14 receptors on immune cells. This transfer enhances the recognition of bacterial endotoxins triggering an immune response.

Biological function summary

LBP serves a significant role in enhancing the sensitivity of the immune system to bacterial endotoxins. It does not form a permanent part of a larger complex but works closely with other molecules like CD14 and MD-2 an accessory protein of the TLR4 receptor to mediate the innate immune response. By efficiently binding LPS LBP aids in transporting these molecules to cell surface receptors accelerating the detection and subsequent response to infections.

Pathways

LBP is heavily involved in the TLR4 signaling pathway which is important for the detection and response to Gram-negative bacterial infections. Through this pathway LBP and associated proteins such as CD14 and MD-2 help activate TLR4 triggering downstream NF-κB signaling. This activation leads to the production of pro-inflammatory cytokines and an upsurge in antimicrobial activities fortifying the body's defenses against bacterial invasion.

Associated diseases and disorders

Enhanced LBP levels have been associated with sepsis a severe systemic response to bacterial infections which can often lead to septic shock. Given its pivotal role in recognizing bacterial endotoxins LBP is important in identifying infections making it a potential prospect for therapeutic targeting. Additionally its interaction with CD14 means that alterations in LBP function or expression levels might contribute to immune dysregulation seen in inflammatory diseases such as Crohn's disease where immune responses to gut microbiota play a central role.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunoprecipitation - Anti-LBP antibody [EPR22559-261] (ab254559), expandable thumbnail

    Immunoprecipitation - Anti-LBP antibody [EPR22559-261] (ab254559)

    LBP was immunoprecipitated from 0.35 mg mouse ovary lysate with ab254559 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab254559 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used as the secondary antibody at 1/5000 dilution.

    Lane 1: Mouse ovary lysate 10μg (Input).
    Lane 2: ab254559 IP in mouse ovary lysate.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab254559 in mouse ovary lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 20 seconds.

    This blot was developed using a higher sensitivity ECL substrate.

    All lanes: Immunoprecipitation - Anti-LBP antibody [EPR22559-261] (ab254559)

    Predicted band size: 53 kDa

    Observed band size: 60 kDa

  • Western blot - Anti-LBP antibody [EPR22559-261] (ab254559), expandable thumbnail

    Western blot - Anti-LBP antibody [EPR22559-261] (ab254559)

    Blocking/diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 59 seconds

    The expression profile / molecular weight observed is consistent with what has been described in the literature (PMID:7694297).

    All lanes: Western blot - Anti-LBP antibody [EPR22559-261] (ab254559) at 1/1000 dilution

    Lane 1: Mouse serum at 20 µg

    Lane 2: Mouse plasma at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 53 kDa

    Observed band size: 60 kDa

  • Western blot - Anti-LBP antibody [EPR22559-261] (ab254559), expandable thumbnail

    Western blot - Anti-LBP antibody [EPR22559-261] (ab254559)

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 59 seconds

    The expression profile / molecular weight observed is consistent with what has been described in the literature (PMID:7694297)

    Negative control: SP2/0-Ag14 (PMID: 2456339).

    All lanes: Western blot - Anti-LBP antibody [EPR22559-261] (ab254559) at 1/1000 dilution

    Lane 1: SP2/0-Ag14 (mouse myeloma cell line), whole cell lysate at 20 µg

    Lane 2: Mouse ovary tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 53 kDa

    Observed band size: 60 kDa

  • Western blot - Anti-LBP antibody [EPR22559-261] (ab254559), expandable thumbnail

    Western blot - Anti-LBP antibody [EPR22559-261] (ab254559)

    Blocking/diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    All lanes: Western blot - Anti-LBP antibody [EPR22559-261] (ab254559) at 1/1000 dilution

    All lanes: His-tagged full-length recombinant mouse LBP protein 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa

    Observed band size: 60 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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