AB232940

Anti-LC3B antibody [EPR21234]

Name: Anti-LC3B antibody [EPR21234] (ab232940) | Abcam
Brand: Abcam Limited
SKU: AB232940
Price: 400 USD
Availability: InStock
Rating: 4 (4 reviews)

Recombinant
KO Validated
Lab Essentials
20ul selling size
What is this?

(4 Reviews)

(20 Publications)

Rabbit Recombinant Monoclonal LC3B antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 20 publications.

View Alternative Names

MAP1ALC3, MAP1LC3B, Microtubule-associated protein 1 light chain 3 beta, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, Microtubule-associated proteins 1A/1B light chain 3B, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody [EPR21234] (AB232940)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody [EPR21234] (AB232940)

ab232940 staining LC3B in HAP1 cells (wildtype and MAP1LC3B knockout) +/- Chloroquine (50μM, 24 hours). The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab232940 at 0.1μg/ml and ab195889 then detected with an Alexa Fluor^® 488 goat anti-rabbit secondary antibody (ab150081) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at a 1/250 dilution (shown in red).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21234

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Product details

This product was made using synthetic libraries and phage display technology.

This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LC3B also known as microtubule-associated protein 1 light chain 3 beta plays an important role in autophagy a process that maintains cellular homeostasis. The LC3B protein with a molecular weight of approximately 14 kDa undergoes lipidation to form LC3-II which associates with autophagosomal membranes. This protein expresses ubiquitously in various tissues including liver muscle and brain. Researchers often detect LC3B using techniques like LC3B western blot and LC3B immunofluorescence due to its function as a marker indicating autophagy levels.

Biological function summary

LC3B contributes significantly to the formation and maturation of autophagosomes. LC3B part of the autophagy-related protein complex binds to autophagic membranes. During this process LC3-I converts to LC3-II a lipid-phosphatidylethanolamine conjugate essential for autophagosome membrane expansion and closure. This mechanism helps remove damaged organelles and misfolded proteins from cells therefore contributing to cellular quality control.

Pathways

LC3B integrates into the autophagy pathway which is critical for cellular adaptive responses to stress. The mammalian target of rapamycin (mTOR) pathway regulates autophagy where mTOR inhibition activates LC3B promoting autophagosome formation. Moreover LC3B operates alongside other proteins like Beclin-1 and ULK1 facilitating the initiation and progression of autophagy under nutrient starvation conditions. These interactions highlight LC3's role in cellular energy balance and survival mechanisms.

LC3B connects with conditions such as cancer and neurodegenerative diseases. Altered autophagy levels mediated by LC3B often associate with tumorigenesis where its dysregulation can affect cancer progression. Furthermore LC3B also links to neurodegenerative diseases like Alzheimer's where impaired autophagy disrupts neuronal function. LC3B interacts with proteins such as p62/SQSTM1 which affects protein aggregate clearance a critical factor in neurodegenerative pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed : 20418806, PubMed : 23209295, PubMed : 28017329). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production (PubMed : 23209295, PubMed : 28017329). In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria (PubMed : 22922758). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed : 20418806, PubMed : 23209295, PubMed : 28017329). Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway (PubMed : 24089205). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed : 31006537, PubMed : 31006538). Upon nutrient stress, directly recruits cofactor JMY to the phagophore membrane surfaces and promotes JMY's actin nucleation activity and autophagosome biogenesis during autophagy (PubMed : 30420355).

See full target information MAP1LC3B

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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Discover oncology 16:1789 PubMed41026320

2025

CDCA5 knockdown potentiates olaparib sensitivity in BRCA1-mutated ovarian cancer through autophagy activation.

Applications

Unspecified application

Species

Unspecified reactive species

Wenzong Wu,Huan Wu,Yuqing Song,LiuJun Wu,Jiajia Chen,Minsheng Zhu,Xinchun Wu,Jiawei Wang,Qingsong Zhang

PloS one 20:e0329352 PubMed40768414

2025

Quantification of H3.1-nucleosomes using a chemiluminescent immunoassay: A reliable method for neutrophil extracellular trap detection.

Applications

Unspecified application

Species

Unspecified reactive species

Marion Wargnies,Guillaume Rommelaere,Julie Candiracci,Dorian Pamart,Robin Varsebroucq,Florian Jibassia,Finley Serneo,Virginie Laloux,Olivia Thiry,Fanny Lambert,Alison Lobbens,Priscilla Van den Ackerveken,Marielle Herzog

European journal of histochemistry : EJH 69: PubMed40586548

2025

NR4A3 suppresses bladder cancer progression by modulating autophagy the PI3K/AKT/mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Li Fan,Feng Xu,Shouyong Liu,Ding Wu,Suchun Wang,Xin Pan,Yulin Zhou,Le Qu,Wenquan Zhou

Discover oncology 16:687 PubMed40338396

2025

Inhibition of ATG5-mediated autophagy maintains PMAIP1 stability to promote cell apoptosis and suppress triple-negative breast cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Fangjian Shang,Hongfeng Nie,Liyan Du,Jin Shang,Xiangquan Song,Ya Chen,Hui Li,Zhuo Wang,Yixin Qi,Liyan Zhao

Journal of oral & facial pain and headache 39:196-203 PubMed40129438

2025

Loss of SCRG1 in chondrocytes inhibits osteoarthritis by promoting autophagy activity in the temporomandibular joint through inhibition of neurokine receptors.

Applications

Unspecified application

Species

Unspecified reactive species

JiaJun Zhang,LePing Yuan,YanYan Zhang,HaoYang Jin,YeKe Zhao,XiaoKe Zeng,YanHui Zou,KeYu Wang,Xin Nie

Histology and histopathology 40:1651-1665 PubMed40008600

2025

Dian sanguis draconis improves pulmonary fibrosis by activating autophagy to regulate the PA/PAI-1 balance.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayi Song,Qian Li,Chunyan Yang,Qing Liu,Jianmei Li,Yi Fu

Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 33:e13253 PubMed39823159

2025

Bacteria in hypertrophic scars promote scar formation through HSBP1-mediated autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Yuan,Jiarong Yu,Jiaoyun Dong,Zhigang Mao,Xiqiao Wang

Experimental and therapeutic medicine 28:402 PubMed39234585

2024

PIN1‑silencing mitigates keratinocyte proliferation and the inflammatory response in psoriasis by activating mitochondrial autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Xia,Jin Li,Hongshan Yuan,Wenliang Yan

PeerJ 12:e17263 PubMed38784395

2024

PTX3 mediates PI3K/AKT/mTOR signaling to downregulate apoptosis and autophagy to attenuate myocardial injury in sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Na Cui,Zhi Chen,Zhanbiao Yu,Xiaowei Lv,Zhenjie Hu

International journal of oral science 16:35 PubMed38719825

2024

Phenformin activates ER stress to promote autophagic cell death via NIBAN1 and DDIT4 in oral squamous cell carcinoma independent of AMPK.

Applications

Unspecified application

Species

Unspecified reactive species

Dexuan Zhuang,Shuangshuang Wang,Huiting Deng,Yuxin Shi,Chang Liu,Xue Leng,Qun Zhang,Fuxiang Bai,Bin Zheng,Jing Guo,Xunwei Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LC3B antibody [EPR21234]

Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody [EPR21234] (AB232940)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (20)

Product promise