AB239416

Anti-LC3B antibody [EPR21235]

Name: Anti-LC3B antibody [EPR21235] (ab239416) | Abcam
Brand: Abcam Limited
SKU: AB239416
Price: 135 USD
Availability: InStock
Rating: 1 (1 reviews)

Recombinant
KO Validated
Lab Essentials
20ul selling size
What is this?

(1 Review)

(9 Publications)

Rabbit Recombinant Monoclonal LC3B antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 9 publications.

View Alternative Names

MAP1ALC3, MAP1LC3B, Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, Microtubule-associated protein 1 light chain 3 beta, MAP1A/MAP1B LC3 B

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody [EPR21235] (AB239416)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody [EPR21235] (AB239416)

ab239416 staining LC3B in HAP1 cells (wildtype and MAP1LC3B knockout) +/- Chloroquine (50μM, 24 hours). The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab239416 at 0.1μg/ml and ab195889 then detected with an Alexa Fluor^® 488 goat anti-rabbit secondary antibody (ab150081) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at a 1/250 dilution (shown in red).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21235

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.1 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Product details

This product was made using synthetic libraries and phage display technology.

This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LC3B also known as microtubule-associated protein 1 light chain 3 beta plays an important role in autophagy a process that maintains cellular homeostasis. The LC3B protein with a molecular weight of approximately 14 kDa undergoes lipidation to form LC3-II which associates with autophagosomal membranes. This protein expresses ubiquitously in various tissues including liver muscle and brain. Researchers often detect LC3B using techniques like LC3B western blot and LC3B immunofluorescence due to its function as a marker indicating autophagy levels.

Biological function summary

LC3B contributes significantly to the formation and maturation of autophagosomes. LC3B part of the autophagy-related protein complex binds to autophagic membranes. During this process LC3-I converts to LC3-II a lipid-phosphatidylethanolamine conjugate essential for autophagosome membrane expansion and closure. This mechanism helps remove damaged organelles and misfolded proteins from cells therefore contributing to cellular quality control.

Pathways

LC3B integrates into the autophagy pathway which is critical for cellular adaptive responses to stress. The mammalian target of rapamycin (mTOR) pathway regulates autophagy where mTOR inhibition activates LC3B promoting autophagosome formation. Moreover LC3B operates alongside other proteins like Beclin-1 and ULK1 facilitating the initiation and progression of autophagy under nutrient starvation conditions. These interactions highlight LC3's role in cellular energy balance and survival mechanisms.

LC3B connects with conditions such as cancer and neurodegenerative diseases. Altered autophagy levels mediated by LC3B often associate with tumorigenesis where its dysregulation can affect cancer progression. Furthermore LC3B also links to neurodegenerative diseases like Alzheimer's where impaired autophagy disrupts neuronal function. LC3B interacts with proteins such as p62/SQSTM1 which affects protein aggregate clearance a critical factor in neurodegenerative pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed : 20418806, PubMed : 23209295, PubMed : 28017329). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production (PubMed : 23209295, PubMed : 28017329). In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria (PubMed : 22922758). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed : 20418806, PubMed : 23209295, PubMed : 28017329). Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway (PubMed : 24089205). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed : 31006537, PubMed : 31006538). Upon nutrient stress, directly recruits cofactor JMY to the phagophore membrane surfaces and promotes JMY's actin nucleation activity and autophagosome biogenesis during autophagy (PubMed : 30420355).

See full target information MAP1LC3B

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

International journal of veterinary science and medicine 13:1-9 PubMed40290667

2025

Post-operative supplementation with vitamin D after mucogingival surgery significantly enhances autophagy and improves life quality following feline chronic gingivostomatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yung-Kai Huang,Chung-Wei Chen,Ting-Yi Renn,Ming-Yi Shen,Igor V Klimenkov,Nikolay P Sudakov,Sandeep Kumar Singh,Hung-Ming Chang

Aging 16:3763-3772 PubMed38364258

2024

Liraglutide improves sevoflurane-induced postoperative cognitive dysfunction via activating autophagy and inhibiting apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Hu,Haijin Huang,Yao Jiang,Jingling Zhang,Yang Zhang,Ying Tian,Qin Zhang

Experimental and therapeutic medicine 25:247 PubMed37153895

2023

Knockdown of KAT5/KIF11 induces autophagy and promotes apoptosis in anaplastic thyroid cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Fu,Lina Zhao,Chunyan Liao,Pengzhen Wang,Ying Gu,Sha Li,Liying Shi,Qiaorong Wang,Jin Xie,Lixin Zhang,Xiaoming Liu,Bei Zhang

Food science & nutrition 11:1965-1981 PubMed37051356

2023

lncRNA TINCR knockdown inhibits colon cancer cells via regulation of autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jianhua Xu,Wenge Zeng,Tiantian Liu,Zhenda Wan,Xin Yang,Jun Chen,Fei Liu

Computational and mathematical methods in medicine 2022:6260202 PubMed36193200

2022

Dapagliflozin Inhibits Ventricular Remodeling in Heart Failure Rats by Activating Autophagy through AMPK/mTOR Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Honghong Ma,Yanmei Ma

Oxidative medicine and cellular longevity 2022:2815187 PubMed36035205

2022

HDAC8 Promotes Liver Metastasis of Colorectal Cancer Inhibition of IRF1 and Upregulation of SUCNR1.

Applications

Unspecified application

Species

Unspecified reactive species

Jierong Chen,Lixue Cao,Jianhong Ma,Caifeng Yue,Dandan Zhu,Ran An,Xiaoxiao Wang,Yunmiao Guo,Bing Gu

iScience 25:105029 PubMed36111256

2022

MCM7 supports the stemness of bladder cancer stem-like cells by enhancing autophagic flux.

Applications

Unspecified application

Species

Unspecified reactive species

Lijun Mo,Bijia Su,Lili Xu,Zhiming Hu,Hongwei Li,Hongyan Du,Jinlong Li

Synapse (New York, N.Y.) 75:e22221 PubMed34958692

2021

Role of Stat3 in NLRP3/caspase-1-mediated hippocampal neuronal pyroptosis in epileptic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Jiang,Guo Tang,Xue-Min Zhong,Dan-Rui Ding,Hui Wang,Jia-Ni Li

Bioengineered 12:10187-10198 PubMed34860641

2021

Baicalin suppresses glaucoma pathogenesis by regulating the PI3K/AKT signaling in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Ningmin Zhao,Jieran Shi,Haohang Xu,Qing Luo,Qiaoyan Li,Mingzhou Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LC3B antibody [EPR21235]

Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody [EPR21235] (AB239416)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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