AB243506

Anti-LC3B antibody [GT3612]

Name: Anti-LC3B antibody [GT3612] (ab243506) | Abcam
Brand: Abcam Limited
SKU: AB243506
Price: 400 USD
Availability: InStock
Rating: 1 (1 reviews)

Lab Essentials
What is this?

(1 Review)

(11 Publications)

Mouse Monoclonal LC3B antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human MAP1LC3B.

View Alternative Names

MAP1ALC3, MAP1LC3B, Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, Microtubule-associated protein 1 light chain 3 beta, MAP1A/MAP1B LC3 B

4 Images

Supplier Data

Western blot - Anti-LC3B antibody [GT3612] (AB243506)

15% SDS-PAGE gel.

All lanes:

Western blot - Anti-LC3B antibody [GT3612] (ab243506) at 1/500 dilution

Lane 1:

Untreated HepG2 (human liver hepatocellular carcinoma cell line) whole cell extracts at 30 µg

Lane 2:

3 μM Thapsigargin (12 hr) treated HepG2 whole cell extracts at 30 µg

Predicted band size: 15 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody [GT3612] (AB243506)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody [GT3612] (AB243506)

4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells, mock treated (left) and treated with 50 μM Chloroquine for 24 hr (right), stained for LC3B (green) using ab243506 at 1/200 dilution in ICC/IF.

Blue : Hoechst 33342 staining.

Scale bar = 10 μm.

Unknown

Western blot - Anti-LC3B antibody [GT3612] (AB243506)

15% SDS-PAGE gel.

All lanes:

Western blot - Anti-LC3B antibody [GT3612] (ab243506) at 1/500 dilution

Lane 1:

Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts at 50 µg

Lane 2:

50μM Chloroquine (24 hr) treated HeLa whole cell extracts at 50 µg

Predicted band size: 15 kDa

false

Supplier Data

Western blot - Anti-LC3B antibody [GT3612] (AB243506)

15% SDS-PAGE gel.

All lanes:

Western blot - Anti-LC3B antibody [GT3612] (ab243506) at 1/500 dilution

Lane 1:

Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts at 30 µg

Lane 2:

LC3A-transfected HEK-293T whole cell extracts at 30 µg

Lane 3:

LC3B-transfected HEK-293T whole cell extracts at 30 µg

Predicted band size: 15 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

GT3612

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human MAP1LC3B. The exact immunogen used to generate this antibody is proprietary information.

Q9GZQ8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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AB139484

Autophagy Assay Kit

2 Reviews

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Why is this recommended?

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: 100% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LC3B also known as microtubule-associated protein 1 light chain 3 beta plays an important role in autophagy a process that maintains cellular homeostasis. The LC3B protein with a molecular weight of approximately 14 kDa undergoes lipidation to form LC3-II which associates with autophagosomal membranes. This protein expresses ubiquitously in various tissues including liver muscle and brain. Researchers often detect LC3B using techniques like LC3B western blot and LC3B immunofluorescence due to its function as a marker indicating autophagy levels.

Biological function summary

LC3B contributes significantly to the formation and maturation of autophagosomes. LC3B part of the autophagy-related protein complex binds to autophagic membranes. During this process LC3-I converts to LC3-II a lipid-phosphatidylethanolamine conjugate essential for autophagosome membrane expansion and closure. This mechanism helps remove damaged organelles and misfolded proteins from cells therefore contributing to cellular quality control.

Pathways

LC3B integrates into the autophagy pathway which is critical for cellular adaptive responses to stress. The mammalian target of rapamycin (mTOR) pathway regulates autophagy where mTOR inhibition activates LC3B promoting autophagosome formation. Moreover LC3B operates alongside other proteins like Beclin-1 and ULK1 facilitating the initiation and progression of autophagy under nutrient starvation conditions. These interactions highlight LC3's role in cellular energy balance and survival mechanisms.

LC3B connects with conditions such as cancer and neurodegenerative diseases. Altered autophagy levels mediated by LC3B often associate with tumorigenesis where its dysregulation can affect cancer progression. Furthermore LC3B also links to neurodegenerative diseases like Alzheimer's where impaired autophagy disrupts neuronal function. LC3B interacts with proteins such as p62/SQSTM1 which affects protein aggregate clearance a critical factor in neurodegenerative pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed : 20418806, PubMed : 23209295, PubMed : 28017329). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production (PubMed : 23209295, PubMed : 28017329). In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria (PubMed : 22922758). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed : 20418806, PubMed : 23209295, PubMed : 28017329). Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway (PubMed : 24089205). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed : 31006537, PubMed : 31006538). Upon nutrient stress, directly recruits cofactor JMY to the phagophore membrane surfaces and promotes JMY's actin nucleation activity and autophagosome biogenesis during autophagy (PubMed : 30420355).

See full target information MAP1LC3B

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

BMC musculoskeletal disorders 23:398 PubMed35484550

2022

Low-frequency electrical stimulation alleviates immobilization-evoked disuse muscle atrophy by repressing autophagy in skeletal muscle of rabbits.

Applications

Unspecified application

Species

Unspecified reactive species

A-Ying Liu,Quan-Bing Zhang,Hua-Long Zhu,Yong-Wei Xiong,Feng Wang,Peng-Peng Huang,Qi-Yu Xu,Hua-Zhang Zhong,Hua Wang,Yun Zhou

Frontiers in cardiovascular medicine 8:768214 PubMed35083298

2022

Sodium-Glucose Co-transporter-2 Inhibitor of Dapagliflozin Attenuates Myocardial Ischemia/Reperfusion Injury by Limiting NLRP3 Inflammasome Activation and Modulating Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yong-Wei Yu,Jia-Qun Que,Shuai Liu,Kai-Yu Huang,Lu Qian,Ying-Bei Weng,Fang-Ning Rong,Lei Wang,Ying-Ying Zhou,Yang-Jing Xue,Kang-Ting Ji

BMC neuroscience 23:3 PubMed34983390

2022

Increased expression of pathological markers in Parkinson's disease dementia post-mortem brains compared to dementia with Lewy bodies.

Applications

Unspecified application

Species

Unspecified reactive species

Haitao Tu,Zhi Wei Zhang,Lifeng Qiu,Yuning Lin,Mei Jiang,Sook-Yoong Chia,Yanfei Wei,Adeline S L Ng,Richard Reynolds,Eng-King Tan,Li Zeng

Journal of inflammation (London, England) 18:31 PubMed34930287

2021

Ginsenoside Rg3 alleviates septic liver injury by regulating the lncRNA TUG1/miR-200c-3p/SIRT1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Pan Wu,Xiao Yu,Yue Peng,Qian-Lu Wang,Long-Tian Deng,Wei Xing

Autophagy 18:1433-1449 PubMed34740307

2021

Extracellular vesicles originating from autophagy mediate an antibody-resistant spread of classical swine fever virus in cell culture.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Wang,Liang Zhang,Wulong Liang,Shanchuan Liu,Wen Deng,Yangruiyu Liu,Yaru Liu,Mengzhao Song,Kangkang Guo,Yanming Zhang

Autophagy 18:1534-1550 PubMed34689707

2021

Human platelets display dysregulated sepsis-associated autophagy, induced by altered LC3 protein-protein interaction of the Vici-protein EPG5.

Applications

Unspecified application

Species

Unspecified reactive species

Hansjörg Schwertz,Jesse W Rowley,Irina Portier,Elizabeth A Middleton,Neal D Tolley,Robert A Campbell,Alicia S Eustes,Karin Chen,Matthew T Rondina

Scientific reports 11:19417 PubMed34593893

2021

Defined D-hexapeptides bind CUG repeats and rescue phenotypes of myotonic dystrophy myotubes in a Drosophila model of the disease.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Rapisarda,Ariadna Bargiela,Beatriz Llamusi,Isabel Pont,Roger Estrada-Tejedor,Enrique Garcia-España,Ruben Artero,Manuel Perez-Alonso

Oncology letters 21:470 PubMed33907580

2021

LACTB and LC3 could serve as potential biomarkers of gastric cancer to neoadjuvant chemotherapy with oxaliplatin plus S-1.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Yang,Zhiqiang Yan,Wei Nie,Zeying Liu,Xingzhen Cheng,Wei Wang,Chunyan Shao,Gui Fu,Yanni Yu

Molecular medicine reports 22:2219-2226 PubMed32705226

2020

Suppression of sirtuin 1 alleviates airway inflammation through mTOR‑mediated autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Wu,Wei Li,Yifan Hu,Yun Liu,Xiuzhen Sun

Journal of cellular and molecular medicine 24:8636-8649 PubMed32596952

2020

HDAC1-mediated deacetylation of LSD1 regulates vascular calcification by promoting autophagy in chronic renal failure.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajun Zhou,Han Zhou,Caixin Liu,Lin Huang,Dongmei Lu,Chaoqing Gao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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alexa-fluor-488-lc3b-antibody-epr18709-autophagosome-marker-ab225382

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