AB222776

Anti-LC3B antibody [RM293]

Recombinant
Lab Essentials
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(6 Publications)

Rabbit Recombinant Monoclonal LC3B antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human MAP1LC3B.

View Alternative Names

MAP1ALC3, MAP1LC3B, Microtubule-associated protein 1 light chain 3 beta, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, Microtubule-associated proteins 1A/1B light chain 3B, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody [RM293] (AB222776)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody [RM293] (AB222776)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells, untreated (left panel) or treated with chloroquine (right panel) stained for LC3B (red) using ab222776 at 1/200 dilution in ICC/IF.

Actin filaments were labeled with fluorescein phalloidin (green). Nuclei were labeled with DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LC3B antibody [RM293] (AB222776)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LC3B antibody [RM293] (AB222776)

Formalin-fixed, paraffin-embedded human brain tissue stained for LC3B with ab222776 at 1/200 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-LC3B antibody [RM293] (AB222776)

All lanes:

Western blot - Anti-LC3B antibody [RM293] (ab222776) at 1/200 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) treated with chloroquine, cell lysate

Predicted band size: 15 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM293

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human MAP1LC3B. The exact immunogen used to generate this antibody is proprietary information.

Q9GZQ8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/200", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

ab222776 was purified from an animal origin–free culture supernatant.

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LC3B also known as microtubule-associated protein 1 light chain 3 beta plays an important role in autophagy a process that maintains cellular homeostasis. The LC3B protein with a molecular weight of approximately 14 kDa undergoes lipidation to form LC3-II which associates with autophagosomal membranes. This protein expresses ubiquitously in various tissues including liver muscle and brain. Researchers often detect LC3B using techniques like LC3B western blot and LC3B immunofluorescence due to its function as a marker indicating autophagy levels.

Biological function summary

LC3B contributes significantly to the formation and maturation of autophagosomes. LC3B part of the autophagy-related protein complex binds to autophagic membranes. During this process LC3-I converts to LC3-II a lipid-phosphatidylethanolamine conjugate essential for autophagosome membrane expansion and closure. This mechanism helps remove damaged organelles and misfolded proteins from cells therefore contributing to cellular quality control.

Pathways

LC3B integrates into the autophagy pathway which is critical for cellular adaptive responses to stress. The mammalian target of rapamycin (mTOR) pathway regulates autophagy where mTOR inhibition activates LC3B promoting autophagosome formation. Moreover LC3B operates alongside other proteins like Beclin-1 and ULK1 facilitating the initiation and progression of autophagy under nutrient starvation conditions. These interactions highlight LC3's role in cellular energy balance and survival mechanisms.

LC3B connects with conditions such as cancer and neurodegenerative diseases. Altered autophagy levels mediated by LC3B often associate with tumorigenesis where its dysregulation can affect cancer progression. Furthermore LC3B also links to neurodegenerative diseases like Alzheimer's where impaired autophagy disrupts neuronal function. LC3B interacts with proteins such as p62/SQSTM1 which affects protein aggregate clearance a critical factor in neurodegenerative pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed : 20418806, PubMed : 23209295, PubMed : 28017329). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production (PubMed : 23209295, PubMed : 28017329). In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria (PubMed : 22922758). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed : 20418806, PubMed : 23209295, PubMed : 28017329). Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway (PubMed : 24089205). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed : 31006537, PubMed : 31006538). Upon nutrient stress, directly recruits cofactor JMY to the phagophore membrane surfaces and promotes JMY's actin nucleation activity and autophagosome biogenesis during autophagy (PubMed : 30420355).

See full target information MAP1LC3B

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Open life sciences 20:20220913 PubMed40226364

2025

Stigmasterol alleviates endplate chondrocyte degeneration through inducing mitophagy by enhancing PINK1 mRNA acetylation via the ESR1/NAT10 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Li,Xiaofeng Chen,Baoci Huang,Junjie He,Junxian Xie,Weijun Guo,Jinjun Liang,Jiajian Ruan,Jincheng Liu,Zhen Xiang,Lixin Zhu

Cancer biology & therapy 26:2469927 PubMed40018990

2025

The RNA-binding protein ELAVL1 promotes Beclin1-mediated cellular autophagy and thus endometrial cancer development by affecting LncRNA-neat stability.

Applications

Unspecified application

Species

Unspecified reactive species

Yanlu Luo,Xueyan Zhong,Xinzhao Sun,Jiangtao Fan

Oral diseases 29:2837-2844 PubMed35770313

2022

Apocynin inhibits compressive force-induced apoptosis and autophagy of periodontal ligament stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Li,Yuchun Shi,Yanbin Fu

Experimental and therapeutic medicine 22:1271 PubMed34594408

2021

TFEC contributes to cardiac hypertrophy by inhibiting AMPK/mTOR signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Zhao,Zhenyu Wang,Yehong Chi,Chunmei Ni,Xudan Zheng

Molecular medicine reports 24: PubMed34109426

2021

Dexmedetomidine attenuates neuronal injury induced by cerebral ischemia‑reperfusion by regulating miR‑199a.

Applications

Unspecified application

Species

Unspecified reactive species

Yulin Zhu,Huatang Zhao,Wenshan Zhang,Xingang Ma,Ye Liu

Aging 12: PubMed33495420

2021

Hsa_circ_0000199 facilitates chemo-tolerance of triple-negative breast cancer by interfering with miR-206/613-led PI3K/Akt/mTOR signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hongchang Li,Wen Xu,Zhihua Xia,Weiyan Liu,Gaofeng Pan,Junbin Ding,Jindong Li,Jianfa Wang,Xiaofeng Xie,Daowen Jiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LC3B antibody [RM293]

Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody [RM293] (AB222776)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LC3B antibody [RM293] (AB222776)

Western blot - Anti-LC3B antibody [RM293] (AB222776)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise