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AB244212

Anti-LC3C antibody

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Rabbit Polyclonal LC3C antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human MAP1LC3C aa 1-50.

View Alternative Names

Microtubule-associated proteins 1A/1B light chain 3C, Autophagy-related protein LC3 C, Autophagy-related ubiquitin-like modifier LC3 C, MAP1 light chain 3-like protein 3, MAP1A/MAP1B light chain 3 C, Microtubule-associated protein 1 light chain 3 gamma, MAP1A/MAP1B LC3 C, MAP1LC3C

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-LC3C antibody (AB244212)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LC3C antibody (AB244212)

Cytospin-isolated human white blood cells labeling LC3C using ab244212 at a 1/200 dilutiom for 1 hr (red) in ICC/IF.

Myeloperoxidase is green, Hoechst dye is blue.

Immunocytochemistry/ Immunofluorescence - Anti-LC3C antibody (AB244212)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LC3C antibody (AB244212)

Cytospin-isolated human white blood cells labeling LC3C using ab244212 at a 1/200 dilutiom for 1 hr (red) in ICC/IF.

Myeloperoxidase is green, Hoechst dye is blue.

Immunocytochemistry/ Immunofluorescence - Anti-LC3C antibody (AB244212)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LC3C antibody (AB244212)

Cytospin-isolated human white blood cells labeling LC3C using ab244212 at a 1/200 dilutiom for 1 hr (red) in ICC/IF.

Myeloperoxidase is green, Hoechst dye is blue.

Immunocytochemistry/ Immunofluorescence - Anti-LC3C antibody (AB244212)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LC3C antibody (AB244212)

Cytospin-isolated human white blood cells labeling LC3C using ab244212 at a 1/200 dilutiom for 1 hr (red) in ICC/IF.

Myeloperoxidase is green, Hoechst dye is blue.

Western blot - Anti-LC3C antibody (AB244212)
  • WB

Supplier Data

Western blot - Anti-LC3C antibody (AB244212)

All lanes:

Western blot - Anti-LC3C antibody (ab244212) at 1/100 dilution

All lanes:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Predicted band size: 17 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human MAP1LC3C aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9BXW4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 100µL
Purity
Whole antiserum
Storage buffer
pH: 7 - 7.4
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein LC3C also known as microtubule-associated protein 1 light chain 3 gamma is part of the ATG8 family and is critical in the autophagy pathway. LC3C with a molecular mass of approximately 14.7 kDa participates in the formation of autophagosomes. This protein predominantly localizes in the cytoplasm and transitions to autophagosome membranes during autophagy. LC3C expression occurs in multiple tissues including the brain and liver indicating its broad role in cellular function.
Biological function summary

Microtubule-associated protein 1 light chain 3 gamma acts as an important component in the autophagy process. It is part of a complex that involves other homologs of the LC3 protein family. LC3C interacts directly with phosphatidylethanolamine on autophagosome membranes contributing significantly to the expansion and sealing of these structures. This action is critical for cellular homeostasis and survival during nutrient deprivation.

Pathways

The protein LC3C is an integral part of the macroautophagy pathway. It associates with proteins such as p62 (SQSTM1) which delivers ubiquitinated cargo to autophagosomes. LC3C also interacts with Beclin-1 a protein involved in the initiation of autophagy. These interactions place LC3C within the autophagic machinery essential for lysosomal degradation and recycling of cellular components facilitating proper cellular function.

Research indicates that microtubule-associated protein 1 light chain 3 gamma relates to cancer and neurodegenerative disorders. In cancer altered LC3C levels can affect tumor cell survival and adaptation to stress associating with proteins such as PINK1 which regulates mitochondrial autophagy. In neurodegenerative diseases improper autophagy due to dysfunctional LC3C may contribute to the accumulation of damaged proteins leading to conditions like Parkinson's disease where LC3C interacts with parkin an important player in targeting dysfunctional mitochondria for removal through mitophagy.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ubiquitin-like modifier that plays a crucial role in antibacterial autophagy (xenophagy) through the selective binding of CALCOCO2 (PubMed : 23022382). Recruits all ATG8 family members to infecting bacteria such as S.typhimurium (PubMed : 23022382). May also play a role in aggrephagy, the macroautophagic degradation of ubiquitinated and aggregated proteins (PubMed : 28404643).
See full target information MAP1LC3C
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