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Rabbit Polyclonal LC3C antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human MAP1LC3C aa 1-50.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-LC3C antibody (AB244212), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-LC3C antibody (AB244212), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-LC3C antibody (AB244212), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-LC3C antibody (AB244212), expandable thumbnail
  • Western blot - Anti-LC3C antibody (AB244212), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7 - 7.4

Form

Lyophilized

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human MAP1LC3C aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BXW4

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Ubiquitin-like modifier that plays a crucial role in antibacterial autophagy (xenophagy) through the selective binding of CALCOCO2 (PubMed:23022382). Recruits all ATG8 family members to infecting bacteria such as S.typhimurium (PubMed:23022382). May also play a role in aggrephagy, the macroautophagic degradation of ubiquitinated and aggregated proteins (PubMed:28404643).

Alternative names

Recommended products

Rabbit Polyclonal LC3C antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human MAP1LC3C aa 1-50.

Key facts

Isotype

IgG

Form

Lyophilized

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human MAP1LC3C aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BXW4
Purity

Whole antiserum

Reconstitution

Reconstitute in 100 micro L of sterile water. Centrifuge to remove any insoluble material. Add glycerol 1:1 for added stability for storage.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The protein LC3C also known as microtubule-associated protein 1 light chain 3 gamma is part of the ATG8 family and is critical in the autophagy pathway. LC3C with a molecular mass of approximately 14.7 kDa participates in the formation of autophagosomes. This protein predominantly localizes in the cytoplasm and transitions to autophagosome membranes during autophagy. LC3C expression occurs in multiple tissues including the brain and liver indicating its broad role in cellular function.

Biological function summary

Microtubule-associated protein 1 light chain 3 gamma acts as an important component in the autophagy process. It is part of a complex that involves other homologs of the LC3 protein family. LC3C interacts directly with phosphatidylethanolamine on autophagosome membranes contributing significantly to the expansion and sealing of these structures. This action is critical for cellular homeostasis and survival during nutrient deprivation.

Pathways

The protein LC3C is an integral part of the macroautophagy pathway. It associates with proteins such as p62 (SQSTM1) which delivers ubiquitinated cargo to autophagosomes. LC3C also interacts with Beclin-1 a protein involved in the initiation of autophagy. These interactions place LC3C within the autophagic machinery essential for lysosomal degradation and recycling of cellular components facilitating proper cellular function.

Associated diseases and disorders

Research indicates that microtubule-associated protein 1 light chain 3 gamma relates to cancer and neurodegenerative disorders. In cancer altered LC3C levels can affect tumor cell survival and adaptation to stress associating with proteins such as PINK1 which regulates mitochondrial autophagy. In neurodegenerative diseases improper autophagy due to dysfunctional LC3C may contribute to the accumulation of damaged proteins leading to conditions like Parkinson's disease where LC3C interacts with parkin an important player in targeting dysfunctional mitochondria for removal through mitophagy.

Product promise

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5 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-LC3C antibody (ab244212), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-LC3C antibody (ab244212)

    Cytospin-isolated human white blood cells labeling LC3C using ab244212 at a 1/200 dilutiom for 1 hr (red) in ICC/IF.

    Myeloperoxidase is green, Hoechst dye is blue.

  • Immunocytochemistry/ Immunofluorescence - Anti-LC3C antibody (ab244212), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-LC3C antibody (ab244212)

    Cytospin-isolated human white blood cells labeling LC3C using ab244212 at a 1/200 dilutiom for 1 hr (red) in ICC/IF.

    Myeloperoxidase is green, Hoechst dye is blue.

  • Immunocytochemistry/ Immunofluorescence - Anti-LC3C antibody (ab244212), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-LC3C antibody (ab244212)

    Cytospin-isolated human white blood cells labeling LC3C using ab244212 at a 1/200 dilutiom for 1 hr (red) in ICC/IF.

    Myeloperoxidase is green, Hoechst dye is blue.

  • Immunocytochemistry/ Immunofluorescence - Anti-LC3C antibody (ab244212), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-LC3C antibody (ab244212)

    Cytospin-isolated human white blood cells labeling LC3C using ab244212 at a 1/200 dilutiom for 1 hr (red) in ICC/IF.

    Myeloperoxidase is green, Hoechst dye is blue.

  • Western blot - Anti-LC3C antibody (ab244212), expandable thumbnail

    Western blot - Anti-LC3C antibody (ab244212)

    All lanes: Western blot - Anti-LC3C antibody (ab244212) at 1/100 dilution

    All lanes: HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

    Performed under reducing conditions.

    Predicted band size: 17 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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