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AB167421

Anti-LC3C antibody [EPR8076]

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(1 Publication)

Rabbit Recombinant Monoclonal LC3C antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Microtubule-associated proteins 1A/1B light chain 3C, Autophagy-related protein LC3 C, Autophagy-related ubiquitin-like modifier LC3 C, MAP1 light chain 3-like protein 3, MAP1A/MAP1B light chain 3 C, Microtubule-associated protein 1 light chain 3 gamma, MAP1A/MAP1B LC3 C, MAP1LC3C

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LC3C antibody [EPR8076] (AB167421)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LC3C antibody [EPR8076] (AB167421)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling LC3C with ab167421 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-LC3C antibody [EPR8076] (AB167421)
  • WB

Unknown

Western blot - Anti-LC3C antibody [EPR8076] (AB167421)

All lanes:

Western blot - Anti-LC3C antibody [EPR8076] (ab167421) at 1/10000 dilution

Lane 1:

LC3C transfected 293T cell lysate at 10 µg

Lane 2:

Mock transfected 293T cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP antibody at 1/2000 dilution

Predicted band size: 14 kDa,17 kDa,21 kDa,22 kDa,37 kDa,73 kDa

Observed band size: 24 kDa

false

  • Carrier free

    Anti-LC3C antibody [EPR8076] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8076

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein LC3C also known as microtubule-associated protein 1 light chain 3 gamma is part of the ATG8 family and is critical in the autophagy pathway. LC3C with a molecular mass of approximately 14.7 kDa participates in the formation of autophagosomes. This protein predominantly localizes in the cytoplasm and transitions to autophagosome membranes during autophagy. LC3C expression occurs in multiple tissues including the brain and liver indicating its broad role in cellular function.
Biological function summary

Microtubule-associated protein 1 light chain 3 gamma acts as an important component in the autophagy process. It is part of a complex that involves other homologs of the LC3 protein family. LC3C interacts directly with phosphatidylethanolamine on autophagosome membranes contributing significantly to the expansion and sealing of these structures. This action is critical for cellular homeostasis and survival during nutrient deprivation.

Pathways

The protein LC3C is an integral part of the macroautophagy pathway. It associates with proteins such as p62 (SQSTM1) which delivers ubiquitinated cargo to autophagosomes. LC3C also interacts with Beclin-1 a protein involved in the initiation of autophagy. These interactions place LC3C within the autophagic machinery essential for lysosomal degradation and recycling of cellular components facilitating proper cellular function.

Research indicates that microtubule-associated protein 1 light chain 3 gamma relates to cancer and neurodegenerative disorders. In cancer altered LC3C levels can affect tumor cell survival and adaptation to stress associating with proteins such as PINK1 which regulates mitochondrial autophagy. In neurodegenerative diseases improper autophagy due to dysfunctional LC3C may contribute to the accumulation of damaged proteins leading to conditions like Parkinson's disease where LC3C interacts with parkin an important player in targeting dysfunctional mitochondria for removal through mitophagy.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ubiquitin-like modifier that plays a crucial role in antibacterial autophagy (xenophagy) through the selective binding of CALCOCO2 (PubMed : 23022382). Recruits all ATG8 family members to infecting bacteria such as S.typhimurium (PubMed : 23022382). May also play a role in aggrephagy, the macroautophagic degradation of ubiquitinated and aggregated proteins (PubMed : 28404643).
See full target information MAP1LC3C
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 8:103626-103639 PubMed29262589

2017

ABR, a novel inducer of transcription factor C/EBPα, contributes to myeloid differentiation and is a favorable prognostic factor in acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Carolina Yaeko Namasu,Christiane Katzerke,Daniela Bräuer-Hartmann,Alexander Arthur Wurm,Dennis Gerloff,Jens-Uwe Hartmann,Sebastian Schwind,Carsten Müller-Tidow,Nadja Hilger,Stephan Fricke,Maximilian Christopeit,Dietger Niederwieser,Gerhard Behre
View all publications
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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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