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AB150367

Anti-LC3C antibody [EPR8077]

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(3 Publications)

Rabbit Recombinant Monoclonal LC3C antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Microtubule-associated protein 1 light chain 3 gamma, Autophagy-related protein LC3 C, Autophagy-related ubiquitin-like modifier LC3 C, MAP1 light chain 3-like protein 3, Microtubule-associated proteins 1A/1B light chain 3C, MAP1A/MAP1B LC3 C, MAP1A/MAP1B light chain 3 C, MAP1LC3C

1 Images
Western blot - Anti-LC3C antibody [EPR8077] (AB150367)
  • WB

Unknown

Western blot - Anti-LC3C antibody [EPR8077] (AB150367)

All lanes:

Western blot - Anti-LC3C antibody [EPR8077] (ab150367) at 1/10000 dilution

Lane 1:

LC3C transfected 293T cell lysates at 10 µg

Lane 2:

Mock-transfected 293T cell lysates at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 17 kDa

false

  • Carrier free

    Anti-LC3C antibody [EPR8077] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8077

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein LC3C also known as microtubule-associated protein 1 light chain 3 gamma is part of the ATG8 family and is critical in the autophagy pathway. LC3C with a molecular mass of approximately 14.7 kDa participates in the formation of autophagosomes. This protein predominantly localizes in the cytoplasm and transitions to autophagosome membranes during autophagy. LC3C expression occurs in multiple tissues including the brain and liver indicating its broad role in cellular function.
Biological function summary

Microtubule-associated protein 1 light chain 3 gamma acts as an important component in the autophagy process. It is part of a complex that involves other homologs of the LC3 protein family. LC3C interacts directly with phosphatidylethanolamine on autophagosome membranes contributing significantly to the expansion and sealing of these structures. This action is critical for cellular homeostasis and survival during nutrient deprivation.

Pathways

The protein LC3C is an integral part of the macroautophagy pathway. It associates with proteins such as p62 (SQSTM1) which delivers ubiquitinated cargo to autophagosomes. LC3C also interacts with Beclin-1 a protein involved in the initiation of autophagy. These interactions place LC3C within the autophagic machinery essential for lysosomal degradation and recycling of cellular components facilitating proper cellular function.

Research indicates that microtubule-associated protein 1 light chain 3 gamma relates to cancer and neurodegenerative disorders. In cancer altered LC3C levels can affect tumor cell survival and adaptation to stress associating with proteins such as PINK1 which regulates mitochondrial autophagy. In neurodegenerative diseases improper autophagy due to dysfunctional LC3C may contribute to the accumulation of damaged proteins leading to conditions like Parkinson's disease where LC3C interacts with parkin an important player in targeting dysfunctional mitochondria for removal through mitophagy.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ubiquitin-like modifier that plays a crucial role in antibacterial autophagy (xenophagy) through the selective binding of CALCOCO2 (PubMed : 23022382). Recruits all ATG8 family members to infecting bacteria such as S.typhimurium (PubMed : 23022382). May also play a role in aggrephagy, the macroautophagic degradation of ubiquitinated and aggregated proteins (PubMed : 28404643).
See full target information MAP1LC3C
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Autophagy 18:2985-3003 PubMed35414338

2022

LC3 subfamily in cardiolipin-mediated mitophagy: a comparison of the LC3A, LC3B and LC3C homologs.

Applications

Unspecified application

Species

Unspecified reactive species

Marina N Iriondo,Asier Etxaniz,Yaiza R Varela,Uxue Ballesteros,Javier H Hervás,L Ruth Montes,Félix M Goñi,Alicia Alonso

Cells 9: PubMed33081014

2020

Novel Insights into the Cellular Localization and Regulation of the Autophagosomal Proteins LC3A, LC3B and LC3C.

Applications

Unspecified application

Species

Unspecified reactive species

Marius W Baeken,Katja Weckmann,Philip Diefenthäler,Jan Schulte,Kamran Yusifli,Bernd Moosmann,Christian Behl,Parvana Hajieva

PloS one 8:e77782 PubMed24204963

2013

The immune system GTPase GIMAP6 interacts with the Atg8 homologue GABARAPL2 and is recruited to autophagosomes.

Applications

Unspecified application

Species

Unspecified reactive species

John C Pascall,Sergio Rotondo,Aamir S Mukadam,David Oxley,Judith Webster,Simon A Walker,Jerry Piron,Christine Carter,Nicholas T Ktistakis,Geoffrey W Butcher
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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