Rabbit Recombinant Monoclonal LCK antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Immunoprecipitation - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (AB229379), expandable thumbnail](https://content.abcam.com/products/images/lck-antibody-epr20798-107-bsa-and-azide-free-ab229379--immunoprecipitation-img149978.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (AB229379)")
![Western blot - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (AB229379), expandable thumbnail](https://content.abcam.com/products/images/lck-antibody-epr20798-107-bsa-and-azide-free-ab229379--western-blot-img168979.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (AB229379)")
![Flow Cytometry (Intracellular) - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (AB229379), expandable thumbnail](https://content.abcam.com/products/images/lck-antibody-epr20798-107-bsa-and-azide-free-ab229379--flow-cytometry-intracellular-img37353.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (AB229379)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (AB229379), expandable thumbnail](https://content.abcam.com/products/images/lck-antibody-epr20798-107-bsa-and-azide-free-ab229379--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125373.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (AB229379)")
![Flow Cytometry (Intracellular) - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (AB229379), expandable thumbnail](https://content.abcam.com/products/images/lck-antibody-epr20798-107-bsa-and-azide-free-ab229379--flow-cytometry-intracellular-img32613.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (AB229379)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Expected | Tested | Tested | Tested | Tested |
| Rat | Expected | Tested | Expected | Expected | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
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Target data
Function
Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosine residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP. Interacts with FYB2 (PubMed:27335501).
Alternative names
Tyrosine-protein kinase Lck, Leukocyte C-terminal Src kinase, Lymphocyte cell-specific protein-tyrosine kinase, Protein YT16, Proto-oncogene Lck, T cell-specific protein-tyrosine kinase, p56-LCK, LSK, LCK
Recommended products
Rabbit Recombinant Monoclonal LCK antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20798-107
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab229379 is the carrier-free version of Anti-Lck antibody [EPR20798-107] ab227975.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Lck also known as lymphocyte-specific protein tyrosine kinase plays an important role in T-cell activation. It serves as an enzyme and is integral in the signal transduction of the immune system. Lck has a molecular weight of approximately 56 kDa. It is expressed predominantly in T lymphocytes and is part of the Src family kinases. This protein is important in phosphorylating substrates leading to downstream signaling events that are necessary for the proper immune function.
- Biological function summary
Lck initiates and propagates T-cell receptor (TCR) signaling cascades. It interacts with the CD4 and CD8 co-receptors and triggers the activation of other kinases within the cell. Lck as part of a signaling complex associates with proteins such as ZAP-70 and LAT facilitating the assembly of larger molecular machines necessary for immune responses. Its activity is tightly regulated by phosphorylation with key sites being tyrosine 394 and 505.
- Pathways
The Lck protein plays an essential role in TCR signaling and immune responses. It actively participates in the Lck signaling pathway setting off cascades that lead to T-cell activation and differentiation. It interacts closely with other proteins like Fyn and Src making sure that the pathway progresses correctly. In conjunction with the immune synapse Lck ensures the transmission of signals that allow the T-cells to respond to external antigens efficiently.
- Associated diseases and disorders
Defective Lck activity can contribute to immunodeficiencies and autoimmune diseases. Proper Lck function is linked to conditions like Severe Combined Immunodeficiency (SCID) and lupus. Dysregulation in Lck activity may also affect proteins such as ZAP-70 worsening disease progression. Understanding Lck's participation in these conditions can lead to better therapeutic interventions and aid in developing targeted treatments for immune-related disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
![Immunoprecipitation - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379), expandable thumbnail](https://content.abcam.com/products/images/lck-antibody-epr20798-107-bsa-and-azide-free-ab229379--immunoprecipitation-img149978.jpg "Immunoprecipitation - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379)")
Immunoprecipitation - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379)
Lck was immunoprecipitated from 0.35 mg of Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with Anti-Lck antibody [EPR20798-107] ab227975 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-Lck antibody [EPR20798-107] ab227975 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: Jurkat whole cell lysate 10 μg (Input).
Lane 2: Anti-Lck antibody [EPR20798-107] ab227975 IP in Jurkat whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Lck antibody [EPR20798-107] ab227975 in Jurkat whole cell lysate.Exposure time: 8 seconds.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lck antibody [EPR20798-107] ab227975).
All lanes: Immunoprecipitation - Anti-Lck antibody [EPR20798-107] (Anti-Lck antibody [EPR20798-107] ab227975)
Developed using the ECL technique.
Predicted band size: 58 kDa
Observed band size: 58 kDa
![Western blot - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379), expandable thumbnail](https://content.abcam.com/products/images/lck-antibody-epr20798-107-bsa-and-azide-free-ab229379--western-blot-img168979.jpg "Western blot - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379)")
Western blot - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379)
False colour image of Western blot: Anti-Lck antibody [EPR20798-107] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-Lck antibody [EPR20798-107] ab227975 was shown to bind specifically to Lck. A band was observed at 60 kDa in wild-type Jurkat cell lysates with no signal observed at this size in Lck knockout cell line Human LCK knockout Jurkat cell line ab273855 (knockout cell lysate Human LCK knockout Jurkat cell lysate ab273809). To generate this image, wild-type and Lck knockout Jurkat cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lck antibody [EPR20798-107] ab227975).
All lanes: Western blot - Anti-Lck antibody [EPR20798-107] (Anti-Lck antibody [EPR20798-107] ab227975) at 1/1000 dilution
Lane 1: Wild-type Jurkat cell lysate at 20 µg
Lane 2: Lck knockout Jurkat cell lysate at 20 µg
Lane 2: Western blot - Human LCK knockout Jurkat cell line (Human LCK knockout Jurkat cell line ab273855)
Lane 3: Ramos cell lysate at 20 µg
Lane 4: A549 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 60 kDa
![Flow Cytometry (Intracellular) - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379), expandable thumbnail](https://content.abcam.com/products/images/lck-antibody-epr20798-107-bsa-and-azide-free-ab229379--flow-cytometry-intracellular-img37353.jpg "Flow Cytometry (Intracellular) - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379)")
Flow Cytometry (Intracellular) - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized �WEHI-231 (mouse lymphoblast B cell lymphoma cell line) cell line labeling �Lck with Anti-Lck antibody [EPR20798-107] ab227975 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor� 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lck antibody [EPR20798-107] ab227975).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379), expandable thumbnail](https://content.abcam.com/products/images/lck-antibody-epr20798-107-bsa-and-azide-free-ab229379--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125373.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379)
Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Lck with Anti-Lck antibody [EPR20798-107] ab227975 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Membranous and cytoplasmic staining in T cells of rat colon is observed (PMID: 16769579). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lck antibody [EPR20798-107] ab227975).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379), expandable thumbnail](https://content.abcam.com/products/images/lck-antibody-epr20798-107-bsa-and-azide-free-ab229379--flow-cytometry-intracellular-img32613.jpg "Flow Cytometry (Intracellular) - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379)")
Flow Cytometry (Intracellular) - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized �Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling �Lck with Anti-Lck antibody [EPR20798-107] ab227975 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor� 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lck antibody [EPR20798-107] ab227975).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379), expandable thumbnail](https://content.abcam.com/products/images/lck-antibody-epr20798-107-bsa-and-azide-free-ab229379--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107929.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Lck with Anti-Lck antibody [EPR20798-107] ab227975 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Membranous and cytoplasmic staining in mouse spleen reactive lymph node and T cells is observed (PMID: 16769579). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lck antibody [EPR20798-107] ab227975).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379), expandable thumbnail](https://content.abcam.com/products/images/lck-antibody-epr20798-107-bsa-and-azide-free-ab229379--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img80798.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379)
Immunohistochemical analysis of paraffin-embedded human diffuse large B-cell lymphoma tissue labeling Lck with Anti-Lck antibody [EPR20798-107] ab227975 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Membranous and cytoplasmic staining in human diffuse large B-cell lymphoma is observed (PMID:16769579). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lck antibody [EPR20798-107] ab227975).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379), expandable thumbnail](https://content.abcam.com/products/images/lck-antibody-epr20798-107-bsa-and-azide-free-ab229379--immunocytochemistry-immunofluorescence-img44865.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379)")
Immunocytochemistry/ Immunofluorescence - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized WEHI-231 (mouse lymphoblast B cell lymphoma cell line) cells labeling Lck with Anti-Lck antibody [EPR20798-107] ab227975 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on WEHI-231 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lck antibody [EPR20798-107] ab227975).
![Immunocytochemistry/ Immunofluorescence - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379), expandable thumbnail](https://content.abcam.com/products/images/lck-antibody-epr20798-107-bsa-and-azide-free-ab229379--immunocytochemistry-immunofluorescence-img98978.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379)")
Immunocytochemistry/ Immunofluorescence - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Ramos (human Burkitt's lymphoma cell line) cells labeling Lck with Anti-Lck antibody [EPR20798-107] ab227975 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on Ramos cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lck antibody [EPR20798-107] ab227975).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379), expandable thumbnail](https://content.abcam.com/products/images/lck-antibody-epr20798-107-bsa-and-azide-free-ab229379--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107929.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379), expandable thumbnail](https://content.abcam.com/products/images/lck-antibody-epr20798-107-bsa-and-azide-free-ab229379--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img80798.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379)")
![Immunocytochemistry/ Immunofluorescence - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379), expandable thumbnail](https://content.abcam.com/products/images/lck-antibody-epr20798-107-bsa-and-azide-free-ab229379--immunocytochemistry-immunofluorescence-img44865.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379)")
![Immunocytochemistry/ Immunofluorescence - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379), expandable thumbnail](https://content.abcam.com/products/images/lck-antibody-epr20798-107-bsa-and-azide-free-ab229379--immunocytochemistry-immunofluorescence-img98978.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Lck antibody [EPR20798-107] - BSA and Azide free (ab229379)")
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