Rabbit Polyclonal LCLAT1 antibody. Suitable for IHC-P, ICC and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human LCLAT1 aa 200-350.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human LCLAT1 aa 200-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q6UWP7
Reactivity data
Select an application| IHC-P | ICC | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Fixation/Permeabilization: PFA/Triton X-100 |
Associated Products
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Target data
Function
Exhibits acyl-CoA:lysocardiolipin acyltransferase (ALCAT) activity; catalyzes the reacylation of lyso-cardiolipin to cardiolipin (CL), a key step in CL remodeling (By similarity). Recognizes both monolysocardiolipin and dilysocardiolipin as substrates with a preference for linoleoyl-CoA and oleoyl-CoA as acyl donors (By similarity). Also exhibits 1-acyl-sn-glycerol-3-phosphate acyltransferase activity (AGPAT) activity; converts 1-acyl-sn-glycerol-3- phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3- phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (PubMed:16620771). Possesses both lysophosphatidylinositol acyltransferase (LPIAT) and lysophosphatidylglycerol acyltransferase (LPGAT) activities (PubMed:19075029). Required for establishment of the hematopoietic and endothelial lineages (By similarity).
Alternative names
AGPAT8, ALCAT1, LYCAT, UNQ1849/PRO3579, LCLAT1, Lysocardiolipin acyltransferase 1, 1-acylglycerol-3-phosphate O-acyltransferase 8, Acyl-CoA:lysocardiolipin acyltransferase 1, 1-AGP acyltransferase 8, 1-AGPAT 8
Recommended products
Rabbit Polyclonal LCLAT1 antibody. Suitable for IHC-P, ICC and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human LCLAT1 aa 200-350.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human LCLAT1 aa 200-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q6UWP7
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Lysocardiolipin acyltransferase 1 (LCLAT1) also known as monolysocardiolipin acyltransferase (MLCL AT-1) is an enzyme with a mass of approximately 58 kDa. LCLAT1 resides primarily in the mitochondria and plays a significant role in phospholipid metabolism by catalyzing the reacylation of monolysocardiolipin (MLCL) to cardiolipin (CL). This function is critical for maintaining mitochondrial membrane structure and function. Expression of LCLAT1 occurs in various tissues with notable presence in heart and skeletal muscle where high energy demand correlates with mitochondrial activity.
- Biological function summary
LCLAT1 functions within the lipid remodeling pathway specific to the mitochondria and is an important player in the maintenance of mitochondrial function and energy metabolism. This enzyme acts independently not as part of a larger protein complex. LCLAT1 ensures the proper composition of cardiolipin which is essential for the optimal performance of respiratory chain complexes and cellular energy homeostasis.
- Pathways
The enzyme LCLAT1 plays a vital role in lipid metabolism particularly the remodeling pathway of cardiolipin in mitochondria. This pathway is vital for the functionality of the electron transport chain an important element in cellular respiration. LCLAT1 interconnects with Tafazzin (TAZ) another enzyme critical in cardiolipin biosynthesis and remodeling highlighting its participation in maintaining mitochondrial health and efficient aerobic energy supply.
- Associated diseases and disorders
Alterations in LCLAT1 function have connections to mitochondrial diseases due to its role in cardiolipin remodeling. Disruptions in its activity may relate to Barth syndrome a genetic disorder linked to defects in cardiolipin metabolism. LCLAT1's association with Tafazzin (TAZ) further implicates it in the disorder as both proteins are essential for proper cardiolipin function and mitochondrial integrity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Immunocytochemistry - Anti-LCLAT1 antibody (ab122197)
Immunocytochemistry/immunofluorescence analysis of human cell line A-431 shows labelling LCLAT1 with ab122197 at 2 μg/mL.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LCLAT1 antibody (ab122197)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart muscle tissue labelling LCLAT1 with ab122197 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LCLAT1 antibody (ab122197)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human duodenum tissue labelling LCLAT1 with ab122197 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LCLAT1 antibody (ab122197)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling LCLAT1 with ab122197 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LCLAT1 antibody (ab122197)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fallopian tube tissue labelling LCLAT1 with ab122197 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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