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AB238593

Anti-LCT antibody

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(1 Publication)

Anti-LCT antibody (ab238593) is a rabbit polyclonal antibody detecting LCT in IHC-P. Suitable for Human.

View Alternative Names

LPH, LCT, Lactase/phlorizin hydrolase, Lactase/glycosylceramidase

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LCT antibody (AB238593)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LCT antibody (AB238593)

Immunohistochemistry analysis of paraffin-embedded sections of human colorectal cancer staining with ab238593 at 1/100 dilution and secondary Goat anti-rabbit polymer IgG HRP. 0.05% DAB. Secondary antibody only control : uses 1% BSA instead of primary antibody

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human LCT aa 950-1300. The exact immunogen used to generate this antibody is proprietary information.

P09848

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-LCT antibody (ab238593) is a rabbit polyclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LCT also known as lactase-phlorizin hydrolase is an enzyme that mechanically breaks down lactose into glucose and galactose. This enzyme weighs approximately 160 kDa. It primarily localizes in the small intestine where it resides on the brush border of the enterocytes. The high expression levels in infants allow them to digest the lactose in breast milk effectively but expression can decrease with age depending on genetic factors.
Biological function summary

LCT functions to enable the proper digestion and absorption of lactose critical for energy production in lactose-consuming individuals. It belongs to the glycoside hydrolase family and operates effectively at the intestinal surface. LCT does not work alone but collaborates with other small intestine enzymes that further aid in carbohydrate digestion.

Pathways

The digestion process involving LCT plays an important role in lactose metabolism and the subsequent galactose metabolism pathway. It interfaces intimately with proteins such as SGLT1 (sodium-dependent glucose transporter 1) which facilitate the uptake of glucose and galactose across the intestinal membrane. This synergy allows for the efficient conversion of ingested lactose into absorbable sugars which enter glycolytic pathways.

LCT relates closely to lactose intolerance and congenital lactase deficiency. In lactose intolerance insufficient LCT activity results in undigested lactose in the colon causing symptoms like bloating and diarrhea. Mutations affecting the LCT gene or its regulatory components can lead to congenital lactase deficiency a more severe hereditary form present from birth. Reduced expression or functionality of LCT interacts with increased activity of bacterial beta-galactosidase in the gut exacerbating symptoms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Broad specificity glycosidase of the intestinal brush border membrane that hydrolyzes lactose, the main sugar in mammalian milk, to produce D-glucose and D-galactose (PubMed : 12594539, PubMed : 16400612, PubMed : 3929764, PubMed : 9762914). The mature protein is composed of two domains that catalyze the hydrolysis of beta-glucopyranosides and beta-galactopyranosides, with a preference for hydrophilic aglycones (in lactose and cellobiose) for one domain and hydrophobic aglycones (in phlorizin and glycosylceramides) for the other (PubMed : 12594539, PubMed : 3929764, PubMed : 9762914).
See full target information LCT
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science advances 7:eabf6063 PubMed34705506

2021

An EMT-primary cilium-GLIS2 signaling axis regulates mammogenesis and claudin-low breast tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Molly M Wilson,Céline Callens,Matthieu Le Gallo,Svetlana Mironov,Qiong Ding,Amandine Salamagnon,Tony E Chavarria,Roselyne Viel,Abena D Peasah,Arjun Bhutkar,Sophie Martin,Florence Godey,Patrick Tas,Hong Soon Kang,Philippe P Juin,Anton M Jetten,Jane E Visvader,Robert A Weinberg,Massimo Attanasio,Claude Prigent,Jacqueline A Lees,Vincent J Guen
View all publications
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