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AB235340

Anti-LDAH antibody

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(1 Publication)

Rabbit Polyclonal LDAH antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human LDAH.

View Alternative Names

C2orf43, LDAH, Lipid droplet-associated hydrolase, Lipid droplet-associated serine hydrolase, hLDAH

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDAH antibody (AB235340)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDAH antibody (AB235340)

Paraffin-embedded human testis tissue stained for LDAH with ab235340 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDAH antibody (AB235340)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDAH antibody (AB235340)

Paraffin-embedded human pancreatic tissue stained for LDAH with ab235340 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDAH antibody (AB235340)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDAH antibody (AB235340)

Paraffin-embedded human adrenal gland tissue stained for LDAH with ab235340 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-LDAH antibody (AB235340)
  • WB

Supplier Data

Western blot - Anti-LDAH antibody (AB235340)

All lanes:

Western blot - Anti-LDAH antibody (ab235340) at 1/500 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 3:

A431 (Human epidermoid carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to Rabbit IgG at 1/10000 dilution

Predicted band size: 37 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human LDAH.

Q9H6V9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LDAH also known as Lipid Dehydrogenase functions as an enzyme involved in lipid metabolism. Its mass is approximately 45 kDa. This protein is highly expressed in tissues with active lipid metabolism such as the liver and adipose tissue. LDAH plays a role in lipid oxidation aiding in the conversion of lipid substrates into various metabolic intermediates. The enzyme ensures efficient lipid utilization contributing to energy homeostasis within the cell.
Biological function summary

The enzyme LDAH facilitates the breakdown and conversion of lipid molecules optimizing cellular energy balance. LDAH operates as an important component within a larger enzymatic complex that includes other dehydrogenases. The interactions and activities within this complex regulate the lipid metabolic processes influencing the energy output available for cellular functions. LDAH's activity is modulated through its interaction with co-factors and substrates impacting its efficiency and metabolic outcomes.

Pathways

LDAH integrates into essential lipid metabolism pathways such as the fatty acid beta-oxidation pathway. This pathway is critical for breaking down fatty acids into acetyl-CoA a molecule pivotal in energy supply through the citric acid cycle. LDAH's function complements the roles of related proteins like Acyl-CoA dehydrogenase aiding in the removal of electrons from fatty acids during metabolic processes. These relationships position LDAH as an important player in maintaining cellular energy dynamics and responding to metabolic demands.

Altered LDAH activity connects to metabolic syndrome and non-alcoholic fatty liver disease (NAFLD). Dysregulation of LDAH can lead to impaired lipid metabolism resulting in fat accumulation and insulin resistance key characteristics of these conditions. LDAH's interaction with proteins such as Acyl-CoA dehydrogenase and regulators of mitochondrial function highlights its role in these diseases. Researchers study LDAH as a potential therapeutic target for managing lipid-related metabolic disorders seeking ways to modulate its function to restore metabolic balance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable serine lipid hydrolase associated with lipid droplets (By similarity). Has low cholesterol esterase activity (By similarity). Appears to lack triglyceride lipase activity (By similarity). Involved in cholesterol and triglyceride homeostasis; has opposing effects, stimulating cellular triglyceride accumulation and cellular cholesterol release (PubMed : 24357060, PubMed : 28578400). Acts antagonistically with PNPLA2/ATGL in regulation of cellular lipid stores (PubMed : 28578400). May regulate triglyceride accumulation indirectly through stimulation of PNPLA2/ATGL ubiquitination and proteasomal degradation (PubMed : 28578400). Promotes microtubule-dependent lipid droplet fusion (PubMed : 28578400). Highly expressed in macrophage-rich areas in atherosclerotic lesions, suggesting that it could promote cholesterol ester turnover in macrophages (By similarity).
See full target information LDAH
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 40:e106336 PubMed33595114

2021

NHP2 downregulation counteracts hTR-mediated activation of the DNA damage response at ALT telomeres.

Applications

Unspecified application

Species

Unspecified reactive species

Maya Raghunandan,Dan Geelen,Eva Majerova,Anabelle Decottignies
View all publications
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