Anti-LDB1 antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LDB1 antibody (AB96799)

HeLa cells stained for LDB1 (green) using ab96799 at 1/500 dilution in ICC/IF.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDB1 antibody (AB96799)

Immunohistochemical analysis of paraffin-embedded U87 xenograft using ab96799 at a dilution of 1/100.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LDB1 antibody (AB96799)

Immunofluorescence analysis of paraformaldheyde-fixed HeLa cells using ab96799 at a dilution of 1/200.

• IP

Supplier Data

Immunoprecipitation - Anti-LDB1 antibody (AB96799)

LDB1 was immunoprecipitated from 1000 μg 293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab96799. Western blot was performed from the immunoprecipitate using 2.5 μg of ab96799 at 1/1000 dilution.

Lane A : 20 μg 293T whole cell lysate/extract.
Lane B : Control with 2.5 μg of preimmune rabbit IgG.
Lane C : ab96799 IP in 293T (12% SDS-PAGE).

All lanes:

Immunoprecipitation - Anti-LDB1 antibody (ab96799)

Predicted band size: 47 kDa

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• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDB1 antibody (AB96799)

Paraffin-embedded rat middle brain tissue stained for LDB1 using ab96799 at a/500 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDB1 antibody (AB96799)

Paraffin-embedded mouse muscle tissue stained for LDB1 using ab96799 at 1/500 dilution in immunohistochemical analysis.

• WB

Supplier Data

Western blot - Anti-LDB1 antibody (AB96799)

All lanes:

Western blot - Anti-LDB1 antibody (ab96799) at 1/1000 dilution

All lanes:

Mouse brain lysate at 50 µg

Predicted band size: 47 kDa

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• WB

Supplier Data

Western blot - Anti-LDB1 antibody (AB96799)

All lanes:

Western blot - Anti-LDB1 antibody (ab96799) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 47 kDa

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• WB

Unknown

Western blot - Anti-LDB1 antibody (AB96799)

All lanes:

Western blot - Anti-LDB1 antibody (ab96799) at 1/1000 dilution

All lanes:

Raji whole cell lysate at 30 µg

Predicted band size: 47 kDa

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• WB

CiteAb

Western blot - Anti-LDB1 antibody (AB96799)

LDB1 western blot using anti-LDB1 antibody ab96799. Publication image and figure legend from Stanulovic, V. S., Cauchy, P., et al., 2017, Nucleic Acids Res, PubMed 28973433.

ab96799 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab96799 please see the product overview.

Gene expression analysis of Tal1-/- Flk-1+ cells. (A) Lmo2, Tal1 gene expression relative to Hprt in Flk-1+ cells derived from WT, Lmo2-/- or Tal1-/- ES cells. Data represent the mean of three independent samples measured in duplicate ± standard deviation. (B) LMO2, TAL1, LDB1 and β-actin protein levels detected by western blot using nuclear extract from WT, Lmo2-/- and Tal1-/- Flk-1+ cells. (C) Pairwise comparison of significantly differentially expressed genes. Quantitation and analyses of RNAseq data are based on biological triplicate samples. (D) Heat map showing hierarchical clustering of differentially expressed genes at the Flk-1+ stage. (E) Gene ontology enrichment analysis for biological process was performed on cluster 5 as identified in D. Terms were ordered according to their Modified Fisher Extract P-value and only terms with p < 0.05 were considered significant.

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