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AB3627

Anti-LDB2 antibody

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(1 Review)

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(2 Publications)

Rabbit Polyclonal LDB2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human LDB2 aa 100-150 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CLIM1, LDB2, LIM domain-binding protein 2, LDB-2, Carboxyl-terminal LIM domain-binding protein 1, LIM domain-binding factor CLIM1, CLIM-1

1 Images
Western blot - Anti-LDB2 antibody (AB3627)
  • WB

Unknown

Western blot - Anti-LDB2 antibody (AB3627)

Western blot using ab3627 at 1/500.

Lane 1 : Human Kidney
Lane 2 : Mouse Spleen

Lysates at 18μg per lane.
Secondary ab : Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed
ab7090 (1/5000).
Exposure time : 1 minute.

All lanes:

Western blot - Anti-LDB2 antibody (ab3627)

Predicted band size: 43 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human LDB2 aa 100-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O43679

Specificity

This affinity purified antibody is directed against mouse LDB2. A BLAST analysis was used to suggest reactivity with this protein from mouse, human, chimpanzee, dog, rat and chicken based on 100% homology for the immunogen sequence. Cross reactivity with LDB2 homologues from other sources has not been determined.

This antibody detects a single clean band representing LDB2 at 42kD in human kidney and mouse spleen.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This affinity purified antibody is directed against mouse LDB2. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LIM Domain Binding 2 (LDB2) also known as CLIM2 or NLI binds to LIM domain-containing proteins. This protein with a molecular weight of approximately 48 kDa plays a significant role in transcriptional regulation. LDB2 is mainly expressed in neural and hematopoietic tissues where it influences the development and differentiation processes. Through its ability to interact with a variety of LIM-homeodomain proteins LDB2 modulates complex cellular functions.
Biological function summary

LIM Domain Binding 2 acts as a scaffold protein facilitating the assembly of transcriptional complexes. It actively participates in the gene regulation by forming multi-protein complexes with other transcription factors. LDB2 helps stabilize these complexes enabling the precise control of gene expression. Its role in the broader transcriptional machinery allows it to influence cell fate decisions and differentiation pathways significantly.

Pathways

LIM Domain Binding 2 contributes to important signaling cascades such as the Wnt and TGF-beta pathways. In these pathways LDB2 works alongside proteins like beta-catenin and SMADs contributing to cell proliferation differentiation and apoptosis processes. By interacting with these diverse pathways LDB2 integrates signals from different sources and helps orchestrate cellular responses that are important for normal development and homeostasis.

LIM Domain Binding 2 has links to certain cancers and developmental disorders. Its misregulation associates with cancers often involving aberrant activation of beta-catenin-driven Wnt signaling. Furthermore disruptions in LDB2 interactions with other cytoskeletal proteins can lead to neural developmental disorders. Understanding LDB2's role in these contexts offers potential therapeutic insights and highlights its importance in maintaining cellular balance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription cofactor. Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors.
See full target information LDB2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 4:1039 PubMed34489520

2021

Generation and characterization of cardiac valve endothelial-like cells from human pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

LinXi Cheng,MingHui Xie,WeiHua Qiao,Yu Song,YanYong Zhang,YingChao Geng,WeiLin Xu,Lin Wang,Zheng Wang,Kai Huang,NianGuo Dong,YuHua Sun

Molecular biology of the cell 20:4174-82 PubMed19675209

2009

The Ldb1 and Ldb2 transcriptional cofactors interact with the Ste20-like kinase SLK and regulate cell migration.

Applications

ICC/IF

Species

Mouse

Chris J Storbeck,Simona Wagner,Paul O'Reilly,Marlene McKay,Robin J Parks,Heiner Westphal,Luc A Sabourin
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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