Rabbit Polyclonal LDB2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human LDB2 aa 100-150 conjugated to Keyhole Limpet Haemocyanin.
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Publications
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- Communications biology 4:10392021Generation and characterization of cardiac valve endothelial-like cells from human pluripotent stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinXi Cheng,MingHui Xie,WeiHua Qiao,Yu Song,YanYong Zhang,YingChao Geng,WeiLin Xu,Lin Wang,Zheng Wang,Kai Huang,NianGuo Dong,YuHua SunPubMed 34489520
- Molecular biology of the cell 20:4174-822009The Ldb1 and Ldb2 transcriptional cofactors interact with the Ste20-like kinase SLK and regulate cell migration.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human LDB2 aa 100-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link O43679
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Tested | Expected |
| Chicken | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/2000 | Notes - |
Species Human | Dilution info 1/500 - 1/2000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
Species Mouse | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken | Dilution info - | Notes - |
Target data
Function
Transcription cofactor. Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors.
Alternative names
CLIM1, LDB2, LIM domain-binding protein 2, LDB-2, Carboxyl-terminal LIM domain-binding protein 1, LIM domain-binding factor CLIM1, CLIM-1
Recommended products
Rabbit Polyclonal LDB2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human LDB2 aa 100-150 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human LDB2 aa 100-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link O43679
- Purification technique
- Affinity purification Immunogen
- Specificity
This affinity purified antibody is directed against mouse LDB2. A BLAST analysis was used to suggest reactivity with this protein from mouse, human, chimpanzee, dog, rat and chicken based on 100% homology for the immunogen sequence. Cross reactivity with LDB2 homologues from other sources has not been determined.
This antibody detects a single clean band representing LDB2 at 42kD in human kidney and mouse spleen.
- Concentration
- Purification notes
This affinity purified antibody is directed against mouse LDB2. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LIM Domain Binding 2 (LDB2) also known as CLIM2 or NLI binds to LIM domain-containing proteins. This protein with a molecular weight of approximately 48 kDa plays a significant role in transcriptional regulation. LDB2 is mainly expressed in neural and hematopoietic tissues where it influences the development and differentiation processes. Through its ability to interact with a variety of LIM-homeodomain proteins LDB2 modulates complex cellular functions.
- Biological function summary
LIM Domain Binding 2 acts as a scaffold protein facilitating the assembly of transcriptional complexes. It actively participates in the gene regulation by forming multi-protein complexes with other transcription factors. LDB2 helps stabilize these complexes enabling the precise control of gene expression. Its role in the broader transcriptional machinery allows it to influence cell fate decisions and differentiation pathways significantly.
- Pathways
LIM Domain Binding 2 contributes to important signaling cascades such as the Wnt and TGF-beta pathways. In these pathways LDB2 works alongside proteins like beta-catenin and SMADs contributing to cell proliferation differentiation and apoptosis processes. By interacting with these diverse pathways LDB2 integrates signals from different sources and helps orchestrate cellular responses that are important for normal development and homeostasis.
- Associated diseases and disorders
LIM Domain Binding 2 has links to certain cancers and developmental disorders. Its misregulation associates with cancers often involving aberrant activation of beta-catenin-driven Wnt signaling. Furthermore disruptions in LDB2 interactions with other cytoskeletal proteins can lead to neural developmental disorders. Understanding LDB2's role in these contexts offers potential therapeutic insights and highlights its importance in maintaining cellular balance.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-LDB2 antibody (ab3627)
Western blot using ab3627 at 1/500.
Lane 1: Human Kidney
Lane 2: Mouse SpleenLysates at 18μg per lane.
Secondary ab: Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab7090 (1/5000).
Exposure time: 1 minute.All lanes: Western blot - Anti-LDB2 antibody (ab3627)
Predicted band size: 43 kDa
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