Goat Polyclonal LDB3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human LDB3 aa 250-300.
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Publications
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- Molecular therapy. Nucleic acids 34:1020602023Efficacy of exon-skipping therapy for DMD cardiomyopathy with mutations in actin binding domain 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNaoko Shiba,Xiao Yang,Mitsuto Sato,Shin Kadota,Yota Suzuki,Masahiro Agata,Kohei Nagamine,Masaki Izumi,Yusuke Honda,Tomoya Koganehira,Hideki Kobayashi,Hajime Ichimura,Shinichiro Chuma,Junichi Nakai,Shugo Tohyama,Keiichi Fukuda,Daigo Miyazaki,Akinori Nakamura,Yuji ShibaPubMed 38028197
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human LDB3 aa 250-300. The exact immunogen used to generate this antibody is proprietary information. Database link O75112
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Dog | Predicted | Predicted |
| Pig | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.01000-0.03000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, Pig | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 3.75 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
May function as an adapter in striated muscle to couple protein kinase C-mediated signaling via its LIM domains to the cytoskeleton.
Alternative names
KIAA0613, ZASP, LDB3, LIM domain-binding protein 3, Protein cypher, Z-band alternatively spliced PDZ-motif protein
Recommended products
Goat Polyclonal LDB3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human LDB3 aa 250-300.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human LDB3 aa 250-300. The exact immunogen used to generate this antibody is proprietary information. Database link O75112
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LDB3 also known as LIM domain-binding protein 3 or ZASP plays a mechanical role in muscle function. It is a protein of approximately 80-110 kDa depending on its various isoforms. LDB3 is mainly expressed in cardiac and skeletal muscle tissues where it localizes at the Z-lines within the sarcomeres. It interacts with other structural proteins to provide stability impacting muscle contraction and integrity.
- Biological function summary
In the context of muscle function LDB3 is an essential component of the sarcomere where it participates in a protein complex that includes alpha-actinin. This association helps maintain the structural organization of muscles and supports the force transmission required for muscle contractility. Furthermore LDB3 regulates protein-protein interactions contributing to muscle architecture and signaling pathways.
- Pathways
The LDB3 protein operates in the context of muscle cell signaling and structural pathways. LDB3 connects to the integrin-linked kinase (ILK) pathway which influences cell survival and muscle differentiation. It also plays a role in the actin cytoskeleton pathway where it associates with proteins such as filamin and myotilin supporting muscle cell stability and dynamics.
- Associated diseases and disorders
LDB3 has significant connections to cardiomyopathy and myofibrillar myopathy. Mutations or deficiencies in LDB3 can lead to these conditions affecting muscle function and leading to symptoms such as muscle weakness or cardiac complications. LDB3 disorders can involve interactions with proteins like desmin which when mutated exacerbate muscle tissue disorganization and disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDB3 antibody (ab110003)
ab110003, at 3.75 µg/ml, staining LDB3 in Human skeletal muscle by immunohistochemistry.
Western blot - Anti-LDB3 antibody (ab110003)
All lanes: Western blot - Anti-LDB3 antibody (ab110003) at 0.01 µg/mL
All lanes: Human muscle lysate in RIPA buffer at 35 µg
Predicted band size: 77 kDa
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