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AB110003

Anti-LDB3 antibody

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(3 Publications)

Goat Polyclonal LDB3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human LDB3 aa 250-300.

View Alternative Names

KIAA0613, ZASP, LDB3, LIM domain-binding protein 3, Protein cypher, Z-band alternatively spliced PDZ-motif protein

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDB3 antibody (AB110003)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDB3 antibody (AB110003)

ab110003, at 3.75 µg/ml, staining LDB3 in Human skeletal muscle by immunohistochemistry.

Western blot - Anti-LDB3 antibody (AB110003)
  • WB

Unknown

Western blot - Anti-LDB3 antibody (AB110003)

All lanes:

Western blot - Anti-LDB3 antibody (ab110003) at 0.01 µg/mL

All lanes:

Human muscle lysate in RIPA buffer at 35 µg

Predicted band size: 77 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human LDB3 aa 250-300. The exact immunogen used to generate this antibody is proprietary information.

O75112

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Alternative Products

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

AB47003

Anti-Cardiac Troponin I antibody

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01-0.03 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3.75 µg/mL", "IHCP-species-notes": "<p></p>" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LDB3 also known as LIM domain-binding protein 3 or ZASP plays a mechanical role in muscle function. It is a protein of approximately 80-110 kDa depending on its various isoforms. LDB3 is mainly expressed in cardiac and skeletal muscle tissues where it localizes at the Z-lines within the sarcomeres. It interacts with other structural proteins to provide stability impacting muscle contraction and integrity.
Biological function summary

In the context of muscle function LDB3 is an essential component of the sarcomere where it participates in a protein complex that includes alpha-actinin. This association helps maintain the structural organization of muscles and supports the force transmission required for muscle contractility. Furthermore LDB3 regulates protein-protein interactions contributing to muscle architecture and signaling pathways.

Pathways

The LDB3 protein operates in the context of muscle cell signaling and structural pathways. LDB3 connects to the integrin-linked kinase (ILK) pathway which influences cell survival and muscle differentiation. It also plays a role in the actin cytoskeleton pathway where it associates with proteins such as filamin and myotilin supporting muscle cell stability and dynamics.

LDB3 has significant connections to cardiomyopathy and myofibrillar myopathy. Mutations or deficiencies in LDB3 can lead to these conditions affecting muscle function and leading to symptoms such as muscle weakness or cardiac complications. LDB3 disorders can involve interactions with proteins like desmin which when mutated exacerbate muscle tissue disorganization and disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May function as an adapter in striated muscle to couple protein kinase C-mediated signaling via its LIM domains to the cytoskeleton.
See full target information LDB3
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38928252

2024

Association between Pro26Ser and Inclusion Body Myopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Daniela Piga,Simona Zanotti,Michela Ripolone,Laura Napoli,Patrizia Ciscato,Sara Gibertini,Lorenzo Maggi,Francesco Fortunato,Andrea Rigamonti,Dario Ronchi,Giacomo Pietro Comi,Stefania Corti,Monica Sciacco

Molecular therapy. Nucleic acids 34:102060 PubMed38028197

2023

Efficacy of exon-skipping therapy for DMD cardiomyopathy with mutations in actin binding domain 1.

Applications

Unspecified application

Species

Unspecified reactive species

Naoko Shiba,Xiao Yang,Mitsuto Sato,Shin Kadota,Yota Suzuki,Masahiro Agata,Kohei Nagamine,Masaki Izumi,Yusuke Honda,Tomoya Koganehira,Hideki Kobayashi,Hajime Ichimura,Shinichiro Chuma,Junichi Nakai,Shugo Tohyama,Keiichi Fukuda,Daigo Miyazaki,Akinori Nakamura,Yuji Shiba

Cell death & disease 9:657 PubMed29844306

2018

S100A11 protects against neuronal cell apoptosis induced by cerebral ischemia via inhibiting the nuclear translocation of annexin A1.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Xia,Xing Li,Huijuan Zhou,Lu Zheng,Jing Shi
View all publications
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Product promise

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