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AB171936

Anti-LDB3 antibody [EPR10126]

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(2 Publications)

Rabbit Recombinant Monoclonal LDB3 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

KIAA0613, ZASP, LDB3, LIM domain-binding protein 3, Protein cypher, Z-band alternatively spliced PDZ-motif protein

1 Images
Western blot - Anti-LDB3 antibody [EPR10126] (AB171936)
  • WB

Supplier Data

Western blot - Anti-LDB3 antibody [EPR10126] (AB171936)

All lanes:

Western blot - Anti-LDB3 antibody [EPR10126] (ab171936) at 1/1000 dilution

Lane 1:

A431 cell line lysate at 10 µg

Lane 2:

HT 1080 cell line lysate at 10 µg

Secondary

All lanes:

Goat ant-rabbit HRP conjugated antibody at 1/500 dilution

Predicted band size: 77 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10126

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LDB3 also known as LIM domain-binding protein 3 or ZASP plays a mechanical role in muscle function. It is a protein of approximately 80-110 kDa depending on its various isoforms. LDB3 is mainly expressed in cardiac and skeletal muscle tissues where it localizes at the Z-lines within the sarcomeres. It interacts with other structural proteins to provide stability impacting muscle contraction and integrity.
Biological function summary

In the context of muscle function LDB3 is an essential component of the sarcomere where it participates in a protein complex that includes alpha-actinin. This association helps maintain the structural organization of muscles and supports the force transmission required for muscle contractility. Furthermore LDB3 regulates protein-protein interactions contributing to muscle architecture and signaling pathways.

Pathways

The LDB3 protein operates in the context of muscle cell signaling and structural pathways. LDB3 connects to the integrin-linked kinase (ILK) pathway which influences cell survival and muscle differentiation. It also plays a role in the actin cytoskeleton pathway where it associates with proteins such as filamin and myotilin supporting muscle cell stability and dynamics.

LDB3 has significant connections to cardiomyopathy and myofibrillar myopathy. Mutations or deficiencies in LDB3 can lead to these conditions affecting muscle function and leading to symptoms such as muscle weakness or cardiac complications. LDB3 disorders can involve interactions with proteins like desmin which when mutated exacerbate muscle tissue disorganization and disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May function as an adapter in striated muscle to couple protein kinase C-mediated signaling via its LIM domains to the cytoskeleton.
See full target information LDB3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:270 PubMed36609526

2023

Expression of LIM domain-binding 3 (LDB3), a striated muscle Z-band alternatively spliced PDZ-motif protein in the nervous system.

Applications

Unspecified application

Species

Unspecified reactive species

Yotam Blech-Hermoni,Kalpana Subedi,Maya Silver,Leah Jensen,Stephen Coscia,Malcolm M Kates,Yongmei Zhao,Castle Raley,Nancy Edwards,Bao Tran,Abhik Ray-Chaudhary,Pankaj Pathak,Ami Mankodi

PLoS pathogens 18:e1010743 PubMed36067236

2022

Elucidation of TRIM25 ubiquitination targets involved in diverse cellular and antiviral processes.

Applications

Unspecified application

Species

Unspecified reactive species

Emily Yang,Serina Huang,Yasaman Jami-Alahmadi,Gerald M McInerney,James A Wohlschlegel,Melody M H Li
View all publications
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Product promise

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