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AB249656

Anti-LDB3 antibody [EPR10126] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal LDB3 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.
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Western blot - Anti-LDB3 antibody [EPR10126] - BSA and Azide free (AB249656)
  • WB

Supplier Data

Western blot - Anti-LDB3 antibody [EPR10126] - BSA and Azide free (AB249656)

This data was developed using ab171936, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LDB3 antibody [EPR10126] (<a href='/en-us/products/primary-antibodies/ldb3-antibody-epr10126-ab171936'>ab171936</a>) at 1/1000 dilution

Lane 1:

A431 cell line lysate at 10 µg

Lane 2:

HT 1080 cell line lysate at 10 µg

Secondary

All lanes:

Goat ant-rabbit HRP conjugated antibody at 1/500 dilution

Predicted band size: 77 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10126

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249656 is the carrier-free version of ab171936.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LDB3 also known as LIM domain-binding protein 3 or ZASP plays a mechanical role in muscle function. It is a protein of approximately 80-110 kDa depending on its various isoforms. LDB3 is mainly expressed in cardiac and skeletal muscle tissues where it localizes at the Z-lines within the sarcomeres. It interacts with other structural proteins to provide stability impacting muscle contraction and integrity.
Biological function summary

In the context of muscle function LDB3 is an essential component of the sarcomere where it participates in a protein complex that includes alpha-actinin. This association helps maintain the structural organization of muscles and supports the force transmission required for muscle contractility. Furthermore LDB3 regulates protein-protein interactions contributing to muscle architecture and signaling pathways.

Pathways

The LDB3 protein operates in the context of muscle cell signaling and structural pathways. LDB3 connects to the integrin-linked kinase (ILK) pathway which influences cell survival and muscle differentiation. It also plays a role in the actin cytoskeleton pathway where it associates with proteins such as filamin and myotilin supporting muscle cell stability and dynamics.

LDB3 has significant connections to cardiomyopathy and myofibrillar myopathy. Mutations or deficiencies in LDB3 can lead to these conditions affecting muscle function and leading to symptoms such as muscle weakness or cardiac complications. LDB3 disorders can involve interactions with proteins like desmin which when mutated exacerbate muscle tissue disorganization and disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May function as an adapter in striated muscle to couple protein kinase C-mediated signaling via its LIM domains to the cytoskeleton.
See full target information LDB3

Alternative Names

KIAA0613, ZASP, LDB3, LIM domain-binding protein 3, Protein cypher, Z-band alternatively spliced PDZ-motif protein
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Publications (1)

Recent publications for all applications. Explore the full listand refine your search

Oxidative medicine and cellular longevity 2021:8379962 PubMed34630853

2021

circ_0023461 Silencing Protects Cardiomyocytes from Hypoxia-Induced Dysfunction through Targeting miR-370-3p/PDE4D Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Ren,Buying Li,Liqing Jiang,Zhiheng Liu,Fan Wu,Yi Zhang,Jincheng Liu,Weixun Duan
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com