AB75999

Anti-LDH-C antibody [EP1567Y]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal LDHC antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

LDH3, LDHX, LDHC, L-lactate dehydrogenase C chain, LDH-C, Cancer/testis antigen 32, LDH testis subunit, LDH-X, CT32

1 Images

Unknown

Western blot - Anti-LDH-C antibody [EP1567Y] (AB75999)

All lanes:

Western blot - Anti-LDH-C antibody [EP1567Y] (ab75999) at 1/5000 dilution

All lanes:

human testis lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti rabbit at 1/2000 dilution

Predicted band size: 36 kDa

Observed band size: 35 kDa

false

Carrier free

Anti-LDH-C antibody [EP1567Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1567Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LDH-C also known as lactate dehydrogenase C is an enzyme belonging to the LDH family consisting of five similar proteins: LDH-A LDH-B LDH-C LDH-D and LDH-E. LDH-C displays a monomeric mass of about 36 kDa and primarily localizes in the testis. This enzyme catalyzes the reversible conversion of pyruvate to lactate with NADH serving as a cofactor. The kinetic properties and substrate affinities of LDH-C make it distinct from the other LDH isoforms found in various tissues improving its efficiency in the testes.

Biological function summary

Despite LDH-C being expressed much more in spermatozoa than in other tissues it plays a significant role in energy metabolism during spermatogenesis. It acts as part of the tetrameric LDH complex often in combination with other LDH isoforms. This complex facilitates lactate production which sperm cells rely on for energy in low-oxygen environments. The LDH-C form provides metabolic efficiency necessary for sperm motility and function.

Pathways

LDH-C is a critical participant in the glycolytic pathway which plays a central role in energy production under anaerobic conditions. LDH-C's role in converting pyruvate to lactate becomes essential for maintaining the balance of NAD+/NADH an important factor for glycolysis continuity. This enzyme also shares common pathways with LDH-A and LDH-B both important for similar metabolic functions but in other tissues like muscle and liver.

Researchers have noted associations between LDH-C expression levels and male infertility. Abnormal LDH-C forms or its dysfunctional activity may lead to decreased sperm motility or other sperm defects resulting in fertility issues. While no direct connection to other proteins in disease contexts currently known the overlap in function with LDH isoforms particularly LDH-A and LDH-B suggests that altered metabolic environments may influence a range of metabolic disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Possible role in sperm motility.

See full target information LDHC

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of immunopathology and pharm 30:25-43 PubMed28124574

2017

Curcumin regulates insulin pathways and glucose metabolism in the brains of APPswe/PS1dE9 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Pengwen Wang,Caixin Su,Huili Feng,Xiaopei Chen,Yunfang Dong,Yingxue Rao,Ying Ren,Jinduo Yang,Jing Shi,Jinzhou Tian,Shucui Jiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LDH-C antibody [EP1567Y]

Western blot - Anti-LDH-C antibody [EP1567Y] (AB75999)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise