Rabbit Monoclonal LDHC antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-LDH-C antibody [EP1746Y] - BSA and Azide free (AB284694), expandable thumbnail](https://content.abcam.com/products/images/ldh-c-antibody-ep1746y-bsa-and-azide-free-ab284694--western-blot-img169063.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LDH-C antibody [EP1746Y] - BSA and Azide free (AB284694)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDH-C antibody [EP1746Y] - BSA and Azide free (AB284694), expandable thumbnail](https://content.abcam.com/products/images/ldh-c-antibody-ep1746y-bsa-and-azide-free-ab284694--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img165108.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDH-C antibody [EP1746Y] - BSA and Azide free (AB284694)")
![Flow Cytometry (Intracellular) - Anti-LDH-C antibody [EP1746Y] - BSA and Azide free (AB284694), expandable thumbnail](https://content.abcam.com/products/images/ldh-c-antibody-ep1746y-bsa-and-azide-free-ab284694--flow-cytometry-intracellular-img170325.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-LDH-C antibody [EP1746Y] - BSA and Azide free (AB284694)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Tested | Tested |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Alternative names
LDH3, LDHX, LDHC, L-lactate dehydrogenase C chain, LDH-C, Cancer/testis antigen 32, LDH testis subunit, LDH-X, CT32
Recommended products
Rabbit Monoclonal LDHC antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP1746Y
- Purity
- Tissue culture supernatant
- Specificity
This antibody reacts with Lactate Dehydrogenase C / LDH-C. The immunogen sequence is 100% identical to Lactate Dehydrogenase C / LDH-C, but there is the possibility of cross-reactivity for lactate dehydrogenase A and lactate dehydrogenase B at 69% and 82%, respectively.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab284694 is the carrier-free version of Anti-LDH-C antibody [EP1746Y] ab52747
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LDH-C also known as lactate dehydrogenase C is an enzyme belonging to the LDH family consisting of five similar proteins: LDH-A LDH-B LDH-C LDH-D and LDH-E. LDH-C displays a monomeric mass of about 36 kDa and primarily localizes in the testis. This enzyme catalyzes the reversible conversion of pyruvate to lactate with NADH serving as a cofactor. The kinetic properties and substrate affinities of LDH-C make it distinct from the other LDH isoforms found in various tissues improving its efficiency in the testes.
- Biological function summary
Despite LDH-C being expressed much more in spermatozoa than in other tissues it plays a significant role in energy metabolism during spermatogenesis. It acts as part of the tetrameric LDH complex often in combination with other LDH isoforms. This complex facilitates lactate production which sperm cells rely on for energy in low-oxygen environments. The LDH-C form provides metabolic efficiency necessary for sperm motility and function.
- Pathways
LDH-C is a critical participant in the glycolytic pathway which plays a central role in energy production under anaerobic conditions. LDH-C's role in converting pyruvate to lactate becomes essential for maintaining the balance of NAD+/NADH an important factor for glycolysis continuity. This enzyme also shares common pathways with LDH-A and LDH-B both important for similar metabolic functions but in other tissues like muscle and liver.
- Associated diseases and disorders
Researchers have noted associations between LDH-C expression levels and male infertility. Abnormal LDH-C forms or its dysfunctional activity may lead to decreased sperm motility or other sperm defects resulting in fertility issues. While no direct connection to other proteins in disease contexts currently known the overlap in function with LDH isoforms particularly LDH-A and LDH-B suggests that altered metabolic environments may influence a range of metabolic disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-LDH-C antibody [EP1746Y] - BSA and Azide free (ab284694), expandable thumbnail](https://content.abcam.com/products/images/ldh-c-antibody-ep1746y-bsa-and-azide-free-ab284694--western-blot-img169063.jpg "Western blot - Anti-LDH-C antibody [EP1746Y] - BSA and Azide free (ab284694)")
Western blot - Anti-LDH-C antibody [EP1746Y] - BSA and Azide free (ab284694)
This data was developed using Anti-LDH-C antibody [EP1746Y] ab52747, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-LDH-C antibody [EP1746Y] - BSA and Azide free (ab284694) at 1/20000 dilution
All lanes: Human testis lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 36 kDa
Observed band size: 31 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDH-C antibody [EP1746Y] - BSA and Azide free (ab284694), expandable thumbnail](https://content.abcam.com/products/images/ldh-c-antibody-ep1746y-bsa-and-azide-free-ab284694--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img165108.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDH-C antibody [EP1746Y] - BSA and Azide free (ab284694)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDH-C antibody [EP1746Y] - BSA and Azide free (ab284694)
This data was developed using Anti-LDH-C antibody [EP1746Y] ab52747, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin-embedded human testis carcinoma using Anti-LDH-C antibody [EP1746Y] ab52747 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-LDH-C antibody [EP1746Y] - BSA and Azide free (ab284694), expandable thumbnail](https://content.abcam.com/products/images/ldh-c-antibody-ep1746y-bsa-and-azide-free-ab284694--flow-cytometry-intracellular-img170325.jpg "Flow Cytometry (Intracellular) - Anti-LDH-C antibody [EP1746Y] - BSA and Azide free (ab284694)")
Flow Cytometry (Intracellular) - Anti-LDH-C antibody [EP1746Y] - BSA and Azide free (ab284694)
This data was developed using Anti-LDH-C antibody [EP1746Y] ab52747, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of SK-BR-3 (Human breast adenocarcinoma epithelial cell) cells labeling LDH-C with Anti-LDH-C antibody [EP1746Y] ab52747 at 1/40 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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