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AB250588

Anti-LDHD/DLD antibody [EPR15068(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal LDHD/DLD antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

DLD, Lactate dehydrogenase D, LDHD

1 Images
Western blot - Anti-LDHD/DLD antibody [EPR15068(B)] - BSA and Azide free (AB250588)
  • WB

Supplier Data

Western blot - Anti-LDHD/DLD antibody [EPR15068(B)] - BSA and Azide free (AB250588)

This data was developed using ab182146, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LDHD/DLD antibody [EPR15068(B)] (<a href='/en-us/products/primary-antibodies/ldhd-dld-antibody-epr15068b-ab182146'>ab182146</a>) at 1/2000 dilution

Lane 1:

Human fetal liver lysate at 20 µg

Lane 2:

Human fetal heart lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 55 kDa

Observed band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15068(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250588 is the carrier-free version of ab182146.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The LDHD/DLD protein also known as D-lactate dehydrogenase and capable of forming a complex with DLD (dihydrolipoamide dehydrogenase) has a molecular mass approximately 60 kDa. It mainly functions as an enzyme responsible for catalyzing the conversion of D-lactate to pyruvate in the mitochondria. Expression of LDHD is primarily observed in metabolic tissues such as liver kidney and heart. This protein plays an important role in energy production and metabolism by contributing to cellular redox balance.
Biological function summary

LDHD/DLD has an essential role in the mitochondrial energy metabolism through its involvement in the D-lactate metabolism. While LDHD/DLD itself is not formally a part of the pyruvate dehydrogenase complex DLD present in the complex shares the enzymatic responsibility for electron transfer needed for efficient energy conversion. The enzymatic action of LDHD in concert with DLD maintains cellular energy balance and supports effective metabolic processes.

Pathways

Research has shown connections between LDHD/DLD activity and the pyruvate metabolism and glycolysis pathways. In these pathways LDHD/DLD helps in channeling D-lactate derived electrons into the electron transport chain facilitating energy production in the form of ATP. The interaction between LDHD and proteins like pyruvate dehydrogenase ties them to those pathways ensuring proper cellular respiration and metabolic regulation within cells that demand high energy.

LDHD/DLD has been linked to issues involving lactic acidosis and some metabolic syndromes. A dysfunction in the enzymatic activity of LDHD/DLD could lead to the accumulation of D-lactate contributing to lactic acidosis which disrupts normal pH balance. Disorders in metabolic pathways involving DLD and related proteins can also manifest in altered energy metabolism linking LDHD/DLD to conditions like mitochondrial disorders and some forms of diabetes. Understanding the enzymatic roles of LDHD/DLD helps in devising therapeutic approaches for these metabolic diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in D-lactate, but not L-lactate catabolic process.
See full target information LDHD
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com