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AB277134

Anti-LDL Receptor antibody [032] - BSA and Azide free

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Rabbit Recombinant Monoclonal LDL Receptor antibody. Carrier free. Suitable for ICC, Flow Cyt and reacts with Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Low-density lipoprotein receptor aa 1-800.

View Alternative Names

Low-density lipoprotein receptor, LDL receptor, Ldlr

2 Images
Flow Cytometry - Anti-LDL Receptor antibody [032] - BSA and Azide free (AB277134)
  • Flow Cyt

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Flow Cytometry - Anti-LDL Receptor antibody [032] - BSA and Azide free (AB277134)

Flow cytometric analysis of LDL Receptor expression on Raw264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells. The cells were stained with ab277134 at 1/25 dilution, then a FITC-conjugated second step antibody (Black). Cells were also stained with an isotype control (Grey). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Immunocytochemistry - Anti-LDL Receptor antibody [032] - BSA and Azide free (AB277134)
  • ICC

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Immunocytochemistry - Anti-LDL Receptor antibody [032] - BSA and Azide free (AB277134)

Immunofluorescence staining of LDL Receptor in Raw264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with ab277134 at 1/100 at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope) Goat Anti-rabbit IgG secondary antibody(green), counterstained with DAPI (blue).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

032

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

Flow Cyt, ICC

applications

Immunogen

Recombinant Fragment Protein within Mouse Low-density lipoprotein receptor aa 1-800. The exact immunogen used to generate this antibody is proprietary information.

P35951

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/20 - 1/100", "ICC-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/25 - 1/100", "FlowCyt-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The LDL Receptor also called LDLR is a protein that plays an important role in the uptake and clearance of low-density lipoproteins (LDL) from the bloodstream. LDLR binds LDL particles facilitating their internalization through receptor-mediated endocytosis. This receptor is expressed mainly in the liver adrenal glands and other tissues involved in cholesterol metabolism. It has an approximate molecular weight of 160 kDa. Researchers often study LDLR using techniques like Western blotting to understand its presence and function.
Biological function summary

The LDL receptor interacts with LDL particles to regulate cholesterol levels in the body. It is part of a cell surface complex that recognizes and binds to apolipoprotein B-100 or apolipoprotein E present on LDL. This interaction initiates internalization of LDL leading to its degradation in lysosomes where cholesterol can be released and used by the cell. Mutations in the gene encoding LDLR can lead to inefficient cholesterol uptake influencing various metabolic processes.

Pathways

LDL receptor activities are integral to lipid metabolism and cholesterol homeostasis. Two important biological pathways that involve LDLR include the cholesterol biosynthesis pathway and the lipoprotein clearance pathway. Within these pathways LDLR collaborates closely with proteins like PCSK9 which modulates its expression and degradation and HMG-CoA reductase an important enzyme in cholesterol synthesis to balance cholesterol levels in the body.

Defects in the LDL receptor are strongly associated with familial hypercholesterolemia and atherosclerosis. These conditions arise from impaired clearance of LDL leading to elevated cholesterol levels which pose risks for cardiovascular diseases. LDLR dysfunctions are linked with the Protein PCSK9 whose gain-of-function mutations can exacerbate hypercholesterolemia by promoting degradation of LDLR while statins aim to increase LDLR expression to lower LDL cholesterol levels.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds low density lipoprotein /LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. Forms a ternary complex with PGRMC1 and TMEM97 receptors which increases LDLR-mediated LDL internalization.
See full target information Low-density lipoprotein receptor
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