Anti-LDL Receptor antibody [2B10]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LDL Receptor antibody [2B10] (AB314008)

Immunofluorescence staining of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells with ab314008 at 1 : 50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-LDL Receptor antibody [2B10] (AB314008)

Overlay histogram showing HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained with ab314008 (pink line) at 1 : 50. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1 x 10E6 cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1μg/1 x 10E6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

• WB

Supplier Data

Western blot - Anti-LDL Receptor antibody [2B10] (AB314008)

All lanes:

Western blot - Anti-LDL Receptor antibody [2B10] (ab314008) at 1/1000 dilution

Lane 1:

Hela whole cell lysate at 30 µg

Lane 2:

JK whole cell lysate at 30 µg

Lane 3:

SY5Y whole cell lysate at 30 µg

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/20000 dilution

Predicted band size: 96 kDa

Observed band size: 100 kDa

false

Exposure time: 60s