Anti-LDL Receptor antibody

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDL Receptor antibody (AB30532)

Immunohistochemistry analysis of formalin-fixed, paraffin-embedded (FFPE) mouse lung tissue after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with LDL receptor polyclonal antibody, ab30532, at a 1 : 100 dilution, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen (DAB).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-LDL Receptor antibody (AB30532)

ICC/IF image of ab30532 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30532, 1/400) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDL Receptor antibody (AB30532)

ab30532 staining LDL receptor in human lung parenchyma.
Left panel : with primary antibody at 1/400. Right panel : isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature : sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

• ICC

AbReview80948****

Immunocytochemistry - Anti-LDL Receptor antibody (AB30532)

Immunocytochemistry analysis of acetone-fixed human Liver cells, Hep2G staining with ab30532 at 1/200 dilution. Secondary antibody was FITC rabbit IgG at 1/400 dilution. Samples were incubated with the primary antibody with Serum/PBS for 1 hours at 21°C. Blocking was done using 5% serum for 15 minutes at 21°C.

This image is courtesy of an anonymous Abreview

• WB

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Western blot - Anti-LDL Receptor antibody (AB30532)

All lanes:

Western blot - Anti-LDL Receptor antibody (ab30532)

Lane 1:

HeLa Cell Lysate at 50 µg

Lane 2:

HepG2 Cell Lysate at 50 µg

Lane 3:

Huh7 Cell Lysate at 50 µg

Predicted band size: 95 kDa

false

• WB

Supplier Data

Western blot - Anti-LDL Receptor antibody (AB30532)

All lanes:

Western blot - Anti-LDL Receptor antibody (ab30532) at 1/200 dilution

Lane 1:

RAW 264.7 Cells at 25 µg

Lane 2:

RAW 264.7 Cells at 50 µg

Lane 3:

Rat Liver Supernatant at 50 µg

Secondary

All lanes:

Anti-Rabbit-IgG-HRP at 1/7000 dilution

Predicted band size: 95 kDa

true