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Rabbit Recombinant Monoclonal LEC antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

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Images

Sandwich ELISA - Anti-LEC antibody [EPR21737-212] - BSA and Azide free (Detector) (AB259508), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Expected

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Shows chemotactic activity for lymphocytes and monocytes but not neutrophils. Also shows potent myelosuppressive activity, suppresses proliferation of myeloid progenitor cells. Recombinant SCYA16 shows chemotactic activity for monocytes and THP-1 monocytes, but not for resting lymphocytes and neutrophils. Induces a calcium flux in THP-1 cells that were desensitized by prior expression to RANTES.

Alternative names

ILINCK, NCC4, SCYA16, CCL16, C-C motif chemokine 16, Chemokine CC-4, Chemokine LEC, IL-10-inducible chemokine, LCC-1, Liver-expressed chemokine, Lymphocyte and monocyte chemoattractant, Monotactin-1, NCC-4, Small-inducible cytokine A16, HCC-4, LMC, MTN-1

Recommended products

Rabbit Recombinant Monoclonal LEC antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR21737-212
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab259508 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human CCL16. The recommended pair for sandwich ELISA is:
Capture: Anti-LEC antibody [EPR21737-236] - BSA and Azide free (Capture) ab259507, Human CCL16 Capture Antibody (unconjugated)
Detector: ab259508, Human CCL16 Detector Antibody (unconjugated)
The reference range value is 2.34 - 150 pg/ml.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

LEC also known as CCL16 is a chemokine protein that functions in the immune system. This protein weighs approximately 22 kDa and resides mainly in the liver and peripheral blood leukocytes. LEC belongs to the CC chemokine family characterized by two adjacent cysteine residues. The protein connects with other cellular systems to modulate immune responses. Antibodies like anti-LEC can detect it effectively and commercial products such as LEC BSA and LEC custom products aid in its study.

Biological function summary

LEC performs vital roles in immune cell signaling and chemotaxis. It binds specifically to certain chemokine receptors on target cells initiating signaling cascades that recruit lymphocytes to infected or inflamed tissues. LEC functions as part of a larger complex that includes other chemokines to coordinate immune responses. Its expression and activity influence various immune functions which makes its regulation significant for maintaining healthy immune system operation.

Pathways

LEC acts within immune signaling pathways and inflammation-related processes. It plays essential parts in pathways like the chemokine signaling pathway where it interacts with other proteins such as CCR1 and CCR2 chemokine receptors. Through these interactions LEC contributes to the recruitment and activation of specific immune cell subtypes facilitating a coordinated response to inflammation or infection.

Associated diseases and disorders

LEC connects to conditions involving immune dysregulation like rheumatoid arthritis and liver diseases. In rheumatoid arthritis LEC levels can affect inflammation and tissue damage often in concert with proteins like TNF-alpha. In liver diseases altered LEC expression may relate to liver fibrosis with connections to proteins such as TGF-beta. Understanding LEC's role in these conditions can lead to better therapeutic strategies targeting the chemokine network.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Anti-LEC antibody [EPR21737-212] - BSA and Azide free (Detector) (ab259508), expandable thumbnail

    Sandwich ELISA - Anti-LEC antibody [EPR21737-212] - BSA and Azide free (Detector) (ab259508)

    Representative standard curve from corresponding SimpleStep ELISA® Kit (Human CCL16 ELISA Kit ab243673).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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