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AB248727

Anti-LEC antibody [EPR4452(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal LEC antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

ILINCK, NCC4, SCYA16, CCL16, C-C motif chemokine 16, Chemokine CC-4, Chemokine LEC, IL-10-inducible chemokine, LCC-1, Liver-expressed chemokine, Lymphocyte and monocyte chemoattractant, Monotactin-1, NCC-4, Small-inducible cytokine A16, HCC-4, LMC, MTN-1

2 Images
Western blot - Anti-LEC antibody [EPR4452(2)] - BSA and Azide free (AB248727)
  • WB

Unknown

Western blot - Anti-LEC antibody [EPR4452(2)] - BSA and Azide free (AB248727)

This data was developed using ab134917, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LEC antibody [EPR4452(2)] (<a href='/en-us/products/primary-antibodies/lec-antibody-epr44522-ab134917'>ab134917</a>) at 1/10000 dilution

All lanes:

Human LEC recombinant protein (10ng)

Secondary

All lanes:

Secondary Goat-anti-rabbit HRP at 1/2000 dilution

Predicted band size: 14 kDa

true

OI-RD Scanning - Anti-LEC antibody [EPR4452(2)] - BSA and Azide free (AB248727)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-LEC antibody [EPR4452(2)] - BSA and Azide free (AB248727)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4452(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab248727 is the carrier-free version of ab134917.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LEC also known as CCL16 is a chemokine protein that functions in the immune system. This protein weighs approximately 22 kDa and resides mainly in the liver and peripheral blood leukocytes. LEC belongs to the CC chemokine family characterized by two adjacent cysteine residues. The protein connects with other cellular systems to modulate immune responses. Antibodies like anti-LEC can detect it effectively and commercial products such as LEC BSA and LEC custom products aid in its study.
Biological function summary

LEC performs vital roles in immune cell signaling and chemotaxis. It binds specifically to certain chemokine receptors on target cells initiating signaling cascades that recruit lymphocytes to infected or inflamed tissues. LEC functions as part of a larger complex that includes other chemokines to coordinate immune responses. Its expression and activity influence various immune functions which makes its regulation significant for maintaining healthy immune system operation.

Pathways

LEC acts within immune signaling pathways and inflammation-related processes. It plays essential parts in pathways like the chemokine signaling pathway where it interacts with other proteins such as CCR1 and CCR2 chemokine receptors. Through these interactions LEC contributes to the recruitment and activation of specific immune cell subtypes facilitating a coordinated response to inflammation or infection.

LEC connects to conditions involving immune dysregulation like rheumatoid arthritis and liver diseases. In rheumatoid arthritis LEC levels can affect inflammation and tissue damage often in concert with proteins like TNF-alpha. In liver diseases altered LEC expression may relate to liver fibrosis with connections to proteins such as TGF-beta. Understanding LEC's role in these conditions can lead to better therapeutic strategies targeting the chemokine network.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Shows chemotactic activity for lymphocytes and monocytes but not neutrophils. Also shows potent myelosuppressive activity, suppresses proliferation of myeloid progenitor cells. Recombinant SCYA16 shows chemotactic activity for monocytes and THP-1 monocytes, but not for resting lymphocytes and neutrophils. Induces a calcium flux in THP-1 cells that were desensitized by prior expression to RANTES.
See full target information CCL16
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Product promise

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