Rabbit Polyclonal LEF1 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human LEF1_HUMAN.
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- Communications biology 5:10182022Akt/mTOR integrate energy metabolism with Wnt signal to influence wound epithelium growth in Gekko Japonicus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQinghua Wang,Zuming Mao,Zhuang Liu,Man Xu,Shuai Huang,Yin Wang,Yanran Xu,Longju Qi,Mei Liu,Yan LiuPubMed 36167813
- Cell death discovery 8:462022NR4A1 promotes LEF1 expression in the pathogenesis of papillary thyroid cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCen Jiang,Jianli He,Sunwang Xu,Qi Wang,Jinke ChengPubMed 35110542
- Science signaling 11:2018The transcription factor Lef1 switches partners from β-catenin to Smad3 during muscle stem cell quiescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAjoy Aloysius et. al.PubMed 30042129
- Journal of cellular physiology 233:3418-34282017Regulating osteogenesis and adipogenesis in adipose-derived stem cells by controlling underlying substrate stiffness.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Zhang,Shiyu Lin,Xiaoru Shao,Sirong Shi,Qi Zhang,Changyue Xue,Yunfeng Lin,Bofeng Zhu,Xiaoxiao CaiPubMed 28926111
- Molecular medicine reports 14:1891-9002016Disruption of kif3a results in defective osteoblastic differentiation in dental mesenchymal stem/precursor cells via the Wnt signaling pathway.
- Cell cycle (Georgetown, Tex.) 14:3396-4072015Tumorigenicity analysis of heterogeneous dental stem cells and its self-modification for chromosome instability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhaosong Meng,Guoqing Chen,Jinlong Chen,Bo Yang,Mei Yu,Lian Feng,Zongting Jiang,Weihua Guo,Weidong TianPubMed 26322910
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 2% BSA- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human LEF1_HUMAN. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UJU2
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Rat | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 - 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
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Target data
Function
Transcription factor that binds DNA in a sequence-specific manner (PubMed:2010090). Participates in the Wnt signaling pathway (By similarity). Activates transcription of target genes in the presence of CTNNB1 and EP300 (By similarity). PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1 (PubMed:11266540). Regulates T-cell receptor alpha enhancer function (PubMed:19653274). Required for IL17A expressing gamma-delta T-cell maturation and development, via binding to regulator loci of BLK to modulate expression (By similarity). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, expression is repressed during the bell stage by MSX1-mediated inhibition of CTNNB1 signaling (By similarity). May play a role in hair cell differentiation and follicle morphogenesis (By similarity). Isoform 1. Transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells. Isoform 3. Lacks the CTNNB1 interaction domain and may therefore be an antagonist for Wnt signaling. Isoform 5. Transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells.
Alternative names
Lymphoid enhancer-binding factor 1, LEF-1, T cell-specific transcription factor 1-alpha, TCF1-alpha, LEF1
Recommended products
Rabbit Polyclonal LEF1 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human LEF1_HUMAN.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 2% BSA- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human LEF1_HUMAN. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UJU2
- Purification technique
- Affinity purification Immunogen
- Reconstitution
- reconstitute with water at 200µL
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LEF1 also known as lymphoid enhancer-binding factor 1 is a transcription factor important in the Wnt signaling pathway. It typically weighs around 44 kDa. This protein contains a high-mobility group (HMG) box domain that enables it to bind specifically to DNA and influence gene expression. LEF1 is primarily expressed in tissues with active Wnt signaling including developing organs and certain cell types like T-cells specifically Jurkat cells. Researchers use LEF1 protein immunohistochemistry (LEF1 IHC) and LEF1 markers for expression analysis in different tissues.
- Biological function summary
The LEF1 transcription factor plays a significant role in cellular processes such as cell differentiation proliferation and embryonic development. As part of the Wnt signaling complex it interacts with β-catenin to regulate target gene expression. Activation of Wnt signaling by LEF1 can lead to changes in the cellular structure and function necessary for tissue development and regeneration. LEF1 also contributes to maintaining the pluripotency of stem cells by modulating gene networks critical for stem cell properties.
- Pathways
The LEF1 protein is essential in Wnt/β-catenin signaling and influences the Hedgehog pathway. LEF1 pairs with β-catenin to drive the transcription of Wnt target genes involved in cell cycle and differentiation. These pathways maintain stem cell populations and regulate development. LEF1's function in these pathways links it closely to other factors like TCF7L2 and GLI family proteins which also play vital roles within these signaling cascades.
- Associated diseases and disorders
LEF1 can be linked to conditions such as cancer and immune-related diseases. Overexpression or mutation of the LEF1 protein can lead to various types of cancers including leukemia and colorectal cancer by promoting abnormal cell proliferation and survival. LEF1's role in the immune system also associates it with autoimmune diseases where dysregulation of Wnt signaling might contribute to disease progression. Altered interactions between LEF1 and β-catenin or other associated proteins can disrupt normal cellular activities leading to these pathologies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-LEF1 antibody (ab85052)
All lanes: Western blot - Anti-LEF1 antibody (ab85052) at 1/500 dilution
Lane 1: K562 cell lysate
Lane 2: Jurkat cell lysate
Predicted band size: 44 kDa
Observed band size: 44 kDa
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