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AB85052

Anti-LEF1 antibody

5

(3 Reviews)

|

(6 Publications)

Rabbit Polyclonal LEF1 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human LEF1_HUMAN.

View Alternative Names

Lymphoid enhancer-binding factor 1, LEF-1, T cell-specific transcription factor 1-alpha, TCF1-alpha, LEF1

1 Images
Western blot - Anti-LEF1 antibody (AB85052)
  • WB

Unknown

Western blot - Anti-LEF1 antibody (AB85052)

All lanes:

Western blot - Anti-LEF1 antibody (ab85052) at 1/500 dilution

Lane 1:

K562 cell lysate

Lane 2:

Jurkat cell lysate

Predicted band size: 44 kDa

Observed band size: 44 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human LEF1_HUMAN. The exact immunogen used to generate this antibody is proprietary information.

Q9UJU2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 200µL
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.02% Sodium azide Constituents: 2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LEF1 also known as lymphoid enhancer-binding factor 1 is a transcription factor important in the Wnt signaling pathway. It typically weighs around 44 kDa. This protein contains a high-mobility group (HMG) box domain that enables it to bind specifically to DNA and influence gene expression. LEF1 is primarily expressed in tissues with active Wnt signaling including developing organs and certain cell types like T-cells specifically Jurkat cells. Researchers use LEF1 protein immunohistochemistry (LEF1 IHC) and LEF1 markers for expression analysis in different tissues.
Biological function summary

The LEF1 transcription factor plays a significant role in cellular processes such as cell differentiation proliferation and embryonic development. As part of the Wnt signaling complex it interacts with β-catenin to regulate target gene expression. Activation of Wnt signaling by LEF1 can lead to changes in the cellular structure and function necessary for tissue development and regeneration. LEF1 also contributes to maintaining the pluripotency of stem cells by modulating gene networks critical for stem cell properties.

Pathways

The LEF1 protein is essential in Wnt/β-catenin signaling and influences the Hedgehog pathway. LEF1 pairs with β-catenin to drive the transcription of Wnt target genes involved in cell cycle and differentiation. These pathways maintain stem cell populations and regulate development. LEF1's function in these pathways links it closely to other factors like TCF7L2 and GLI family proteins which also play vital roles within these signaling cascades.

LEF1 can be linked to conditions such as cancer and immune-related diseases. Overexpression or mutation of the LEF1 protein can lead to various types of cancers including leukemia and colorectal cancer by promoting abnormal cell proliferation and survival. LEF1's role in the immune system also associates it with autoimmune diseases where dysregulation of Wnt signaling might contribute to disease progression. Altered interactions between LEF1 and β-catenin or other associated proteins can disrupt normal cellular activities leading to these pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that binds DNA in a sequence-specific manner (PubMed : 2010090). Participates in the Wnt signaling pathway (By similarity). Activates transcription of target genes in the presence of CTNNB1 and EP300 (By similarity). PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1 (PubMed : 11266540). Regulates T-cell receptor alpha enhancer function (PubMed : 19653274). Required for IL17A expressing gamma-delta T-cell maturation and development, via binding to regulator loci of BLK to modulate expression (By similarity). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, expression is repressed during the bell stage by MSX1-mediated inhibition of CTNNB1 signaling (By similarity). May play a role in hair cell differentiation and follicle morphogenesis (By similarity).. Isoform 1. Transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.. Isoform 3. Lacks the CTNNB1 interaction domain and may therefore be an antagonist for Wnt signaling.. Isoform 5. Transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells.
See full target information LEF1_HUMAN
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 5:1018 PubMed36167813

2022

Akt/mTOR integrate energy metabolism with Wnt signal to influence wound epithelium growth in Gekko Japonicus.

Applications

Unspecified application

Species

Unspecified reactive species

Qinghua Wang,Zuming Mao,Zhuang Liu,Man Xu,Shuai Huang,Yin Wang,Yanran Xu,Longju Qi,Mei Liu,Yan Liu

Cell death discovery 8:46 PubMed35110542

2022

NR4A1 promotes LEF1 expression in the pathogenesis of papillary thyroid cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Cen Jiang,Jianli He,Sunwang Xu,Qi Wang,Jinke Cheng

Science signaling 11: PubMed30042129

2018

The transcription factor Lef1 switches partners from β-catenin to Smad3 during muscle stem cell quiescence.

Applications

Unspecified application

Species

Unspecified reactive species

Ajoy Aloysius,Ramanuj DasGupta,Jyotsna Dhawan

Journal of cellular physiology 233:3418-3428 PubMed28926111

2017

Regulating osteogenesis and adipogenesis in adipose-derived stem cells by controlling underlying substrate stiffness.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Zhang,Shiyu Lin,Xiaoru Shao,Sirong Shi,Qi Zhang,Changyue Xue,Yunfeng Lin,Bofeng Zhu,Xiaoxiao Cai

Molecular medicine reports 14:1891-900 PubMed27432616

2016

Disruption of kif3a results in defective osteoblastic differentiation in dental mesenchymal stem/precursor cells via the Wnt signaling pathway.

Applications

WB

Species

Human

Sicong Jiang,Guoqing Chen,Lian Feng,Zongting Jiang,Mei Yu,Jinku Bao,Weidong Tian

Cell cycle (Georgetown, Tex.) 14:3396-407 PubMed26322910

2015

Tumorigenicity analysis of heterogeneous dental stem cells and its self-modification for chromosome instability.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaosong Meng,Guoqing Chen,Jinlong Chen,Bo Yang,Mei Yu,Lian Feng,Zongting Jiang,Weihua Guo,Weidong Tian
View all publications
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