Skip to main content

Rabbit anti-LEF1 antibody EPR2029Y ab137872 is a rabbit monoclonal antibody that is used in LEF1 western blotting, IHC, immunofluorescence and flow cytometry. Suitable for human, mouse and rat samples.

Recombinant format for high batch-to-batch consistency and reproducible results
Specificity confirmed with Lef1 knockout cell line validation
Specificity and sensitivity confirmed in IHC with multi-tissue microarray validation.
Anti-LEF1 antibody clone EPR2029Y is cited in over 80 publications


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Expected
Expected
Rat
Tested
Tested
Tested
Expected
Expected

Tested
Tested

Species

Mouse

Dilution info

1/100 - 1/500

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/100 - 1/500

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/100 - 1/500

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Rat, Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/1000

Notes

We don't recommend this antibody for mouse in Western Blot. In our hands an extra band was observed in mouse tissue lysates.

Species

Human

Dilution info

1/1000

Notes

We don't recommend this antibody for mouse in Western Blot. In our hands an extra band was observed in mouse tissue lysates.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Transcription factor that binds DNA in a sequence-specific manner (PubMed:2010090). Participates in the Wnt signaling pathway (By similarity). Activates transcription of target genes in the presence of CTNNB1 and EP300 (By similarity). PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1 (PubMed:11266540). Regulates T-cell receptor alpha enhancer function (PubMed:19653274). Required for IL17A expressing gamma-delta T-cell maturation and development, via binding to regulator loci of BLK to modulate expression (By similarity). May play a role in hair cell differentiation and follicle morphogenesis (By similarity).Isoform 1Transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.Isoform 3Lacks the CTNNB1 interaction domain and may therefore be an antagonist for Wnt signaling.Isoform 5Transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells.

Alternative names

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Rabbit anti-LEF1 antibody EPR2029Y ab137872 is a rabbit monoclonal antibody that is used in LEF1 western blotting, IHC, immunofluorescence and flow cytometry. Suitable for human, mouse and rat samples.

Recombinant format for high batch-to-batch consistency and reproducible results
Specificity confirmed with Lef1 knockout cell line validation
Specificity and sensitivity confirmed in IHC with multi-tissue microarray validation.
Anti-LEF1 antibody clone EPR2029Y is cited in over 80 publications

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR2029Y

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

LEF1 plays a significant role in the regulation of cell fate decisions and development. It acts as part of a transcription complex that binds to specific DNA sequences facilitating the transcription of target genes. LEF1 interacts with ?-catenin and this interaction is essential for the regulation of gene expression during embryonic development and cellular proliferation. LEF1's biological functions make it a useful marker in various research applications and assays like LEF1 ELISA are often utilized to study its expression levels in different physiological contexts.

Activity summary

LEF1 (Lymphoid Enhancer-Binding Factor 1) also known as LEF-1 or LEF 1 functions as a transcription factor involved in Wnt signaling pathways. The LEF1 protein has a mass of about 45 kilodaltons. Expression of LEF1 occurs mainly in lymphoid tissues and the central nervous system. Researchers often use LEF1 in immunohistochemistry (LEF1 IHC) for its ability to bind DNA at specific sites regulating gene expression important for cellular development and differentiation especially in T cells such as Jurkat cells.

Pathways

LEF1 is a central player in the canonical Wnt/?-catenin signaling pathway. This pathway is fundamental for processes like embryogenesis and cellular growth. LEF1 interacts closely with proteins such as TCF (T-cell factor) family members and ?-catenin to regulate gene expression. Disruption in the Wnt pathway where LEF1 is significant can lead to abnormal cell growth and differentiation. Consequently LEF1 serves as an indicator and potential regulator of pathway activities in various cellular environments.

Associated diseases and disorders

LEF1 is associated with numerous conditions particularly in oncology and immunology. Aberrations in LEF1 expression contribute to diseases like leukemia and lymphoma. Overexpression or mutations of LEF1 might lead to unregulated Wnt signaling promoting cancer progression. Furthermore LEF1's interactions with proteins such as MYC a well-known oncogene highlight its importance in tumorigenesis. Understanding LEF1's mechanisms can offer insights into therapeutic targets for these diseases providing a pathway for research into drug development and intervention strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

20 product images

  • Immunoprecipitation - Anti-LEF1 antibody [EPR2029Y] (ab137872), expandable thumbnail

    Immunoprecipitation - Anti-LEF1 antibody [EPR2029Y] (ab137872)

    Lane 1 (input): Jurkat (human T cell leukemia T lymphocyte) whole cell lysate, 10 μg
    Lane 2 (+): Jurkat whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab137872 in Jurkat whole cell lysate

    ab137872 immunoprecipitating LEF1 in Jurkat whole cell lysate. For western blotting, primary antibody used was ab137872 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Blocking and diluting buffer: 5% NFDM/TBST

    Exposure time: 3 minutes

    All lanes: Immunoprecipitation - Anti-LEF1 antibody [EPR2029Y] (AB137872)

    Predicted band size: 44 kDa

    Exposure time: 3min

  • Western blot - Anti-LEF1 antibody [EPR2029Y] (ab137872), expandable thumbnail

    Western blot - Anti-LEF1 antibody [EPR2029Y] (ab137872)

    Blocking/Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-LEF1 antibody [EPR2029Y] (AB137872) at 1/1000 dilution

    All lanes: Human fetal thymus lysate at 10 µg

    Secondary

    All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 44 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 antibody [EPR2029Y] (ab137872), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 antibody [EPR2029Y] (ab137872)

    Immunohistochemical staining of paraffin embedded human tonsil with purified ab137872 at a working dilution of 1/500. The secondary antibody used is ab97051, an HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Western blot - Anti-LEF1 antibody [EPR2029Y] (ab137872), expandable thumbnail

    Western blot - Anti-LEF1 antibody [EPR2029Y] (ab137872)

    False colour image of Western blot: Anti-LEF1 antibody [EPR2029Y] staining at 1/1000 dilution, shown in black; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab137872 was shown to bind specifically to LEF1. A band was observed at 40/53 kDa in wild-type Jurkat cell lysates with no signal observed at this size in Lef1 knockout cell line ab274898 (knockout cell lysate ab274956). To generate this image, wild-type and Lef1 knockout Jurkat cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent ab133456) and imaged with 4 minutes exposure time. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-LEF1 antibody [EPR2029Y] (AB137872) at 1/1000 dilution

    Lane 1: Wild-type Jurkat cell lysate at 40 µg

    Lane 2: Lef1 knockout Jurkat cell lysate at 40 µg

    Lane 3: Jurkat cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 44 kDa

    Observed band size: 40 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-LEF1 antibody [EPR2029Y] (ab137872), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-LEF1 antibody [EPR2029Y] (ab137872)

    Immunofluorescence staining of Jurkat (Human T cell leukemia cell line from peripheral blood) cells with purified ab137872 at a working dilution of 1 in 500, counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120) . The secondary antibody was ab150077 Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100.

    The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified ab137872 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab15007) were used.

  • Flow Cytometry (Intracellular) - Anti-LEF1 antibody [EPR2029Y] (ab137872), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-LEF1 antibody [EPR2029Y] (ab137872)

    Intracellular flow cytometric analysis of Jurkat cell line (human T cell leukemia T lymphocyte) fixed with 4% paraformaldehyde and permeabilized with 90% methanol labeling LEF1 with ab137872 at 1/600 dilution (red). This is compared with a Rabbit monoclonal IgG (ab172730) - Isotype control (black) and a unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti-rabbit IgG (Alexa Fluor®488) was used as the secondary antibody.

  • Western blot - Anti-LEF1 antibody [EPR2029Y] (ab137872), expandable thumbnail

    Western blot - Anti-LEF1 antibody [EPR2029Y] (ab137872)

    Different batches of ab137872 were tested on Jurkat (Human T cell leukemia T lymphocyte) lysate at 1.1 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 25-57 kDa.

    All lanes: Western blot - Anti-LEF1 antibody [EPR2029Y] (AB137872)

    Predicted band size: 44 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 antibody [EPR2029Y] (ab137872), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 antibody [EPR2029Y] (ab137872)

    Immunohistochemical staining of paraffin embedded rat spleen with purified ab137872 at a working dilution of 1/500. The secondary antibody used is ab97051, an HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 antibody [EPR2029Y] (ab137872), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 antibody [EPR2029Y] (ab137872)

    Immunohistochemical staining of paraffin-embedded human thymus with purified ab137872 at a working dilution of 1/500. The secondary antibody used is ab97051, an HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunoprecipitation - Anti-LEF1 antibody [EPR2029Y] (ab137872), expandable thumbnail

    Immunoprecipitation - Anti-LEF1 antibody [EPR2029Y] (ab137872)

    Lane 1 (input): Rat thymus lysate, 10μg
    Lane 2 (+): Rat thymus lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab137872 in rat thymus lysate

    ab137872 immunoprecipitating LEF1 in rat thymus lysate. For western blotting, primary antibody used was ab137872 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Blocking and diluting buffer: 5% NFDM/TBST

    Exposure time: 3 minutes

    All lanes: Immunoprecipitation - Anti-LEF1 antibody [EPR2029Y] (AB137872)

    Predicted band size: 44 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 antibody [EPR2029Y] (ab137872), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 antibody [EPR2029Y] (ab137872)

    ab137872 staining LEF1 in paraffin embedded mouse spleen tissue by Immunohistochemistry. Antigen retrieval was performed by heat mediation using ab93684 (Tris/EDTA buffer, ph 9). Samples were incubated with primary antibody at 1/2000 dilution. A ready to use Goat Anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on T cells in periarterial lymphatic sheath of mouse spleen is observed (PMID: 21685909).

  • Western blot - Anti-LEF1 antibody [EPR2029Y] (ab137872), expandable thumbnail

    Western blot - Anti-LEF1 antibody [EPR2029Y] (ab137872)

    Blocking/Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-LEF1 antibody [EPR2029Y] (AB137872) at 1/2000 dilution

    All lanes: Rat thymus tissue lysate at 20 µg

    Secondary

    All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 44 kDa

  • Western blot - Anti-LEF1 antibody [EPR2029Y] (ab137872), expandable thumbnail

    Western blot - Anti-LEF1 antibody [EPR2029Y] (ab137872)

    Blocking/Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-LEF1 antibody [EPR2029Y] (AB137872) at 1/10000 dilution

    All lanes: Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate at 10 µg

    Secondary

    All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 44 kDa

  • Western blot - Anti-LEF1 antibody [EPR2029Y] (ab137872), expandable thumbnail

    Western blot - Anti-LEF1 antibody [EPR2029Y] (ab137872)

    Blocking and diluting buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-LEF1 antibody [EPR2029Y] (AB137872) at 1/1000 dilution

    All lanes: His-tagged mouse LEF-1 recombinant protein (aa1-397) at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution

    Predicted band size: 44 kDa

    Observed band size: 44 kDa

    Exposure time: 1s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 antibody [EPR2029Y] (ab137872), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 antibody [EPR2029Y] (ab137872)

    Tissue Microarrays stained for Anti-LEF1 antibody [EPR2029Y] using ab137872 in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negaive (cross mark) staining per sample type tested. The sections were incubated with ab137872 for 30 mins at room temperature used at 1:2000 dilution (1.05 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody (ab209101). Counterstain was Hematoxylin.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 antibody [EPR2029Y] (ab137872), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 antibody [EPR2029Y] (ab137872)

    ab137872 staining LEF1 in paraffin embedded human melanoma tissue by Immunohistochemistry. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.  Samples were incubated with primary antibody at 1/2000 dilution for 30 mins at room temperature. A ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 antibody [EPR2029Y] (ab137872), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 antibody [EPR2029Y] (ab137872)

    ab137872 staining LEF1 in paraffin embedded human thymona tissue by Immunohistochemistry. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.  Samples were incubated with primary antibody at 1/2000 dilution for 30 mins at room temperature. A ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 antibody [EPR2029Y] (ab137872), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 antibody [EPR2029Y] (ab137872)

    Immunohistochemical analysis of formalin fixed paraffin embedded human tonsil labelling LEF1 with ab137872 at a concentration of 0.5 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32 mins at 100°C with ULTRA cell conditioning solution (CC1, pH 8.5). ab137872 anti LEF1 antibody [EPR2029Y] was incubated at 37°C for 16 mins. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

  • Flow Cytometry (Intracellular) - Anti-LEF1 antibody [EPR2029Y] (ab137872), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-LEF1 antibody [EPR2029Y] (ab137872)

    Flow cytometry overlay histogram showing left Jurkat positive cells and right negative HeLa stained with ab137872 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab137872) (1x 106 in 100μl at 0.2μg/ml (1/11500)) for 30min at 22°C.

    The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min at 22°C

    Isotype control antibody (black line) was Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

    This antibody gave a positive signal in Jurkat Fixed with 4% formaldehyde (10 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 antibody [EPR2029Y] (ab137872), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 antibody [EPR2029Y] (ab137872)

    ab137872 staining LEF1 in paraffin embedded human melanoma tissue by Immunohistochemistry. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.  Samples were incubated with primary antibody at 1/2000 dilution for 30 mins at room temperature. A ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com