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AB222886

Anti-Lefty antibody

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(1 Publication)

Rabbit Polyclonal Lefty antibody. Suitable for WB and reacts with Rat, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human LEFTY1 aa 200-300.

View Alternative Names

LEFTB, LEFTYB, UNQ278/PRO317, LEFTY1, Left-right determination factor 1, Left-right determination factor B, Protein lefty-1, Protein lefty-B

1 Images
Western blot - Anti-Lefty antibody (AB222886)
  • WB

Supplier Data

Western blot - Anti-Lefty antibody (AB222886)

All lanes:

Western blot - Anti-Lefty antibody (ab222886) at 1/500 dilution

Lane 1:

Rat spleen lysate

Lane 2:

Mouse heart lysate

Lane 3:

Mouse liver lysate

Lane 4:

Mouse brain lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 41 kDa

Observed band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human LEFTY1 aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

O75610

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Alternative Products

Primary Antibodies

AB34593

Anti-LEFTY2 antibody [7C5G1H6H10]

Western blot - Anti-LEFTY2 antibody [7C5G1H6H10] (AB34593)

  • 0
  • 0
Primary Antibodies

AB22569

Anti-Lefty antibody

Immunocytochemistry/ Immunofluorescence - Anti-Lefty antibody (AB22569)

  • 5
  • 2

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

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Primary Antibodies

AB81287

Anti-Nodal antibody [EP2058Y]

RabMAb|Recombinant

Western blot - Anti-Nodal antibody [EP2058Y] (AB81287)

  • 0
  • 0
Primary Antibodies

AB224637

Anti-SOX17 antibody [EPR20684]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-SOX17 antibody [EPR20684] (AB224637)

  • 4
  • 6
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lefty also known as Left-right determination factor is a protein that plays an essential role in the regulation of embryonic development. It comes in two forms: Lefty1 and Lefty2 both of which belong to the TGF-beta superfamily. The molecular mass of Lefty proteins is approximately 38 kDa. These proteins are expressed in various tissues including the heart nervous system and mesoderm during embryogenesis.
Biological function summary

Lefty proteins inhibit specific signaling pathways to control cell fate and patterning during development. They act as antagonists to Activin and Nodal signaling critical components in embryonic patterning. Lefty does not form part of a complex but interacts closely with receptors that mediate the signaling pathways it inhibits. By modulating these pathways Lefty has an important function in ensuring symmetric organ positioning.

Pathways

Lefty proteins are involved in the Nodal signaling pathway which is essential for determining the left-right asymmetry in developing embryos. In this pathway Lefty acts against Nodal activity maintaining a balance that is critical for proper organ placement. Other proteins related to this pathway include Smad2 and Smad3 which transduce Nodal signals and are themselves modulated through Lefty activity.

Disruptions in Lefty signaling have been associated with abnormal heart and organ development such as situs inversus and heterotaxy syndrome where organs are misplaced or incorrectly oriented. Lefty interacts with Nodal and Activin proteins and anomalies in these interactions can lead to these developmental conditions. Understanding Lefty's role offers potential therapeutic strategies for correcting such congenital disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for left-right axis determination as a regulator of LEFTY2 and NODAL.
See full target information LEFTY1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 64:15 PubMed38095908

2023

JAM-C Is Important for Lens Epithelial Cell Proliferation and Lens Fiber Maturation in Murine Lens Development.

Applications

Unspecified application

Species

Unspecified reactive species

Qihang Sun,Jiani Li,Jingyu Ma,Yuxing Zheng,Rong Ju,Xuri Li,Xiangrong Ren,Lijuan Huang,Rongyuan Chen,Xuhua Tan,Lixia Luo
View all publications
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Product promise

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