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AB115224

Anti-LEFTY2 antibody [7C5G1H6H10]

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(1 Publication)

Mouse Monoclonal LEFTY2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human LEFTY2.

View Alternative Names

EBAF, LEFTA, LEFTYA, TGFB4, PSEC0024, LEFTY2, Left-right determination factor 2, Endometrial bleeding-associated factor, Left-right determination factor A, Protein lefty-2, Protein lefty-A, Transforming growth factor beta-4, TGF-beta-4

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEFTY2 antibody [7C5G1H6H10] (AB115224)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEFTY2 antibody [7C5G1H6H10] (AB115224)

Human uterus, myometrium (formalin fixed, paraffin embedded) stained with ab115224 at 10 ug/ml followed by biotinylated anti Mouse IgG secondary antibody, alkaline phosphatase streptavidin and chromogen.

Western blot - Anti-LEFTY2 antibody [7C5G1H6H10] (AB115224)
  • WB

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Western blot - Anti-LEFTY2 antibody [7C5G1H6H10] (AB115224)

All lanes:

Western blot - Anti-LEFTY2 antibody [7C5G1H6H10] (ab115224) at 1/2000 dilution

All lanes:

partially purified recombinant 6X His tagged human LEFTY2 at 1000 µg

Predicted band size: 41 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7C5G1H6H10

Isotype

IgG2a

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human LEFTY2.

O00292

Specificity

May cross react with the highly homologous LEFTY1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Tripotassium orthophosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LEFTY2 also known as Left-Right Determination Factor 2 is a member of the transforming growth factor beta (TGF-β) superfamily. This protein has a molecular mass of approximately 38 kDa. LEFTY2 is expressed mainly in visceral endoderm and left side mesoderm tissues. The gene encoding LEFTY2 is found within the human chromosome 1. This protein plays a mechanical role in the regulation of cellular signaling and developmental processes.
Biological function summary

LEFTY2 functions as an important regulator in ensuring proper left-right asymmetry during embryonic development. It is a monomeric protein that is not part of a larger complex. LEFTY2 contributes to the modulation of Nodal signaling inhibiting excessive activity that can disrupt normal development. This modulation ensures the establishment of asymmetric organ positioning and plays an important role in maintaining developmental balance in the body.

Pathways

LEFTY2 integrates into the Nodal signaling pathway and the Activin signaling pathway. The interaction with Nodal proteins narrows down Nodal signaling ensuring the accurate development of the left-right axis. LEFTY2 also relates functionally and antagonistically to other proteins like Nodal and Activin A balancing their signaling actions to regulate developmental processes.

LEFTY2 has a connection with asymmetry-related conditions such as situs inversus where organ placement within the body is mirrored from normal positions. Additionally research links irregular LEFTY2 expression to some cancer types including ovarian cancer. Through these disorders LEFTY2 interacts with proteins like Nodal which plays a role in cancer cell proliferation by influencing the signaling pathways that govern cellular growth and differentiation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for left-right (L-R) asymmetry determination of organ systems in mammals. May play a role in endometrial bleeding.
See full target information LEFTY2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 10:5492 PubMed31792210

2019

Genome-wide CRISPR screen identifies ELP5 as a determinant of gemcitabine sensitivity in gallbladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sunwang Xu,Ming Zhan,Cen Jiang,Min He,Linhua Yang,Hui Shen,Shuai Huang,Xince Huang,Ruirong Lin,Yongheng Shi,Qiang Liu,Wei Chen,Man Mohan,Jian Wang
View all publications
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Product promise

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