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AB34593

Anti-LEFTY2 antibody [7C5G1H6H10]

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(1 Publication)

Mouse Monoclonal LEFTY2 antibody. Suitable for WB and reacts with Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Protein within Human LEFTY2.

View Alternative Names

EBAF, LEFTA, LEFTYA, TGFB4, PSEC0024, LEFTY2, Left-right determination factor 2, Endometrial bleeding-associated factor, Left-right determination factor A, Protein lefty-2, Protein lefty-A, Transforming growth factor beta-4, TGF-beta-4

1 Images
Western blot - Anti-LEFTY2 antibody [7C5G1H6H10] (AB34593)
  • WB

Unknown

Western blot - Anti-LEFTY2 antibody [7C5G1H6H10] (AB34593)

All lanes:

Western blot - Anti-LEFTY2 antibody [7C5G1H6H10] (ab34593) at 1/2000 dilution

Lane 1:

1µg 6X His tagged unrelated protein (shows that the antibody does not recognize the epitope tag)

Lane 2:

1µg partially purified recombinant 6X his tagged human Lefty

Secondary

All lanes:

IRDye<sup>TM</sup>800 Goat anti-mouse IgG at 1/1000 dilution

Predicted band size: 41 kDa

Observed band size: 30 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7C5G1H6H10

Isotype

IgG2a

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Protein within Human LEFTY2. The exact immunogen used to generate this antibody is proprietary information.

O00292

Specificity

This protein A purified mouse monoclonal antibody reacts with a 30 kDa protein corresponding to human LEFTY. No reactivity occurs against 6X HIS tag. Significant sequence homology exists between the human and mouse forms of LEFTY. Cross reactivity is expected with mouse and rat forms of the protein. Reactivity with other sources has not been determined.

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Alternative Products

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Complementary Products

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Human LEFTY2 ELISA Kit

Sandwich ELISA - Human LEFTY2 ELISA Kit (AB313982)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>" } } }
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Product details

Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse Sp2/0 myeloma cells.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
This protein A purified mouse monoclonal antibody reacts with a 30 kDa protein corresponding to human LEFTY.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LEFTY2 also known as Left-Right Determination Factor 2 is a member of the transforming growth factor beta (TGF-β) superfamily. This protein has a molecular mass of approximately 38 kDa. LEFTY2 is expressed mainly in visceral endoderm and left side mesoderm tissues. The gene encoding LEFTY2 is found within the human chromosome 1. This protein plays a mechanical role in the regulation of cellular signaling and developmental processes.
Biological function summary

LEFTY2 functions as an important regulator in ensuring proper left-right asymmetry during embryonic development. It is a monomeric protein that is not part of a larger complex. LEFTY2 contributes to the modulation of Nodal signaling inhibiting excessive activity that can disrupt normal development. This modulation ensures the establishment of asymmetric organ positioning and plays an important role in maintaining developmental balance in the body.

Pathways

LEFTY2 integrates into the Nodal signaling pathway and the Activin signaling pathway. The interaction with Nodal proteins narrows down Nodal signaling ensuring the accurate development of the left-right axis. LEFTY2 also relates functionally and antagonistically to other proteins like Nodal and Activin A balancing their signaling actions to regulate developmental processes.

LEFTY2 has a connection with asymmetry-related conditions such as situs inversus where organ placement within the body is mirrored from normal positions. Additionally research links irregular LEFTY2 expression to some cancer types including ovarian cancer. Through these disorders LEFTY2 interacts with proteins like Nodal which plays a role in cancer cell proliferation by influencing the signaling pathways that govern cellular growth and differentiation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for left-right (L-R) asymmetry determination of organ systems in mammals. May play a role in endometrial bleeding.
See full target information LEFTY2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular and infection microbiology 10:592906 PubMed33569353

2021

Factor H Is Bound by Outer Membrane-Displayed Carbohydrate Metabolism Enzymes of Extraintestinal Pathogenic and Contributes to Opsonophagocytosis Resistance in Bacteria.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Sun,Bin Xu,Xiangkai Zhuge,Fang Tang,Xuhang Wang,Qianwen Gong,Rui Chen,Feng Xue,Jianjun Dai
View all publications
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Product promise

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