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AB229668

Anti-LEFTY2 antibody

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(3 Publications)

Rabbit Polyclonal LEFTY2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LEFTY2 aa 50 to C-terminus.

View Alternative Names

EBAF, LEFTA, LEFTYA, TGFB4, PSEC0024, LEFTY2, Left-right determination factor 2, Endometrial bleeding-associated factor, Left-right determination factor A, Protein lefty-2, Protein lefty-A, Transforming growth factor beta-4, TGF-beta-4

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEFTY2 antibody (AB229668)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEFTY2 antibody (AB229668)

Paraffin-embedded human colon cancer tissue stained for LEFTY2 using ab229668 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEFTY2 antibody (AB229668)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEFTY2 antibody (AB229668)

Paraffin-embedded human endometrial cancer tissue stained for LEFTY2 using ab229668 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-LEFTY2 antibody (AB229668)
  • WB

Supplier Data

Western blot - Anti-LEFTY2 antibody (AB229668)

All lanes:

Western blot - Anti-LEFTY2 antibody (ab229668) at 1/1000 dilution

All lanes:

A431 (human epidermoid carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat anti-rabbit IgG at 1/10000 dilution

Predicted band size: 41 kDa

Observed band size: 41 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human LEFTY2 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O00292

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Alternative Products

Primary Antibodies

AB115224

Anti-LEFTY2 antibody [7C5G1H6H10]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEFTY2 antibody [7C5G1H6H10] (AB115224)

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Complementary Products

Assay Kits

AB313982

Human LEFTY2 ELISA Kit

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Secondary Antibodies

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Primary Antibodies

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Flow Cytometry - Anti-Human IgM antibody [EPR20731] (AB212201)

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Primary Antibodies

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Primary Antibodies

AB275931

Anti-C5 / C5b antibody [EPR23940-3]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5 / C5b antibody [EPR23940-3] (AB275931)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LEFTY2 also known as Left-Right Determination Factor 2 is a member of the transforming growth factor beta (TGF-β) superfamily. This protein has a molecular mass of approximately 38 kDa. LEFTY2 is expressed mainly in visceral endoderm and left side mesoderm tissues. The gene encoding LEFTY2 is found within the human chromosome 1. This protein plays a mechanical role in the regulation of cellular signaling and developmental processes.
Biological function summary

LEFTY2 functions as an important regulator in ensuring proper left-right asymmetry during embryonic development. It is a monomeric protein that is not part of a larger complex. LEFTY2 contributes to the modulation of Nodal signaling inhibiting excessive activity that can disrupt normal development. This modulation ensures the establishment of asymmetric organ positioning and plays an important role in maintaining developmental balance in the body.

Pathways

LEFTY2 integrates into the Nodal signaling pathway and the Activin signaling pathway. The interaction with Nodal proteins narrows down Nodal signaling ensuring the accurate development of the left-right axis. LEFTY2 also relates functionally and antagonistically to other proteins like Nodal and Activin A balancing their signaling actions to regulate developmental processes.

LEFTY2 has a connection with asymmetry-related conditions such as situs inversus where organ placement within the body is mirrored from normal positions. Additionally research links irregular LEFTY2 expression to some cancer types including ovarian cancer. Through these disorders LEFTY2 interacts with proteins like Nodal which plays a role in cancer cell proliferation by influencing the signaling pathways that govern cellular growth and differentiation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for left-right (L-R) asymmetry determination of organ systems in mammals. May play a role in endometrial bleeding.
See full target information LEFTY2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of dental sciences 20:1681-1688 PubMed40654474

2025

Silencing miR-1291-LEFTY2 axis diminishes the myofibroblast activities and reactive oxygen species generation of fibrotic buccal mucosal fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Yung Chou,Shih-Min Wang,Pei-Ling Hsieh,Yi-Wen Liao,Cheng-Chia Yu,Chang-Yi Tsai

International journal of molecular sciences 26: PubMed40244244

2025

The Therapeutic Potential of ADSC-Secreted LEFTY2 in Treating Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Wu Li,Hsueh-Hui Yang,Tzyy-Wen Chiou,Peng-Yeong Woon,Yue-Xuan Xu,Cynthia Tjandra,Ivan Wijaya,Horng-Jyh Harn,Shinn-Zong Lin

International journal of molecular medicine 42:1229-1236 PubMed29845221

2018

miR‑215 promotes epithelial to mesenchymal transition and proliferation by regulating LEFTY2 in endometrial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxu Gao,Yan Cai,Ruifang An
View all publications
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Product promise

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