AB244326

Anti-LEPRE1/P3H1 antibody

Name: Anti-LEPRE1/P3H1 antibody (ab244326) | Abcam
Brand: Abcam Limited
SKU: AB244326
Price: 630 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(2 Publications)

Rabbit Polyclonal LEPRE1/P3H1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human P3H1 aa 300-450.

View Alternative Names

GROS1, LEPRE1, PSEC0109, P3H1, Prolyl 3-hydroxylase 1, Growth suppressor 1, Leucine- and proline-enriched proteoglycan 1, Leprecan-1

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEPRE1/P3H1 antibody (AB244326)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEPRE1/P3H1 antibody (AB244326)

Formalin-fixed, paraffin-embedded human liver tissue stained for LEPRE1/P3H1 with ab244326 at a 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEPRE1/P3H1 antibody (AB244326)

Formalin-fixed, paraffin-embedded human prostate tissue stained for LEPRE1/P3H1 with ab244326 at a 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEPRE1/P3H1 antibody (AB244326)

Formalin-fixed, paraffin-embedded human cervix tissue stained for LEPRE1/P3H1 with ab244326 at a 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEPRE1/P3H1 antibody (AB244326)

Formalin-fixed, paraffin-embedded human placenta tissue stained for LEPRE1/P3H1 with ab244326 at a 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEPRE1/P3H1 antibody (AB244326)

Formalin-fixed, paraffin-embedded human pancreas tissue shows absence of immunoreactivity for LEPRE1/P3H1 when incubated with ab244326 at a 1/50 dilution (negative control).

Unknown

Western blot - Anti-LEPRE1/P3H1 antibody (AB244326)

All lanes:

Western blot - Anti-LEPRE1/P3H1 antibody (ab244326) at 0.4 µg/mL

Lane 1:

U-2 OS (Human bone osteosarcoma epithelial cell line) cell lysate

Lane 2:

MCF7 (Human breast adenocarcinoma cell line) cell lysate

Predicted band size: 83 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human P3H1 aa 300-450. The exact immunogen used to generate this antibody is proprietary information.

Q32P28

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LEPRE1 also known as P3H1 or prolyl 3-hydroxylase 1 is an enzyme involved in collagen synthesis with a molecular mass of approximately 71 kDa. It is a member of the prolyl hydroxylase family and carries out 3-hydroxylation of proline residues in collagen. The enzyme localizes largely to the endoplasmic reticulum where it contributes to the post-translational modification of collagen. LEPRE1 is ubiquitously expressed with higher concentrations observed in tissues rich in collagen such as skin bone and cartilage.

Biological function summary

LEPRE1 plays a fundamental role in the maturation of collagen which is an important structural protein in connective tissues. This enzyme functions as a part of the collagen prolyl 3-hydroxylation complex which includes cartilage-associated protein (CRTAP) and cyclophilin B (PPIB). The complex is responsible for the correct folding and stability of collagen molecules ensuring the proper formation of the extracellular matrix. This structural role is essential for maintaining tissue integrity and function.

Pathways

LEPRE1 is important in the collagen biosynthesis and modifying pathways. It interacts with other biosynthesis enzymes like lysyl oxidase and procollagen-proline dioxygenase. These proteins coordinate to execute post-translational modifications which stabilize the collagen triple helix structure. Pirh2 and Hsp47 act along with LEPRE1 in similar pathways helping in folding and secretion of collagen.

LEPRE1 mutations have been linked to connective tissue disorders most notably osteogenesis imperfecta a genetic disorder causing brittle bones. Such mutations impair collagen modification leading to defective collagen formation and bone fragility. Additionally dysregulation of LEPRE1 activity appears connected to disorder Ehlers-Danlos syndrome noted for joint hypermobility and skin elasticity issues. COL1A1 and COL1A2 two primary components of type I collagen often associate with LEPRE1 in disease contexts as mutations in these collagens lead to similar clinical manifestations.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Basement membrane-associated chondroitin sulfate proteoglycan (CSPG). Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens, especially types IV and V. May be involved in the secretory pathway of cells. Has growth suppressive activity in fibroblasts.

See full target information P3H1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Pharmacogenomics and personalized medicine 16:1041-1053 PubMed38058295

2023

Identification of P3H1 as a Predictive Prognostic Biomarker for Bladder Urothelial Carcinoma Based on the Cancer Genome Atlas Database.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanfeng Zhang,Yang Chen,Zhiming Chen,Xinye Zhou,Shaochuan Chen,Kaijian Lan,Zhiping Wang,Yonghai Zhang

Journal of integrative neuroscience 20:595-603 PubMed34645092

2021

The impact of CRMP4 SUMOylation on the Cav1.2 interaction, neurite outgrowth and thermal pain sensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

Shangdao Lai,Meiyuan Pan,Hongxing Liao,Jiayu Chen,Yuqing Jiang,Yalan Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LEPRE1/P3H1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEPRE1/P3H1 antibody (AB244326)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEPRE1/P3H1 antibody (AB244326)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEPRE1/P3H1 antibody (AB244326)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEPRE1/P3H1 antibody (AB244326)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEPRE1/P3H1 antibody (AB244326)

Western blot - Anti-LEPRE1/P3H1 antibody (AB244326)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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