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AB154799

Anti-LEPRE1/P3H1 antibody [EPR10193(B)]

5

(1 Review)

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(2 Publications)

Rabbit Recombinant Monoclonal LEPRE1/P3H1 antibody. Suitable for WB, I-ELISA, ICC/IF, Flow Cyt (Intra) and reacts with Human, Synthetic peptide - Human samples. Cited in 2 publications.

View Alternative Names

GROS1, LEPRE1, PSEC0109, P3H1, Prolyl 3-hydroxylase 1, Growth suppressor 1, Leucine- and proline-enriched proteoglycan 1, Leprecan-1

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (AB154799)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (AB154799)

Immunofluorescence analysis of HepG2 cells labeling LEPRE1/P3H1 with ab154799 at 1/250 dilution.

Flow Cytometry (Intracellular) - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (AB154799)
  • Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (AB154799)

Intracellular flow cytometric analysis of permeabilized A549 cells labeling LEPRE1/P3H1 with ab154799 at 1/10 dilution (red) or a negative rabbit IgG (green).

Immunocytochemistry/ Immunofluorescence - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (AB154799)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (AB154799)

Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling LEPRE1/P3H1 with Purified ab154799 at 1/500 dilution (5μg/ml). Cells were fixed with 100% methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

Indirect ELISA - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (AB154799)
  • I-ELISA

Unknown

Indirect ELISA - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (AB154799)

ELISA using ab154799 at varying antibody concentrations and antigen concentration at 1000 ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

Western blot - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (AB154799)
  • WB

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Western blot - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (AB154799)

All lanes:

Western blot - Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

A549 cell lysate at 10 µg

Lane 4:

Human placenta tissue lysate at 10 µg

Predicted band size: 83 kDa

false

  • Carrier free

    Anti-LEPRE1/P3H1 antibody [EPR10193(B)] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-LEPRE1/P3H1 antibody [EPR10193(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10193(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

I-ELISA, ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Synthetic peptide - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LEPRE1 also known as P3H1 or prolyl 3-hydroxylase 1 is an enzyme involved in collagen synthesis with a molecular mass of approximately 71 kDa. It is a member of the prolyl hydroxylase family and carries out 3-hydroxylation of proline residues in collagen. The enzyme localizes largely to the endoplasmic reticulum where it contributes to the post-translational modification of collagen. LEPRE1 is ubiquitously expressed with higher concentrations observed in tissues rich in collagen such as skin bone and cartilage.
Biological function summary

LEPRE1 plays a fundamental role in the maturation of collagen which is an important structural protein in connective tissues. This enzyme functions as a part of the collagen prolyl 3-hydroxylation complex which includes cartilage-associated protein (CRTAP) and cyclophilin B (PPIB). The complex is responsible for the correct folding and stability of collagen molecules ensuring the proper formation of the extracellular matrix. This structural role is essential for maintaining tissue integrity and function.

Pathways

LEPRE1 is important in the collagen biosynthesis and modifying pathways. It interacts with other biosynthesis enzymes like lysyl oxidase and procollagen-proline dioxygenase. These proteins coordinate to execute post-translational modifications which stabilize the collagen triple helix structure. Pirh2 and Hsp47 act along with LEPRE1 in similar pathways helping in folding and secretion of collagen.

LEPRE1 mutations have been linked to connective tissue disorders most notably osteogenesis imperfecta a genetic disorder causing brittle bones. Such mutations impair collagen modification leading to defective collagen formation and bone fragility. Additionally dysregulation of LEPRE1 activity appears connected to disorder Ehlers-Danlos syndrome noted for joint hypermobility and skin elasticity issues. COL1A1 and COL1A2 two primary components of type I collagen often associate with LEPRE1 in disease contexts as mutations in these collagens lead to similar clinical manifestations.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Basement membrane-associated chondroitin sulfate proteoglycan (CSPG). Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens, especially types IV and V. May be involved in the secretory pathway of cells. Has growth suppressive activity in fibroblasts.
See full target information P3H1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 12:22569 PubMed36581661

2022

Transcriptome analysis of a dog model of congestive heart failure shows that collagen-related 2-oxoglutarate-dependent dioxygenases contribute to heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Takahiro Isono,Takehiro Matsumoto,Masafumi Suzaki,Shigehisa Kubota,Susumu Kageyama,Akihiro Kawauchi,Atuyuki Wada

Cellular and molecular life sciences : CMLS 76:3899-3914 PubMed30993352

2019

Characterization of PPIB interaction in the P3H1 ternary complex and implications for its pathological mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Jiawei Wu,Wenting Zhang,Li Xia,Lingling Feng,Zimei Shu,Jing Zhang,Wei Ye,Naiyan Zeng,Aiwu Zhou
View all publications
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