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AB222823

Anti-LEPREL1 antibody

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(1 Publication)

Rabbit Polyclonal LEPREL1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human P3H2 aa 300-550.

View Alternative Names

LEPREL1, MLAT4, P3H2, Prolyl 3-hydroxylase 2, Leprecan-like protein 1, Myxoid liposarcoma-associated protein 4

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEPREL1 antibody (AB222823)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEPREL1 antibody (AB222823)

Paraffin-embedded human lung tissue stained for LEPREL1 with ab222823 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEPREL1 antibody (AB222823)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEPREL1 antibody (AB222823)

Paraffin-embedded human liver tissue stained for LEPREL1 with ab222823 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-LEPREL1 antibody (AB222823)
  • WB

Supplier Data

Western blot - Anti-LEPREL1 antibody (AB222823)

All lanes:

Western blot - Anti-LEPREL1 antibody (ab222823) at 1/2000 dilution

Lane 1:

Mouse lung lysate

Lane 2:

Mouse kidney lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 62 kDa,81 kDa

Observed band size: 25 kDa,61 kDa,65 kDa,81 kDa,90 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human P3H2 aa 300-550. The exact immunogen used to generate this antibody is proprietary information.

Q8IVL5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LEPREL1 also known as Prolyl 3-hydroxylase 2 (P3H2) or leprecan-like 1 is an enzyme that plays an important role in the hydroxylation of proline residues in collagen a fundamental process in collagen maturation. LEPREL1 weighs about 76 kDa and is a part of a family of enzymes involved in post-translational modifications. LEPREL1 expression predominantly occurs in the eye specifically in the retina but it can also be found in other tissues such as the heart and skeletal muscle.
Biological function summary

LEPREL1 performs mandatory activities in collagen triple-helix formation. It serves as a component of the endoplasmic reticulum-resident enzyme complex collaborating with additional enzymes like Collagen Prolyl Hydroxylase. These complexes ensure the correct folding of collagen fibrils contributing significantly to the structural integrity and function of connective tissues. Collagen biosynthesis mediated by LEPREL1 is essential for maintaining tissue architecture and functionality across various organ systems.

Pathways

Collagen biosynthesis and modification represents an important pathway involving LEPREL1. The ER-resident enzymes such as PLOD1 and PLOD2 work alongside LEPREL1 emphasizing its positioning within collagen biosynthesis pathways. Proper collagen hydroxylation ensures the strength and elasticity of mature collagens highlighting its relevance in tissue development and repair.

LEPREL1 associates strongly with early-onset glaucoma and connective tissue disorders like Ehlers-Danlos syndrome. Mutations in LEPREL1 can lead to disruptions in collagen maturation causing weaker structural proteins and contributing to disease manifestation. Additionally research shows its involvement with MYOC (myocilin) in glaucoma pathogenesis further linking collagen processing defects with ocular health issues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Prolyl 3-hydroxylase that catalyzes the post-translational formation of 3-hydroxyproline on collagens (PubMed : 18487197). Contributes to proline 3-hydroxylation of collagen COL4A1 and COL1A1 in tendons, the eye sclera and in the eye lens capsule (By similarity). Has high activity with the type IV collagen COL4A1, and lower activity with COL1A1 (PubMed : 18487197). Catalyzes hydroxylation of the first Pro in Gly-Pro-Hyp sequences where Hyp is 4-hydroxyproline (PubMed : 18487197). Has no activity on substrates that lack 4-hydroxyproline in the third position (PubMed : 18487197).
See full target information P3H2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bioscience reports 39: PubMed30975733

2019

MicroRNA-153 promotes brain-derived neurotrophic factor and hippocampal neuron proliferation to alleviate autism symptoms through inhibition of JAK-STAT pathway by LEPR.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Hui You,Zhi-Qiang Qin,Huan-Li Zhang,Zhao-Hong Yuan,Xin Yu
View all publications
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Product promise

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