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Rabbit Polyclonal LEPREL1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human P3H2 aa 300-550.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEPREL1 antibody (AB222823), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEPREL1 antibody (AB222823), expandable thumbnail
  • Western blot - Anti-LEPREL1 antibody (AB222823), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Form
Liquid
Clonality
Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human P3H2 aa 300-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IVL5

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Expected
Tested
Mouse
Tested
Expected

Tested
Tested

Species
Mouse
Dilution info
1/2000 - 1/5000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/20 - 1/200
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Prolyl 3-hydroxylase that catalyzes the post-translational formation of 3-hydroxyproline on collagens (PubMed:18487197). Contributes to proline 3-hydroxylation of collagen COL4A1 and COL1A1 in tendons, the eye sclera and in the eye lens capsule (By similarity). Has high activity with the type IV collagen COL4A1, and lower activity with COL1A1 (PubMed:18487197). Catalyzes hydroxylation of the first Pro in Gly-Pro-Hyp sequences where Hyp is 4-hydroxyproline (PubMed:18487197). Has no activity on substrates that lack 4-hydroxyproline in the third position (PubMed:18487197).

Alternative names

Recommended products

Rabbit Polyclonal LEPREL1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human P3H2 aa 300-550.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • Recombinant Fragment Protein within Human P3H2 aa 300-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IVL5
Purification technique
Affinity purification Protein G
Concentration
Loading...
Purification notes

Purity >95%.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

LEPREL1 also known as Prolyl 3-hydroxylase 2 (P3H2) or leprecan-like 1 is an enzyme that plays an important role in the hydroxylation of proline residues in collagen a fundamental process in collagen maturation. LEPREL1 weighs about 76 kDa and is a part of a family of enzymes involved in post-translational modifications. LEPREL1 expression predominantly occurs in the eye specifically in the retina but it can also be found in other tissues such as the heart and skeletal muscle.

Biological function summary

LEPREL1 performs mandatory activities in collagen triple-helix formation. It serves as a component of the endoplasmic reticulum-resident enzyme complex collaborating with additional enzymes like Collagen Prolyl Hydroxylase. These complexes ensure the correct folding of collagen fibrils contributing significantly to the structural integrity and function of connective tissues. Collagen biosynthesis mediated by LEPREL1 is essential for maintaining tissue architecture and functionality across various organ systems.

Pathways

Collagen biosynthesis and modification represents an important pathway involving LEPREL1. The ER-resident enzymes such as PLOD1 and PLOD2 work alongside LEPREL1 emphasizing its positioning within collagen biosynthesis pathways. Proper collagen hydroxylation ensures the strength and elasticity of mature collagens highlighting its relevance in tissue development and repair.

Associated diseases and disorders

LEPREL1 associates strongly with early-onset glaucoma and connective tissue disorders like Ehlers-Danlos syndrome. Mutations in LEPREL1 can lead to disruptions in collagen maturation causing weaker structural proteins and contributing to disease manifestation. Additionally research shows its involvement with MYOC (myocilin) in glaucoma pathogenesis further linking collagen processing defects with ocular health issues.

Product promise

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3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEPREL1 antibody (ab222823), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEPREL1 antibody (ab222823)

    Paraffin-embedded human liver tissue stained for LEPREL1 with ab222823 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEPREL1 antibody (ab222823), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEPREL1 antibody (ab222823)

    Paraffin-embedded human lung tissue stained for LEPREL1 with ab222823 at 1/100 dilution in immunohistochemical analysis.

  • Western blot - Anti-LEPREL1 antibody (ab222823), expandable thumbnail

    Western blot - Anti-LEPREL1 antibody (ab222823)

    All lanes: Western blot - Anti-LEPREL1 antibody (ab222823) at 1/2000 dilution

    Lane 1: Mouse lung lysate

    Lane 2: Mouse kidney lysate

    Secondary

    All lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 62 kDa, 81 kDa

    Observed band size: 25 kDa, 61 kDa, 65 kDa, 81 kDa, 90 kDa

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Product protocols

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