AB16227

Anti-Leptin antibody

Name: Anti-Leptin antibody (ab16227) | Abcam
Brand: Abcam Limited
SKU: AB16227
Price: 450 USD
Availability: InStock
Rating: 3 (3 reviews)

(3 Reviews)

(41 Publications)

Anti-Leptin antibody (ab16227) is a rabbit polyclonal antibody detecting Leptin in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse,.

- Over 30 publications
- Trusted since 2004

View Alternative Names

OB, OBS, LEP, Leptin, Obese protein, Obesity factor

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-Leptin antibody (AB16227)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Leptin antibody (AB16227)

Immunocytochemistry/Immunofluorescent analysis of Leptin (green) showing staining in the secretion of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab16227 in 3% BSA-PBS at a dilution of 1 : 100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Leptin antibody (AB16227)

Immunocytochemistry/Immunofluorescent analysis of Leptin (green) showing staining in the secretion of HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab16227 in 3% BSA-PBS at a dilution of 1 : 100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leptin antibody (AB16227)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leptin antibody (AB16227)

ab16227 staining human placenta.
Left panel : with primary antibody. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Leptin antibody (AB16227)

Immunocytochemistry/Immunofluorescent analysis of Leptin (green) showing staining in the secretion of NIH-3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab16227 in 3% BSA-PBS at a dilution of 1 : 100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.

Unknown

Western blot - Anti-Leptin antibody (AB16227)

All lanes:

Western blot - Anti-Leptin antibody (ab16227) at 1/500 dilution

All lanes:

Western blot - Recombinant Human Leptin protein (<a href='/en-us/products/proteins-peptides/recombinant-human-leptin-protein-ab51273'>ab51273</a>) at 1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 19 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human LEP aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

P41159

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Leptin antibody (ab16227) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, samples.

Trusted by the scientific community
Anti-Leptin (ab16227) was first used in a scientific publication in 2004 and has been cited over 30 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Precipitation Ammonium Sulphate

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Leptin also known as the OB protein is a hormone with a molecular weight of approximately 16 kDa. Primarily it is produced by adipocytes in white adipose tissue and to a lesser extent in the placenta stomach and other tissues. This protein plays a significant role in energy homeostasis by conveying information to the central nervous system regarding fat storage. In scientific studies leptin is often measured using methods like the leptin ELISA kit or mouse leptin assay to monitor its levels and function.

Biological function summary

Leptin regulates food intake and energy expenditure by binding to its receptors in the hypothalamus. It is an important part of the feedback loop to manage body weight and appetite. Leptin is not part of a larger protein complex and acts directly on its target tissues. By signal transduction pathways it influences cellular processes such as metabolism and even impacts fertility and immune responses.

Pathways

Leptin is involved in the neuroendocrine signaling pathway and the MAPK/ERK pathway. In the neuroendocrine system leptin interacts with proteins like proopiomelanocortin (POMC) and neuropeptide Y (NPY) to manage appetite and energy use. Additionally it activates the JAK/STAT pathway when binding to its receptor which influences gene expression involved in metabolism and inflammation.

Leptin is linked to obesity and type 2 diabetes. Leptin deficiencies or resistance can lead to metabolic disorders as the hormone's signaling is disrupted affecting energy balance and storage. Its connection to adiponectin another hormone derived from fat tissue is critical in understanding these conditions; while leptin promotes inflammation adiponectin has anti-inflammatory effects. Exploring these proteins' roles and their balance offers insights into treatments for obesity-related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways (PubMed : 15899045, PubMed : 17344214, PubMed : 19688109). In the hypothalamus, acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic for endothelial cell and affects innate and adaptive immunity (By similarity) (PubMed : 11460888, PubMed : 19688109, PubMed : 24340098, PubMed : 25060689, PubMed : 8589726). In the arcuate nucleus of the hypothalamus, activates by depolarization POMC neurons inducing FOS and SOCS3 expression to release anorexigenic peptides and inhibits by hyperpolarization NPY neurons inducing SOCS3 with a consequent reduction on release of orexigenic peptides (By similarity). In addition to its known satiety inducing effect, has a modulatory role in nutrient absorption. In the intestine, reduces glucose absorption by enterocytes by activating PKC and leading to a sequential activation of p38, PI3K and ERK signaling pathways which exerts an inhibitory effect on glucose absorption (PubMed : 24340098). Acts as a growth factor on certain tissues, through the activation of different signaling pathways increases expression of genes involved in cell cycle regulation such as CCND1, via JAK2-STAT3 pathway, or VEGFA, via MAPK1/3 and PI3K-AKT1 pathways (By similarity) (PubMed : 17344214). May also play an apoptotic role via JAK2-STAT3 pathway and up-regulation of BIRC5 expression (PubMed : 18242580). Pro-angiogenic, has mitogenic activity on vascular endothelial cells and plays a role in matrix remodeling by regulating the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) (PubMed : 11460888). In innate immunity, modulates the activity and function of neutrophils by increasing chemotaxis and the secretion of oxygen radicals. Increases phagocytosis by macrophages and enhances secretion of pro-inflammatory mediators. Increases cytotoxic ability of NK cells (PubMed : 12504075). Plays a pro-inflammatory role, in synergy with IL1B, by inducing NOS2 wich promotes the production of IL6, IL8 and Prostaglandin E2, through a signaling pathway that involves JAK2, PI3K, MAP2K1/MEK1 and MAPK14/p38 (PubMed : 15899045, PubMed : 19688109). In adaptive immunity, promotes the switch of memory T-cells towards T helper-1 cell immune responses (By similarity). Increases CD4(+)CD25(-) T-cell proliferation and reduces autophagy during TCR (T-cell receptor) stimulation, through MTOR signaling pathway activation and BCL2 up-regulation (PubMed : 25060689).

See full target information LEP

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Publications (41)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 15:1579843 PubMed40661777

2025

Analysis of the molecular subtypes and prognostic models of anoikis-related genes in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Shen,Kang Hou,Jifeng Zhang,Xiaodong Li

Mikrochimica acta 192:63 PubMed39789177

2025

Quantitative determination of leptin hormone using gold nanoparticle-based lateral flow assay.

Applications

Unspecified application

Species

Unspecified reactive species

Erhan Zor,Sabri Alpaydin,Haluk Bingol

BMC molecular and cell biology 25:26 PubMed39695926

2024

LEP O-GlcNAcylation inactivates NF-κB pathway by suppressing LEP protein level and thus mediates cellular senescence and osteogenic differentiation in mouse mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuang Zhang,Chaoqing Zhou,Lili Yu

Cardiovascular diabetology 23:444 PubMed39696214

2024

Pericoronary adipose tissue radiomics features as imaging markers for coronary artery disease risk assessment: insights from gene expression analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Li,Weimin Zhang,Yahui Hu,Zheng Xu,Qiang Huo,Haicheng Qi,Qian Liu,Yan Xing

Endocrinology and metabolism (Seoul, Korea) 39:932-945 PubMed39566547

2024

Alterations in Adipose Tissue and Adipokines in Heterozygous APE1/Ref-1 Deficient Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Eun-Ok Lee,Hao Jin,Sungmin Kim,Hee Kyoung Joo,Yu Ran Lee,Soo Yeon An,Shuyu Piao,Kwon Ho Lee,Byeong Hwa Jeon

JCI insight 9: PubMed38716728

2024

Whole-body deletion of Endospanin 1 protects from obesity-associated deleterious metabolic alterations.

Applications

Unspecified application

Species

Unspecified reactive species

Arturo Roca-Rivada,Marcio Do Cruzeiro,Raphaël Gp Denis,Qiang Zhang,Christine Rouault,Yves Rouillé,Jean-Marie Launay,Céline Cruciani-Guglielmacci,Virginie Mattot,Karine Clément,Ralf Jockers,Julie Dam

Nature communications 15:3769 PubMed38704393

2024

Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrow.

Applications

Unspecified application

Species

Unspecified reactive species

Tongling Huang,Zhaocheng Lu,Zihui Wang,Lixin Cheng,Lu Gao,Jun Gao,Ning Zhang,Chang-An Geng,Xiaoli Zhao,Huaiyu Wang,Chi-Wai Wong,Kelvin W K Yeung,Haobo Pan,William Weijia Lu,Min Guan

The American journal of pathology 194:253-263 PubMed38029922

2023

Nonmetastatic Axillary Lymph Nodes Have Distinct Morphology and Immunophenotype in Obese Patients with Breast Cancer at Risk for Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Qingyuan Song,Kristen E Muller,Liesbeth M Hondelink,Roberta M diFlorio-Alexander,Margaret R Karagas,Saeed Hassanpour

Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer 31:506 PubMed37542585

2023

Acupuncture improves immunity and fatigue after chemotherapy in breast cancer patients by inhibiting the Leptin/AMPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jinxia Li,Ruiyang Fu,Xiaoqing Guo,Zhongqiang Pan,Jingjun Xie

Bone & joint research 12:486-493 PubMed37536684

2023

Adiponectin inhibits fibrosis of the palmar aponeurosis in Dupuytren's contracture in male patients.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshiaki Yamanaka,Takafumi Tajima,Yoshitaka Tsujimura,Toichiro Naito,Yosuke Mano,Manabu Tsukamoto,Yukichi Zenke,Akinori Sakai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Leptin antibody

Immunocytochemistry/ Immunofluorescence - Anti-Leptin antibody (AB16227)

Immunocytochemistry/ Immunofluorescence - Anti-Leptin antibody (AB16227)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leptin antibody (AB16227)

Immunocytochemistry/ Immunofluorescence - Anti-Leptin antibody (AB16227)

Western blot - Anti-Leptin antibody (AB16227)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (41)

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