AB104403

Anti-Leptin Receptor antibody

Name: Anti-Leptin Receptor antibody (ab104403) | Abcam
Brand: Abcam Limited
SKU: AB104403
Price: 410 USD
Availability: InStock
Rating: 3 (3 reviews)

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What is this?

(3 Reviews)

(16 Publications)

Rabbit Polyclonal Leptin Receptor antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 16 publications.

View Alternative Names

CD295, DB, OBR, LEPR, Leptin receptor, LEP-R, HuB219, OB receptor, OB-R

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leptin Receptor antibody (AB104403)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leptin Receptor antibody (AB104403)

IHC image of aLeptin Receptor staining in Human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab104403, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Unknown

Western blot - Anti-Leptin Receptor antibody (AB104403)

Leptin Receptor contains an exstensive number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

All lanes:

Western blot - Anti-Leptin Receptor antibody (ab104403) at 1 µg/mL

All lanes:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 132 kDa

Observed band size: 150 kDa,40 kDa,49 kDa

true

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "" }, "Macaque monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Leptin Receptor commonly known as LEPR or OB-R is a protein that plays an important role in energy homeostasis and weight regulation. Mechanically it functions as a receptor for leptin the hormone predominantly produced by adipocytes. Leptin binding triggers activation of intracellular signaling pathways. The molecular weight of the Leptin Receptor can vary as it exists in several isoforms due to alternative splicing. It is prominently expressed in the hypothalamus and other tissues involved in energy regulation such as the liver and skeletal muscle.

Biological function summary

The Leptin Receptor acts as a gateway for leptin to exert its effects on the central nervous system and peripheral tissues. When leptin binds to LEPR it facilitates the formation of receptor complexes that engage downstream signaling molecules like STAT3 and MAPK. This results in regulation of appetite and energy expenditure. LEPR is not part of a complex with other receptors but it interacts with intracellular signaling proteins to mediate leptin's physiological effects.

Pathways

The Leptin Receptor is integral to the JAK-STAT signaling pathway and the MAPK pathway both of which are important in maintaining energy balance. LEPR activates JAK2 upon ligand binding leading to phosphorylation events that propagate the signal. It is closely associated with other proteins like SOCS3 which acts as a feedback inhibitor of leptin signaling playing a balancing role in the pathways mentioned.

The Leptin Receptor has significant associations with obesity and certain types of diabetes. LEPR mutations or deficiencies can lead to hyperphagia leading to severe obesity due to the disruption of normal leptin signaling. Additionally interactions between leptin and insulin signaling pathways can impact the development of Type 2 diabetes. In these conditions the Leptin Receptor often interacts with the insulin receptor highlighting its role in the complex network of metabolic regulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for hormone LEP/leptin (Probable) (PubMed : 22405007). On ligand binding, mediates LEP central and peripheral effects through the activation of different signaling pathways such as JAK2/STAT3 and MAPK cascade/FOS. In the hypothalamus, LEP acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones (By similarity) (PubMed : 9537324). In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic and affects innate and adaptive immunity (PubMed : 12504075, PubMed : 25060689, PubMed : 8805376). Control of energy homeostasis and melanocortin production (stimulation of POMC and full repression of AgRP transcription) is mediated by STAT3 signaling, whereas distinct signals regulate NPY and the control of fertility, growth and glucose homeostasis. Involved in the regulation of counter-regulatory response to hypoglycemia by inhibiting neurons of the parabrachial nucleus. Has a specific effect on T lymphocyte responses, differentially regulating the proliferation of naive and memory T -ells. Leptin increases Th1 and suppresses Th2 cytokine production (By similarity).. Isoform A. May transport LEP across the blood-brain barrier. Binds LEP and mediates LEP endocytosis. Does not induce phosphorylation of and activate STAT3.. Isoform E. Antagonizes Isoform A and isoform B-mediated LEP binding and endocytosis.

See full target information LEPR

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Biomimetics (Basel, Switzerland) 9: PubMed39590245

2024

A Murine Model of Non-Wear-Particle-Induced Aseptic Loosening.

Applications

Unspecified application

Species

Unspecified reactive species

Vincentius Suhardi,Anastasia Oktarina,Yingzhen Niu,Branden Sosa,Julia Retzky,Matthew Greenblatt,Lionel Ivashkiv,Mathias Bostrom,Xu Yang

Nature biomedical engineering 8:1285-1307 PubMed39085645

2024

Prevention and treatment of peri-implant fibrosis by functionally inhibiting skeletal cells expressing the leptin receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Vincentius Jeremy Suhardi,Anastasia Oktarina,Mohammed Hammad,Yingzhen Niu,Qingdian Li,Andrew Thomson,Juan Lopez,Jason McCormick,Ugur M Ayturk,Matthew B Greenblatt,Lionel B Ivashkiv,Mathias P G Bostrom,Xu Yang

JBMR plus 6:e10596 PubMed35309866

2022

A Subset of Osteosarcoma Bears Markers of CXCL12-Abundant Reticular Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Branden R Sosa,Ziqi Wang,John H Healey,Meera Hameed,Matthew B Greenblatt

Frontiers in veterinary science 8:625147 PubMed33644151

2021

Serum and Tissue Expression Levels of Leptin and Leptin Receptor Are Putative Markers of Specific Feline Mammary Carcinoma Subtypes.

Applications

Unspecified application

Species

Unspecified reactive species

Andreia Gameiro,Catarina Nascimento,Ana Catarina Urbano,Jorge Correia,Fernando Ferreira

Evidence-based complementary and alternative medic 2020:2532081 PubMed33014100

2020

Effect of Qing'e Decoction on Leptin/Leptin Receptor and Bone Metabolism in Naturally Aging Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Pan Sun,Yuanyuan Zhang,Zhenpu Wei,Zhiqiang Wang,Shiming Guo,Yanping Lin

Journal of orthopaedic translation 21:91-99 PubMed32110508

2020

Leptin receptor-expressing cells represent a distinct subpopulation of notochord-derived cells and are essential for disc homoeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Gao,Jinhua Yin,Xiaolong Xu,Jing Fan,Di Wang,Chao Zheng,Weiguang Lu,Pengzhen Cheng,Jicheng Sun,Dong Wang,Lu Li,Bo O Zhou,Liu Yang,Zhuojing Luo

PloS one 14:e0226010 PubMed31794592

2019

Influence of pre-pregnancy body mass index (p-BMI) and gestational weight gain (GWG) on DNA methylation and protein expression of obesogenic genes in umbilical vein.

Applications

Unspecified application

Species

Unspecified reactive species

Erika Chavira-Suárez,Angélica Jazmín Ramírez-Mendieta,Sofía Martínez-Gutiérrez,Paola Zárate-Segura,Jorge Beltrán-Montoya,Nidia Carolina Espinosa-Maldonado,Juan Carlos de la Cerda-Ángeles,Felipe Vadillo-Ortega

PeerJ 7:e7624 PubMed31592340

2019

Expression of leptin and leptin receptors in colorectal cancer-an immunohistochemical study.

Applications

Unspecified application

Species

Unspecified reactive species

Saad M Al-Shibli,Norra Harun,Abdelkader E Ashour,Mohd Hanif B Mohd Kasmuri,Shaikh Mizan

Medical science monitor : international medical jo 25:3005-3013 PubMed31015393

2019

The Effect of the Leptin and Leptin Receptor Expression on the Efficacy of Neoadjuvant Chemotherapy in Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Kong,Qian Dong,Hong Ji,Meixiang Sang,Yan Ding,Meng Zhao,Huichai Yang,Cuizhi Geng

Clinical epigenetics 11:20 PubMed30732639

2019

Maternal obesity influences expression and DNA methylation of the adiponectin and leptin systems in human third-trimester placenta.

Applications

Unspecified application

Species

Unspecified reactive species

Perrine Nogues,Esther Dos Santos,Hélène Jammes,Paul Berveiller,Lucie Arnould,François Vialard,Marie-Noëlle Dieudonné

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Leptin Receptor antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leptin Receptor antibody (AB104403)

Western blot - Anti-Leptin Receptor antibody (AB104403)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (16)

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