Goat Polyclonal Leptin Receptor antibody. Suitable for WB, IHC-P, Flow Cyt and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human LEPR aa 800-850.
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Publications
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- Animals : an open access journal from MDPI 10:2020Leptin System in Obese Dog Skin: A Pilot Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMargherita Maranesi,Antonio Di Loria,Cecilia Dall'Aglio,Diego Piantedosi,Elvio Lepri,Paolo Ciaramella,Francesca MercatiPubMed 33316917
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human LEPR aa 800-850. The exact immunogen used to generate this antibody is proprietary information. Database link P48357
Reactivity data
Select an application| WB | IHC-P | Flow Cyt | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Tested | Predicted | Expected |
| Rat | Tested | Predicted | Expected |
| Dog | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
Species Mouse | Dilution info 1.00000-2.00000 µg/mL | Notes - |
Species Rat | Dilution info 1.00000-2.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Receptor for hormone LEP/leptin (Probable) (PubMed:22405007). On ligand binding, mediates LEP central and peripheral effects through the activation of different signaling pathways such as JAK2/STAT3 and MAPK cascade/FOS. In the hypothalamus, LEP acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones (By similarity) (PubMed:9537324). In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic and affects innate and adaptive immunity (PubMed:12504075, PubMed:25060689, PubMed:8805376). Control of energy homeostasis and melanocortin production (stimulation of POMC and full repression of AgRP transcription) is mediated by STAT3 signaling, whereas distinct signals regulate NPY and the control of fertility, growth and glucose homeostasis. Involved in the regulation of counter-regulatory response to hypoglycemia by inhibiting neurons of the parabrachial nucleus. Has a specific effect on T lymphocyte responses, differentially regulating the proliferation of naive and memory T -ells. Leptin increases Th1 and suppresses Th2 cytokine production (By similarity). Isoform A. May transport LEP across the blood-brain barrier. Binds LEP and mediates LEP endocytosis. Does not induce phosphorylation of and activate STAT3. Isoform E. Antagonizes Isoform A and isoform B-mediated LEP binding and endocytosis.
Alternative names
CD295, DB, OBR, LEPR, Leptin receptor, LEP-R, HuB219, OB receptor, OB-R
Recommended products
Goat Polyclonal Leptin Receptor antibody. Suitable for WB, IHC-P, Flow Cyt and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human LEPR aa 800-850.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human LEPR aa 800-850. The exact immunogen used to generate this antibody is proprietary information. Database link P48357
- Purification technique
- Affinity purification Immunogen
- Specificity
This antibody is expected to recognise all three reported isoforms (NP_002294.2; NP_001003679.1; NP_001003680.1) of Leptin Receptor.
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Leptin Receptor commonly known as LEPR or OB-R is a protein that plays an important role in energy homeostasis and weight regulation. Mechanically it functions as a receptor for leptin the hormone predominantly produced by adipocytes. Leptin binding triggers activation of intracellular signaling pathways. The molecular weight of the Leptin Receptor can vary as it exists in several isoforms due to alternative splicing. It is prominently expressed in the hypothalamus and other tissues involved in energy regulation such as the liver and skeletal muscle.
- Biological function summary
The Leptin Receptor acts as a gateway for leptin to exert its effects on the central nervous system and peripheral tissues. When leptin binds to LEPR it facilitates the formation of receptor complexes that engage downstream signaling molecules like STAT3 and MAPK. This results in regulation of appetite and energy expenditure. LEPR is not part of a complex with other receptors but it interacts with intracellular signaling proteins to mediate leptin's physiological effects.
- Pathways
The Leptin Receptor is integral to the JAK-STAT signaling pathway and the MAPK pathway both of which are important in maintaining energy balance. LEPR activates JAK2 upon ligand binding leading to phosphorylation events that propagate the signal. It is closely associated with other proteins like SOCS3 which acts as a feedback inhibitor of leptin signaling playing a balancing role in the pathways mentioned.
- Associated diseases and disorders
The Leptin Receptor has significant associations with obesity and certain types of diabetes. LEPR mutations or deficiencies can lead to hyperphagia leading to severe obesity due to the disruption of normal leptin signaling. Additionally interactions between leptin and insulin signaling pathways can impact the development of Type 2 diabetes. In these conditions the Leptin Receptor often interacts with the insulin receptor highlighting its role in the complex network of metabolic regulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Leptin Receptor antibody (ab50424)
Lysate in RIPA buffer. Detected by chemiluminescence.
Lane 1: Western blot - Anti-Leptin Receptor antibody (ab50424) at 1.75 µg/mL
Lanes 2 - 3: Western blot - Anti-Leptin Receptor antibody (ab50424) at 1.5 µg/mL
Lane 1: Human Cerebellum tissue lysate at 35 µg
Lane 2: Mouse Foetal Brain at 35 µg
Lane 3: Rat Brain tissue lysate at 35 µg
Flow Cytometry - Anti-Leptin Receptor antibody (ab50424)
Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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