Rabbit Polyclonal Leptin Receptor antibody. Suitable for IP, WB, IHC-Fr and reacts with Mouse, Rat, Human samples. Cited in 35 publications. Immunogen corresponding to Synthetic Peptide within Rat Lepr aa 550-600.
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- International journal of molecular sciences 24:2023Leptin Promotes the Proliferation and Neuronal Differentiation of Neural Stem Cells through the Cooperative Action of MAPK/ERK1/2, JAK2/STAT3 and PI3K/AKT Signaling Pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuolan Tan,Xiaoxuan Hu,Xinyi Wang,Meiqi Sun,Zhenlu Cai,Zixuan Zhang,Yali Fu,Xinlin Chen,Jing An,Haixia LuPubMed 37894835
- BMC complementary medicine and therapies 23:1162023Xiaoyaosan ameliorates depressive-like behavior and susceptibility to glucose intolerance in rat: involvement of LepR-STAT3/PI3K pathway in hypothalamic arcuate nucleus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenqi Qiu,Qian Wu,Kaiwen Zhang,Xiaoli Da,Kairui Tang,Naijun Yuan,Lijuan Deng,Mansi Wu,Ying Zhang,Jiangyan Quan,Qingyu Ma,Xiaojuan Li,Jiaxu ChenPubMed 37046230
- Molecular medicine (Cambridge, Mass.) 28:1592022Hypolipidemic effect of ethanol extract from Chimonanthus nitens Oliv. leaves in hyperlipidemia rats via activation of the leptin/JAK2/STAT3 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianping Pan,Xilin Ouyang,Qi Jin,Wei Wang,Jiali Xie,Baoming Yu,Zhijie Ling,Qizhen Wu,Baoping ZhengPubMed 36539694
- iScience 25:1051712022Inhibition of SRC-mediated integrin signaling in bone marrow niche enhances hematopoietic stem cell function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIrene Mariam Roy,P V Anu,Samantha Zaunz,Srinu Reddi,Aravind M Giri,Rithika Saroj Sankar,Sarah Schouteden,Joerg Huelsken,Catherine M Verfaillie,Satish KhuranaPubMed 36204266
- Frontiers in neuroscience 16:9075082022Leptin and adiponectin regulate the activity of nuclei involved in sleep-wake cycle in male rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOscar Daniel Ramírez-Plascencia,Nadia Saderi,Skarleth Cárdenas-Romero,Fabio García-García,Carolina Peña-Escudero,Omar Flores-Sandoval,Lucia Azuara-Álvarez,Adrián Báez-Ruiz,Roberto Salgado-DelgadoPubMed 35937866
- Cells 11:2022RMR-Related Expression Suppresses Adipogenesis in 3T3-L1 Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuhee Kim,Myoungsook LeePubMed 35456010
- International journal of molecular sciences 23:2022Immature Vascular Smooth Muscle Cells in Healthy Murine Arteries and Atherosclerotic Plaques: Localization and Activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexander Balatskiy,Ilia Ozhimalov,Maria Balatskaya,Alexandra Savina,Julia Filatova,Natalia Kalinina,Vladimir Popov,Vsevolod TkachukPubMed 35163667
- The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology 32:97-1052021Leptin Influence Cholelithiasis Formation by Regulating Bile Acid Metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian Wen,Yu Jiang,Zhengming Lei,Jingyu He,Mingxin Ye,Wenguang FuPubMed 33893772
- Frontiers in cell and developmental biology 8:6070382021Adipokine Leptin Co-operates With Mechanosensitive Ca-Channels and Triggers Actomyosin-Mediated Motility of Breast Epithelial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Acheva,Tytti Kärki,Niccole Schaible,Ramaswamy Krishnan,Sari TojkanderPubMed 33490070
- Cell and tissue research 383:1103-11222020Immunolocalization of leptin and leptin receptor in colorectal mucosa of ulcerative colitis, Crohn's disease and control subjects with no inflammatory bowel disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFlavia Merigo,Alessandro Brandolese,Sonia Facchin,Federico Boschi,Marzia Di Chio,Edoardo Savarino,Renata D'Incà,Giacomo Carlo Sturniolo,Andrea SbarbatiPubMed 33159578
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Rat Lepr aa 550-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q62959
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Reactivity data
Select an application| IP | WB | IHC-Fr | |
|---|---|---|---|
| Human | Expected | Predicted | Expected |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Predicted | Expected |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000 | Notes ab5593 detects an ~100 kDa and ~125 kDa protein representing the short and long forms of OB-R. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Receptor for hormone LEP/leptin (Probable). On ligand binding, mediates LEP central and peripheral effects through the activation of different signaling pathways such as JAK2/STAT3 and MAPK cascade/FOS. In the hypothalamus, LEP acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones (PubMed:8690163). In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic and affects innate and adaptive immunity (By similarity). Control of energy homeostasis and melanocortin production (stimulation of POMC and full repression of AgRP transcription) is mediated by STAT3 signaling, whereas distinct signals regulate NPY and the control of fertility, growth and glucose homeostasis. Involved in the regulation of counter-regulatory response to hypoglycemia by inhibiting neurons of the parabrachial nucleus. Has a specific effect on T lymphocyte responses, differentially regulating the proliferation of naive and memory T-cells. Leptin increases Th1 and suppresses Th2 cytokine production (By similarity). Isoform A. May transport LEP across the blood-brain barrier. Binds LEP and mediates LEP endocytosis (PubMed:10698121). Does not induce phosphorylation of and activate STAT3 (By similarity). Isoform E. Antagonizes Isoform A and isoform B-mediated LEP binding and endocytosis.
Alternative names
CD295, Fa, Obr, Lepr, Leptin receptor, LEP-R, OB receptor, OB-R
Recommended products
Rabbit Polyclonal Leptin Receptor antibody. Suitable for IP, WB, IHC-Fr and reacts with Mouse, Rat, Human samples. Cited in 35 publications. Immunogen corresponding to Synthetic Peptide within Rat Lepr aa 550-600.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Rat Lepr aa 550-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q62959
- Purification technique
- Precipitation Ammonium Sulphate
- Concentration
- Purification notes
No concentration is available.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
As per Uniprot database (P48357) OB receptor has 5 isoforms and is highly glycosylated, this means the observed band size will be different than predicted and multiple bands are expected depending upon the isoforms expressed in sample types.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Leptin Receptor commonly known as LEPR or OB-R is a protein that plays an important role in energy homeostasis and weight regulation. Mechanically it functions as a receptor for leptin the hormone predominantly produced by adipocytes. Leptin binding triggers activation of intracellular signaling pathways. The molecular weight of the Leptin Receptor can vary as it exists in several isoforms due to alternative splicing. It is prominently expressed in the hypothalamus and other tissues involved in energy regulation such as the liver and skeletal muscle.
- Biological function summary
The Leptin Receptor acts as a gateway for leptin to exert its effects on the central nervous system and peripheral tissues. When leptin binds to LEPR it facilitates the formation of receptor complexes that engage downstream signaling molecules like STAT3 and MAPK. This results in regulation of appetite and energy expenditure. LEPR is not part of a complex with other receptors but it interacts with intracellular signaling proteins to mediate leptin's physiological effects.
- Pathways
The Leptin Receptor is integral to the JAK-STAT signaling pathway and the MAPK pathway both of which are important in maintaining energy balance. LEPR activates JAK2 upon ligand binding leading to phosphorylation events that propagate the signal. It is closely associated with other proteins like SOCS3 which acts as a feedback inhibitor of leptin signaling playing a balancing role in the pathways mentioned.
- Associated diseases and disorders
The Leptin Receptor has significant associations with obesity and certain types of diabetes. LEPR mutations or deficiencies can lead to hyperphagia leading to severe obesity due to the disruption of normal leptin signaling. Additionally interactions between leptin and insulin signaling pathways can impact the development of Type 2 diabetes. In these conditions the Leptin Receptor often interacts with the insulin receptor highlighting its role in the complex network of metabolic regulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Leptin Receptor antibody (ab5593)
Western blot detction of both short and long forms of Leptin (OB-R).
All lanes: Western blot - Anti-Leptin Receptor antibody (ab5593) at 1/2000 dilution
All lanes: Mouse lung tissue lysate
Predicted band size: 132 kDa
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