AB5593

Anti-Leptin Receptor antibody

Name: Anti-Leptin Receptor antibody. Rabbit polyclonal (ab5593) | Abcam
Brand: Abcam Limited
SKU: AB5593
Rating: 2 (6 reviews)

(6 Reviews)

(42 Publications)

Rabbit Polyclonal Leptin Receptor antibody. Suitable for IP, WB, IHC-Fr and reacts with Mouse, Rat, Human samples. Cited in 42 publications. Immunogen corresponding to Synthetic Peptide within Rat Lepr aa 550-600.

View Alternative Names

CD295, Fa, Obr, Lepr, Leptin receptor, LEP-R, OB receptor, OB-R

1 Images

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Western blot - Anti-Leptin Receptor antibody (AB5593)

Western blot detction of both short and long forms of Leptin (OB-R).

All lanes:

Western blot - Anti-Leptin Receptor antibody (ab5593) at 1/2000 dilution

All lanes:

Mouse lung tissue lysate

Predicted band size: 132 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IP, IHC-Fr

applications

Immunogen

Synthetic Peptide within Rat Lepr aa 550-600. The exact immunogen used to generate this antibody is proprietary information.

Q62959

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "ab5593 detects an ~100 kDa and ~125 kDa protein representing the short and long forms of OB-R.", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

As per Uniprot database (P48357) OB receptor has 5 isoforms and is highly glycosylated, this means the observed band size will be different than predicted and multiple bands are expected depending upon the isoforms expressed in sample types.

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Properties and storage information

Form

Liquid

Purification technique

Precipitation Ammonium Sulphate

Purification notes

No concentration is available.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Leptin Receptor commonly known as LEPR or OB-R is a protein that plays an important role in energy homeostasis and weight regulation. Mechanically it functions as a receptor for leptin the hormone predominantly produced by adipocytes. Leptin binding triggers activation of intracellular signaling pathways. The molecular weight of the Leptin Receptor can vary as it exists in several isoforms due to alternative splicing. It is prominently expressed in the hypothalamus and other tissues involved in energy regulation such as the liver and skeletal muscle.

Biological function summary

The Leptin Receptor acts as a gateway for leptin to exert its effects on the central nervous system and peripheral tissues. When leptin binds to LEPR it facilitates the formation of receptor complexes that engage downstream signaling molecules like STAT3 and MAPK. This results in regulation of appetite and energy expenditure. LEPR is not part of a complex with other receptors but it interacts with intracellular signaling proteins to mediate leptin's physiological effects.

Pathways

The Leptin Receptor is integral to the JAK-STAT signaling pathway and the MAPK pathway both of which are important in maintaining energy balance. LEPR activates JAK2 upon ligand binding leading to phosphorylation events that propagate the signal. It is closely associated with other proteins like SOCS3 which acts as a feedback inhibitor of leptin signaling playing a balancing role in the pathways mentioned.

The Leptin Receptor has significant associations with obesity and certain types of diabetes. LEPR mutations or deficiencies can lead to hyperphagia leading to severe obesity due to the disruption of normal leptin signaling. Additionally interactions between leptin and insulin signaling pathways can impact the development of Type 2 diabetes. In these conditions the Leptin Receptor often interacts with the insulin receptor highlighting its role in the complex network of metabolic regulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for hormone LEP/leptin (Probable). On ligand binding, mediates LEP central and peripheral effects through the activation of different signaling pathways such as JAK2/STAT3 and MAPK cascade/FOS. In the hypothalamus, LEP acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones (PubMed : 8690163). In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic and affects innate and adaptive immunity (By similarity). Control of energy homeostasis and melanocortin production (stimulation of POMC and full repression of AgRP transcription) is mediated by STAT3 signaling, whereas distinct signals regulate NPY and the control of fertility, growth and glucose homeostasis. Involved in the regulation of counter-regulatory response to hypoglycemia by inhibiting neurons of the parabrachial nucleus. Has a specific effect on T lymphocyte responses, differentially regulating the proliferation of naive and memory T-cells. Leptin increases Th1 and suppresses Th2 cytokine production (By similarity).. Isoform A. May transport LEP across the blood-brain barrier. Binds LEP and mediates LEP endocytosis (PubMed : 10698121). Does not induce phosphorylation of and activate STAT3 (By similarity).. Isoform E. Antagonizes Isoform A and isoform B-mediated LEP binding and endocytosis.

See full target information Lepr

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Publications (42)

Recent publications for all applications. Explore the full list and refine your search

Nature and science of sleep 16:2029-2043 PubMed39712883

2024

Impact of Chronic Intermittent Hypoxia on Cognitive Function and Hippocampal Neurons in Mice: A Study of Inflammatory and Oxidative Stress Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Zhang,Dandi Ma,Yunxiao Wu,Zhifei Xu

Glia 73:25-37 PubMed39310943

2024

Leptin reduces LPS-induced A1 reactive astrocyte activation and inflammation via inhibiting p38-MAPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Meiqi Sun,Yiqun Song,Xiaoxuan Hu,Zixuan Zhang,Ruolan Tan,Zhenlu Cai,Xinyi Wang,Yali Fu,Hongli You,Simeng Cui,Wanting Zhao,Jing An,Xinlin Chen,Haixia Lu

Cancer research and treatment 57:457-477 PubMed39164083

2024

Synergistic Activation of LEPR and ADRB2 Induced by Leptin Enhances Reactive Oxygen Specie Generation in Triple-Negative Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Liu,Jing Yu,Yongjun Du,Yu Xie,Xiaofei Song,Chang Liu,Yan Yan,Yue Wang,Junfang Qin

Pharmaceuticals (Basel, Switzerland) 17: PubMed39065712

2024

Fish Oil Supplementation Mitigates High-Fat Diet-Induced Obesity: Exploring Epigenetic Modulation and Genes Associated with Adipose Tissue Dysfunction in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jussara de Jesus Simão,Andressa França de Sousa Bispo,Victor Tadeu Gonçalves Plata,Lucia Maria Armelin-Correa,Maria Isabel Cardoso Alonso-Vale

Andrology 13:371-381 PubMed38778669

2024

Cellular mechanism underlying leptin-induced anion secretion of rat epididymal epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dong-Dong Gao,Guo-Qing Liu,Yi-Lin Chen,Nan Ding,Jia-Hui Zhong,Guang-Nan Liang,Wei-Ji Deng,Pei-Lun Li,Jia-Rui Su,Ming Wang,Jun-Hao Huang,Min Hu

International journal of molecular sciences 24: PubMed37894835

2023

Leptin Promotes the Proliferation and Neuronal Differentiation of Neural Stem Cells through the Cooperative Action of MAPK/ERK1/2, JAK2/STAT3 and PI3K/AKT Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Ruolan Tan,Xiaoxuan Hu,Xinyi Wang,Meiqi Sun,Zhenlu Cai,Zixuan Zhang,Yali Fu,Xinlin Chen,Jing An,Haixia Lu

BMC complementary medicine and therapies 23:116 PubMed37046230

2023

Xiaoyaosan ameliorates depressive-like behavior and susceptibility to glucose intolerance in rat: involvement of LepR-STAT3/PI3K pathway in hypothalamic arcuate nucleus.

Applications

Unspecified application

Species

Unspecified reactive species

Wenqi Qiu,Qian Wu,Kaiwen Zhang,Xiaoli Da,Kairui Tang,Naijun Yuan,Lijuan Deng,Mansi Wu,Ying Zhang,Jiangyan Quan,Qingyu Ma,Xiaojuan Li,Jiaxu Chen

Cell death & disease 14:82 PubMed36737598

2023

Metformin induces pyroptosis in leptin receptor-defective hepatocytes via overactivation of the AMPK axis.

Applications

Unspecified application

Species

Unspecified reactive species

Bingli Liu,Jingyuan Xu,Linyao Lu,Lili Gao,Shengjuan Zhu,Yi Sui,Ting Cao,Tao Yang

Molecular medicine (Cambridge, Mass.) 28:159 PubMed36539694

2022

Hypolipidemic effect of ethanol extract from Chimonanthus nitens Oliv. leaves in hyperlipidemia rats via activation of the leptin/JAK2/STAT3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianping Pan,Xilin Ouyang,Qi Jin,Wei Wang,Jiali Xie,Baoming Yu,Zhijie Ling,Qizhen Wu,Baoping Zheng

iScience 25:105171 PubMed36204266

2022

Inhibition of SRC-mediated integrin signaling in bone marrow niche enhances hematopoietic stem cell function.

Applications

Unspecified application

Species

Unspecified reactive species

Irene Mariam Roy,P V Anu,Samantha Zaunz,Srinu Reddi,Aravind M Giri,Rithika Saroj Sankar,Sarah Schouteden,Joerg Huelsken,Catherine M Verfaillie,Satish Khurana

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Leptin Receptor antibody

Western blot - Anti-Leptin Receptor antibody (AB5593)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (42)

Product promise