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AB281234

Anti-Leptin Receptor antibody [EPR23802-47] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal Leptin Receptor antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

CD295, DB, OBR, LEPR, Leptin receptor, LEP-R, HuB219, OB receptor, OB-R

1 Images
Sandwich ELISA - Anti-Leptin Receptor antibody [EPR23802-47] - BSA and Azide free (Capture) (AB281234)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Leptin Receptor antibody [EPR23802-47] - BSA and Azide free (Capture) (AB281234)

Example of Human Leptin Receptor standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23802-47

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab281234 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify human LEPR/Leptin R. The recommended pair for sandwich ELISA is:
Capture: ab281234, human LEPR/Leptin R Capture Antibody (unconjugated)
Detector: ab281084, human LEPR/Leptin R Detector Antibody (unconjugated)
The reference range value is 156 - 5000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Leptin Receptor commonly known as LEPR or OB-R is a protein that plays an important role in energy homeostasis and weight regulation. Mechanically it functions as a receptor for leptin the hormone predominantly produced by adipocytes. Leptin binding triggers activation of intracellular signaling pathways. The molecular weight of the Leptin Receptor can vary as it exists in several isoforms due to alternative splicing. It is prominently expressed in the hypothalamus and other tissues involved in energy regulation such as the liver and skeletal muscle.
Biological function summary

The Leptin Receptor acts as a gateway for leptin to exert its effects on the central nervous system and peripheral tissues. When leptin binds to LEPR it facilitates the formation of receptor complexes that engage downstream signaling molecules like STAT3 and MAPK. This results in regulation of appetite and energy expenditure. LEPR is not part of a complex with other receptors but it interacts with intracellular signaling proteins to mediate leptin's physiological effects.

Pathways

The Leptin Receptor is integral to the JAK-STAT signaling pathway and the MAPK pathway both of which are important in maintaining energy balance. LEPR activates JAK2 upon ligand binding leading to phosphorylation events that propagate the signal. It is closely associated with other proteins like SOCS3 which acts as a feedback inhibitor of leptin signaling playing a balancing role in the pathways mentioned.

The Leptin Receptor has significant associations with obesity and certain types of diabetes. LEPR mutations or deficiencies can lead to hyperphagia leading to severe obesity due to the disruption of normal leptin signaling. Additionally interactions between leptin and insulin signaling pathways can impact the development of Type 2 diabetes. In these conditions the Leptin Receptor often interacts with the insulin receptor highlighting its role in the complex network of metabolic regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for hormone LEP/leptin (Probable) (PubMed : 22405007). On ligand binding, mediates LEP central and peripheral effects through the activation of different signaling pathways such as JAK2/STAT3 and MAPK cascade/FOS. In the hypothalamus, LEP acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones (By similarity) (PubMed : 9537324). In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic and affects innate and adaptive immunity (PubMed : 12504075, PubMed : 25060689, PubMed : 8805376). Control of energy homeostasis and melanocortin production (stimulation of POMC and full repression of AgRP transcription) is mediated by STAT3 signaling, whereas distinct signals regulate NPY and the control of fertility, growth and glucose homeostasis. Involved in the regulation of counter-regulatory response to hypoglycemia by inhibiting neurons of the parabrachial nucleus. Has a specific effect on T lymphocyte responses, differentially regulating the proliferation of naive and memory T -ells. Leptin increases Th1 and suppresses Th2 cytokine production (By similarity).. Isoform A. May transport LEP across the blood-brain barrier. Binds LEP and mediates LEP endocytosis. Does not induce phosphorylation of and activate STAT3.. Isoform E. Antagonizes Isoform A and isoform B-mediated LEP binding and endocytosis.
See full target information LEPR
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Product promise

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