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AB69486

Anti-Lethal factor antibody [LF2]

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(1 Publication)

Mouse Monoclonal Lethal factor antibody. Suitable for IP, ELISA, WB and reacts with Bacillus anthracis samples. Cited in 1 publication. Immunogen corresponding to Full Length Protein corresponding to Bacillus anthracis lef.

View Alternative Names

pXO1-107, BXA0172, GBAA_pXO1_0172, lef, Lethal factor, LF, Anthrax lethal toxin endopeptidase component

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

LF2

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Bacillus anthracis

Applications

IP, ELISA, WB

applications

Immunogen

Full Length Protein corresponding to Bacillus anthracis lef. The exact immunogen used to generate this antibody is proprietary information.

P15917

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Bacillus anthracis": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Lethal Factor also known as Anthrax Lethal Factor is an important component of the anthrax toxin produced by Bacillus anthracis. It has a molecular mass of approximately 90 kDa. This protein operates as a zinc-dependent metalloprotease and cleaves specific mitogen-activated protein kinase kinases (MAPKKs). The Lethal Factor along with Protective Antigen and Edema Factor form the tripartite Anthrax Toxin complex. While the Protective Antigen enables entry into host cells the Lethal Factor executes the proteolytic function. It is predominately expressed in conditions where Bacillus anthracis infects mammalian hosts enabling its toxic potential.
Biological function summary

Lethal Factor interferes with intracellular cell signaling pathways important for immune response. By specifically targeting MAPKKs it disrupts the regulation of critical cellular processes including cell growth differentiation and survival. The complex formed by Lethal Factor and Protective Antigen allows the toxin to enter the host cell and exert its deleterious effects. The enzymatic activity of Lethal Factor depends on its ability to enter cells where it performs in conjunction with other proteins like the Edema Factor.

Pathways

Several cellular processes become affected by the Lethal Factor's activity. This protein disrupts the MAPK signaling pathway which is essential for cellular responses such as proliferation differentiation and stress response. Also it combines with Edema Factor to contribute to pathophysiology by amplifying cell dysregulation. By interacting with MAPKK enzymes the activity of downstream kinases such as ERK1/2 and JNK get inhibited which in turn affects cytokine production and cell survival mechanisms.

Lethal Factor is closely associated with Anthrax disease a zoonotic disease that primarily affects herbivorous animals but can infect humans. Lethal Factor contributes to the severity of the disease by impeding immune system function and disrupting cellular homeostasis. The protein interacts with Protective Antigen to form lethal toxin an important player in the pathogenesis of the anthrax disease. Through disruption of signaling pathways and immune modulation these two protein factors foster the development of systemic shock and mortality.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Lethal factor (LF), which constitutes one of the three proteins composing the anthrax toxin, is able to trigger rapid cell death in macrophages (PubMed : 10475971, PubMed : 11104681, PubMed : 3711080, PubMed : 8380282, PubMed : 9563949, PubMed : 9703991). Acts as a protease that cleaves the N-terminal of most dual specificity mitogen-activated protein kinase kinases (MAPKKs or MAP2Ks) (except for MAP2K5) : cleavage invariably occurs within the N-terminal proline-rich region preceding the kinase domain, thus disrupting a sequence involved in directing specific protein-protein interactions necessary for the assembly of signaling complexes (PubMed : 10475971, PubMed : 11104681, PubMed : 14718925, PubMed : 9563949, PubMed : 9703991). Also cleaves mouse Nlrp1b : host Nlrp1b cleavage promotes ubiquitination and degradation of the N-terminal part of Nlrp1b by the proteasome, thereby releasing the cleaved C-terminal part of Nlrp1b, which polymerizes and forms the Nlrp1b inflammasome followed by host cell pyroptosis (PubMed : 10338520, PubMed : 19651869, PubMed : 30872531, PubMed : 31268597). Able to cleave mouse Nlrp1b alleles 1 and 5, while it is not able to cleave Nlrp1b alleles 2, 3 and 4 (PubMed : 16429160, PubMed : 19651869). In contrast, does not cleave NLRP1 human ortholog (PubMed : 19651869). LF is not toxic by itself and only acts as a lethal factor when associated with protective antigen (PA) to form the lethal toxin (LeTx) : PA is required for LF translocation into the host cytosol (PubMed : 10475971, PubMed : 11104681, PubMed : 9563949, PubMed : 9703991).
See full target information lef

Additional targets

Lethal factor
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of microbiology and biotechnology 35:e2503036 PubMed40443240

2025

A Nasal Vaccine Displaying Anthrax Antigen on the Surface of Induces Protective Mucosal Immunity against Anthrax Toxin.

Applications

Unspecified application

Species

Unspecified reactive species

In-Hwan Jang,In Ryeong Jung,Hyojung Suh,Hyo-Joo Ahn,Donghyun Kim,Youn Soo Choi,June-Yong Lee,Kyung-Ah Lee
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