Rabbit Polyclonal LETM1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Mitochondrial proton/calcium exchanger protein aa 600-700.
View Alternative Names
Mitochondrial proton/calcium exchanger protein, Electroneutral mitochondrial K(+)/H(+)exchanger, Leucine zipper-EF-hand-containing transmembrane protein 1, KHE, LETM1
- IP
Supplier Data
Immunoprecipitation - Anti-LETM1 antibody (AB241155)
LETM1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241155 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241155 at 0.4 μg/ml.
Lane 1 : ab241155 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.
Detection : Chemiluminescence with exposure time of 10 seconds.
All lanes:
Immunoprecipitation - Anti-LETM1 antibody (ab241155)
Predicted band size: 83 kDa
false
- WB
Supplier Data
Western blot - Anti-LETM1 antibody (AB241155)
Lysates prepared using NETN buffer.
All lanes:
Western blot - Anti-LETM1 antibody (ab241155) at 0.1 µg/mL
Lane 1:
HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 2:
HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 3:
Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg
Predicted band size: 83 kDa
true
Exposure time: 30s
Complementary Products
Reactivity data
Properties and storage information
Form
Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
LETM1 contributes to maintaining mitochondrial function and integrity. It comprises part of a complex that includes other mitochondrial membrane proteins. This complex ensures sufficient ion gradients and energy production within the cell. Its role in calcium ion homeostasis aids in regulating mitochondrial membrane potential and ATP synthesis. Efficient operation of this mechanism is essential for cell survival and function.
Pathways
LETM1 integrates into metabolic pathways where it supports mitochondrial bioenergetics and oxidative phosphorylation. It interacts with the OXPHOS system during energy production. LETM1's activity also affects the cellular apoptosis pathway influenced by its regulation of calcium levels. Related proteins like MICU1 and MCU participate in these pathways contributing to the regulation of calcium uptake into mitochondria and further connecting LETM1’s role to broader cellular processes.
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Target data
Publications (1)
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Oxidative medicine and cellular longevity 2022:8825784 PubMed35281474
2022
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
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