Rabbit Polyclonal LETM1 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human Mitochondrial proton/calcium exchanger protein aa 600-700.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human Mitochondrial proton/calcium exchanger protein aa 600-700. The exact immunogen used to generate this antibody is proprietary information. Database link O95202
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Target data
Function
Plays an important role in maintenance of mitochondrial morphology and in mediating either calcium or potassium/proton antiport (PubMed:18628306, PubMed:19797662, PubMed:24344246, PubMed:24898248, PubMed:29123128, PubMed:32139798, PubMed:36055214, PubMed:36321428). Mediates proton-dependent calcium efflux from mitochondrion (PubMed:19797662, PubMed:24344246, PubMed:29123128). Functions also as an electroneutral mitochondrial proton/potassium exchanger (PubMed:24898248, PubMed:36055214, PubMed:36321428). Crucial for the maintenance of mitochondrial tubular networks and for the assembly of the supercomplexes of the respiratory chain (PubMed:18628306, PubMed:36055214). Required for the maintenance of the tubular shape and cristae organization (PubMed:18628306, PubMed:32139798).
Alternative names
Mitochondrial proton/calcium exchanger protein, Electroneutral mitochondrial K(+)/H(+)exchanger, Leucine zipper-EF-hand-containing transmembrane protein 1, KHE, LETM1
Recommended products
Rabbit Polyclonal LETM1 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human Mitochondrial proton/calcium exchanger protein aa 600-700.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human Mitochondrial proton/calcium exchanger protein aa 600-700. The exact immunogen used to generate this antibody is proprietary information. Database link O95202
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Antibody was affinity purified using an epitope specific to LETM1 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LETM1 short for Leucine Zipper and EF-Hand Containing Transmembrane Protein 1 plays an important role in cellular ion regulation. This protein with a mass of approximately 81 kDa is mainly located in the inner mitochondrial membrane. LETM1 acts as a mitochondrial calcium and potassium exchanger. It facilitates the transport of calcium out of the mitochondria balancing mitochondrial ion homeostasis. Expression of LETM1 occurs widely across different tissue types indicating its importance in basic cellular function.
- Biological function summary
LETM1 contributes to maintaining mitochondrial function and integrity. It comprises part of a complex that includes other mitochondrial membrane proteins. This complex ensures sufficient ion gradients and energy production within the cell. Its role in calcium ion homeostasis aids in regulating mitochondrial membrane potential and ATP synthesis. Efficient operation of this mechanism is essential for cell survival and function.
- Pathways
LETM1 integrates into metabolic pathways where it supports mitochondrial bioenergetics and oxidative phosphorylation. It interacts with the OXPHOS system during energy production. LETM1's activity also affects the cellular apoptosis pathway influenced by its regulation of calcium levels. Related proteins like MICU1 and MCU participate in these pathways contributing to the regulation of calcium uptake into mitochondria and further connecting LETM1’s role to broader cellular processes.
- Associated diseases and disorders
LETM1 is linked to Wolf-Hirschhorn syndrome a genetic disorder characterized by developmental delays and seizures. Mutations or deletions affecting LETM1 contribute significantly to the syndrome's phenotype. Additionally LETM1 dysregulation associates with certain types of cancer where aberrant calcium signaling may promote tumor progression. The connection between LETM1 and proteins like Cyclophilin D involved in mitochondrial integrity highlights its influence in disease mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-LETM1 antibody (ab241155)
Lysates prepared using NETN buffer.
All lanes: Western blot - Anti-LETM1 antibody (ab241155) at 0.1 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 3: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 83 kDa
Exposure time: 30s
Immunoprecipitation - Anti-LETM1 antibody (ab241155)
LETM1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241155 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241155 at 0.4 μg/ml.
Lane 1: ab241155 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence with exposure time of 10 seconds.
All lanes: Immunoprecipitation - Anti-LETM1 antibody (ab241155)
Predicted band size: 83 kDa
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