AB248355

Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Leukotriene B4 Receptor/BLT antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

BLT, BLT1, BLTR, CMKRL1, GPR16, P2RY7, LTB4R, Leukotriene B4 receptor 1, LTB4-R 1, LTB4-R1, Chemoattractant receptor-like 1, G-protein coupled receptor 16, P2Y purinoceptor 7, P2Y7

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] - BSA and Azide free (AB248355)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] - BSA and Azide free (AB248355)

This data was developed using ab131041, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Leukotriene B4 Receptor/BLT with ab131041 antibody at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] - BSA and Azide free (AB248355)

This data was developed using ab131041, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling Leukotriene B4 Receptor/BLT with ab131041 antibody at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] - BSA and Azide free (AB248355)

This data was developed using ab131041, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] (<a href='/en-us/products/primary-antibodies/leukotriene-b4-receptor-blt-antibody-epr7113-ab131041'>ab131041</a>) at 1/10000 dilution

Lane 1:

Human thymus lysate at 10 µg

Lane 2:

Human fetal lung tissue lysate at 10 µg

Lane 3:

A549 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 38 kDa

Observed band size: 38 kDa

false

OI-RD Scanning - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] - BSA and Azide free (AB248355)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] - BSA and Azide free (AB248355)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Unconjugated

Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7113

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab248355 is the carrier-free version of ab131041.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Leukotriene B4 Receptor often referred to as BLT or BLT1 plays a significant mechanical role in the immune response by binding to leukotriene B4 (LTB4) a potent chemoattractant. This receptor has a molecular mass of about 45 kDa. It expresses primarily on leukocytes including neutrophils macrophages and certain T cells. Furthermore BLT1 can also be observed in other tissues such as the spleen and liver where immune modulation has essential roles.

Biological function summary

The function of BLT1 involves mediating cell migration particularly during inflammatory responses. When LTB4 binds to BLT1 it activates intracellular signaling pathways that lead to chemotaxis and activation of immune cells. This receptor does not function in isolation; it forms part of receptor complexes on the cell surface to facilitate effective signal transduction. Although its name suggests specificity BLT1 can interact with other related lipid mediators amplifying its role in inflammation.

Pathways

Leukotriene B4 receptor BLT1 is integral to the arachidonic acid pathway and the GPCR signaling pathway. In the arachidonic acid pathway BLT1 contributes to the production of inflammatory mediators that recruit immune cells to sites of injury or infection. This receptor shares pathways with other GPCRs facilitating coordinated responses to external stimuli. It is closely related to protein kinase C (PKC) in the pathways which plays a role in signal transduction and cellular communication.

Leukotriene B4 receptor BLT1 has significant implications in chronic inflammatory diseases such as asthma and rheumatoid arthritis. Overactivation of BLT1 can result in excessive recruitment of immune cells exacerbating inflammation and tissue damage in these conditions. The receptor is also connected to COX-2 which is involved in the inflammation process linking BLT1 to the broader picture of inflammatory pathways in disease states. Understanding BLT1's role in these diseases helps identify potential therapeutic targets for managing inflammation-related pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for extracellular ATP > UTP and ADP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. May be the cardiac P2Y receptor involved in the regulation of cardiac muscle contraction through modulation of L-type calcium currents. Is a receptor for leukotriene B4, a potent chemoattractant involved in inflammation and immune response.

See full target information LTB4R

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] - BSA and Azide free (AB248355)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] - BSA and Azide free (AB248355)

Western blot - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] - BSA and Azide free (AB248355)

OI-RD Scanning - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] - BSA and Azide free (AB248355)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise