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AB67571

Anti-Leupaxin antibody

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(1 Publication)

Mouse Polyclonal Leupaxin antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human LPXN.

View Alternative Names

LDLP, LPXN, Leupaxin

2 Images
Western blot - Anti-Leupaxin antibody (AB67571)
  • WB

Unknown

Western blot - Anti-Leupaxin antibody (AB67571)

All lanes:

Western blot - Anti-Leupaxin antibody (ab67571) at 1/500 dilution

All lanes:

human spleen tissue lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 43 kDa

Observed band size: 43 kDa

false

Western blot - Anti-Leupaxin antibody (AB67571)
  • WB

Unknown

Western blot - Anti-Leupaxin antibody (AB67571)

All lanes:

Western blot - Anti-Leupaxin antibody (ab67571) at 1/500 dilution

Lane 1:

Leupaxin transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 43 kDa

Observed band size: 43 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human LPXN.

O60711

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Leupaxin is a cytoskeletal-associated protein involved in cellular adhesion and migration. Also known as LPXN it belongs to the paxillin superfamily and has a molecular weight of approximately 42 kDa. The expression of leupaxin is mainly found in hematopoietic cells including leukocytes. It contains LIM domains and an LD motif which play significant roles in protein-protein interactions.
Biological function summary

The protein plays an important role in regulating actin cytoskeleton dynamics and cell spreading. It interacts with various signaling complexes and integrates into focal adhesions. Leupaxin often participates with kinases such as FAK and Src to modulate cellular responses to external stimuli. Its colocalization with focal adhesion proteins suggests involvement in mechanotransduction processes.

Pathways

Proteins like leupaxin participate in pathways regulating cell migration and adhesion. One notable pathway is the integrin signaling pathway influencing diverse cellular processes. It associates with proteins like talin and vinculin essential for the linkage between integrins and the cytoskeleton. These interactions help in modifying cytoskeletal reorganization and cellular signaling cascades.

Leupaxin has relevance in cancer particularly in metastatic processes and autoimmune disorders. Its role in promoting or inhibiting cell migration ties into metastatic cancer's progression where it possibly interacts with proteins like paxillin. In autoimmune disorders aberrant function or expression of leupaxin could affect immune cell mobility and contribute to disease pathology by modifying immune cell adhesion properties.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional coactivator for androgen receptor (AR) and serum response factor (SRF). Contributes to the regulation of cell adhesion, spreading and cell migration and acts as a negative regulator in integrin-mediated cell adhesion events. Suppresses the integrin-induced tyrosine phosphorylation of paxillin (PXN). May play a critical role as an adapter protein in the formation of the adhesion zone in osteoclasts. Negatively regulates B-cell antigen receptor (BCR) signaling.
See full target information LPXN
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 17:135-140 PubMed30655748

2019

Downregulation of LPXN expression by siRNA decreases the malignant proliferation and transmembrane invasion of SHI-1 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Guo-Hua Zhu,Hai-Ping Dai,Qun Shen,Qi Zhang
View all publications
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Product promise

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