JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB127754

Anti-LeuRS antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal LeuRS antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human LARS1 aa 900 to C-terminus.

View Alternative Names

KIAA1352, LARS, LARS1, Leucyl-tRNA synthetase, LeuRS, cLRS

1 Images
Western blot - Anti-LeuRS antibody (AB127754)
  • WB

Unknown

Western blot - Anti-LeuRS antibody (AB127754)

5% SDS PAGE

All lanes:

Western blot - Anti-LeuRS antibody (ab127754) at 1/1000 dilution

Lane 1:

NT2D1 whole cell lysate at 30 µg

Lane 2:

PC 3 whole cell lysate at 30 µg

Lane 3:

U87 MG whole cell lysate at 30 µg

Lane 4:

SK N SH whole cell lysate at 30 µg

Predicted band size: 134 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human LARS1 aa 900 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9P2J5

style
left-column

Complementary Products

Primary Antibodies

AB36569

Anti-Reptin/TIP49B/RUVB2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Reptin/TIP49B/RUVB2 antibody (AB36569)

  • 4
  • 13
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB316107

Anti-VDAC2 antibody [EPR27028-135] - Mitochondrial Loading Control

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC2 antibody [EPR27028-135] - Mitochondrial Loading Control (AB316107)

  • 0
  • 0
Primary Antibodies

AB68155

Anti-SOD2/MnSOD antibody [EPR2560Y]

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (AB68155)

  • 5
  • 2
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

Keep as concentrated solution.
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Leucyl-tRNA synthetase (LeuRS) also known as LARS is an enzyme that plays an essential role in protein synthesis. This enzyme catalyzes the attachment of leucine to its corresponding tRNA a critical step in translating genetic information into proteins. LeuRS has an approximate molecular weight of 120 kDa for its cytoplasmic form. LeuRS expresses in various cellular compartments primarily in the cytoplasm and mitochondria indicating its importance in both locations for the assembly of proteins.
Biological function summary

Leucyl-tRNA synthetase is a vital component of the cellular translation machinery ensuring the accuracy of protein biosynthesis. It is responsible for the charging of tRNA with the amino acid leucine which is an important step in maintaining the fidelity of genetic code translation. LeuRS operates as a monomer and does not form larger complexes. Its role highlights the importance of aminoacyl-tRNA synthetases in connecting nucleic acid information with amino acid sequences a fundamental process in living organisms.

Pathways

Leucyl-tRNA synthetase is significantly involved in the aminoacyl-tRNA biosynthesis pathway and protein translation. This pathway is critical for synthesizing proteins involving several enzymes like methionyl-tRNA synthetase and phenylalanyl-tRNA synthetase which work alongside LeuRS. By facilitating the correct attachment of leucine LeuRS contributes to the efficiency and precision of the translation process within the cellular environment.

Leucyl-tRNA synthetase is linked to certain mitochondrial dysfunctions and cancer. Variations or mutations in LeuRS have been associated with conditions such as infantile hypertrophic cardiomyopathy due to its mitochondrial role. In cancer altered expression or mutations in LeuRS might influence pathways related to protein synthesis and cell proliferation potentially in connection with proteins like p53 that regulate cell growth and apoptosis. Understanding these interactions can provide insights for therapeutic strategies targeting translation-related abnormalities.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Aminoacyl-tRNA synthetase that catalyzes the specific attachment of leucine to its cognate tRNA (tRNA(Leu)) (PubMed : 25051973, PubMed : 32232361). It performs tRNA aminoacylation in a two-step reaction : Leu is initially activated by ATP to form a leucyl-adenylate (Leu-AMP) intermediate; then the leucyl moiety is transferred to the acceptor 3' end of the tRNA to yield leucyl-tRNA (PubMed : 25051973). To improve the fidelity of catalytic reactions, it is also able to hydrolyze misactivated aminoacyl-adenylate intermediates (pre-transfer editing) and mischarged aminoacyl-tRNAs (post-transfer editing) (PubMed : 25051973).
See full target information LARS1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Proteome science 19:3 PubMed33588889

2021

Quantitative proteomic profiling of Cervicovaginal fluid from pregnant women with term and preterm birth.

Applications

Unspecified application

Species

Unspecified reactive species

Young Eun Kim,Kwonseong Kim,Han Bin Oh,Sung Ki Lee,Dukjin Kang
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com